LncRNA IMFlnc1 Promotes Porcine Intramuscular Adipocyte Adipogenesis by Sponging Mir-199a-5p to Up-regulate CAV-1

Abstract

Background: Local Chinese local pig breeds have thinner muscle fiber and higher intramuscular-fat (IMF) content. But its regulation mechanism has not been discussed in-depth. Studies indicated that long non coding RNAs (lncRNAs) play important role in muscle and fat development.Results: The lncRNAs expressional differences in the longissimus dorsi (LD) muscle were identified between Huainan pigs (local Chinese pigs, fat-type, HN) and Large White pigs (lean-type, LW) at 38, 58, and 78 days post conception (dpc). In total, 2131 novel lncRNAs were identified in 18 samples, and 291, 305, and 683 differentially expressed lncRNAs (DELs) were found between these two breeds at three stages, respectively. GO and KEGG analysis of the DEL co expressed mRNAs showed that muscle development and energy metabolism were more active at 58 dpc in HN, but at 78 dpc in LW pigs. Muscle cell differentiation and myofibril assembly might associated with earlier myogenesis and primary-muscle-fiber assembly in HN, and cell proliferation, insulin, and the MAPK pathway might contribute to longer proliferation in LW pigs. The PI3K/Akt and cAMP pathways were associated with higher IMF deposition in HN. IMFlnc1 was selected for functional verification, and results indicated that it regulated the expressional level of caveolin-1 (CAV-1) by acting as competing endogenous RNA (ceRNA) to sponge miR-199a-5p.Conclusions: Our data contributed to understanding lncRNAs in porcine-muscle development and IMF deposition, and provided valuable information for improving pig-meat quality.

Competing endogenous RNA network construction based on long non‐coding RNAs, microRNAs, and mRNAs related to fat deposition in Songliao black swine

“…These results are consistent with those of previous studies. Wang, Chen, et al (2020), Wang, Zhang, et al (2020), Wang, Zhong, et al (2020) analyzed the liver transcriptomes of Yorkshire pigs and Anqing six‐end‐white pigs and identified 362 differentially expressed genes. These genes were significantly enriched in fat‐related GO terms and KEGG pathways, such as the lipid metabolic process and PPAR signaling pathway.…”

“…Previous studies have explored ceRNA networks that regulate fat deposition traits in different species. Wang, Chen, et al (2020), Wang, Zhang, et al (2020), Wang, Zhong, et al (2020) performed high‐throughput sequencing on adipose tissues of the 0.5‐ and 2.5‐year‐old yaks, and constructed a ceRNA network related to intramuscular fat deposition, contained 10 mRNAs, 5 miRNAs, 5 circRNAs, 3 lncRNAs, and 29 relationships. Chen et al (2019) constructed a ceRNA network of 96 lncRNAs, 71 miRNAs, and 84 mRNAs involved in adipogenic differentiation of chicken preadipocytes, and eight of which have been experimentally verified.…”

“…Chen et al (2019) constructed a ceRNA network of 96 lncRNAs, 71 miRNAs, and 84 mRNAs involved in adipogenic differentiation of chicken preadipocytes, and eight of which have been experimentally verified. Wang, Chen, et al (2020), Wang, Zhang, et al (2020), Wang, Zhong, et al (2020) used pig primary adipocytes as materials to verify the molecular mechanism of lncRNA IMFlnc1: regulate the expression level of gene caveolin‐1 (CAV‐1) by acting as ceRNA to adsorb miR‐199a‐5p, and further promote the differentiation of pig intramuscular adipocytes.…”