lncRNA IGF2 AS Regulates Bovine Myogenesis through Different Pathways

Abstract

The role of long non-coding RNA (lncRNA) in the regulation of bovine skeletal muscle development remains poorly understood. The present study investigated the function and regulatory mechanism of a novel lncRNA, insulin-like growth factor 2 antisense transcript (IGF2 AS), in bovine myoblast proliferation and differentiation. Gain or loss of IGF2 AS was performed using an expression plasmid or small interfering RNA (siRNA), respectively. Bovine myoblasts were used to investigate the biological function and mechanisms of IGF2 AS in vitro. Results were conjointly analyzed by celluar and molecular biology experiments as well as bioinformatics. Functionally, IGF2 AS could promote proliferation and differentiation of bovine myoblasts. The preliminary mechanism suggests, on the one hand, that IGF2 AS could complement the IGF2 gene intron region and affect the stability and expression of IGF2 mRNA. On the other hand, RNA pull-down and immunoprecipitation assays demonstrated that IGF2 AS could directly bind to the interleukin enhancer binding factor 3 (ILF3) protein and maybe partly though it to regulate myogenesis. In conclusion, the novel identified lncRNA IGF2 AS promoted proliferation and differentiation of bovine myoblasts through various pathways.

lncSAMM50 Enhances Adipogenic Differentiation of Buffalo Adipocytes With No Effect on Its Host Gene

“…Generally, the gene is expressed in a time- and stage-specific manner and is regulated by multiple factors. With the development of RNA sequencing, the lncRNA expression profile has been characterized in multiple tissues in livestock animals ( Huang et al, 2019 ; Li et al, 2020 ; Song et al, 2020 ). In the present study, the comparison of the lncRNA expression profiles of adipose and muscle tissues identified 241 DE lncRNAs ( Supplementary Table 3 ).…”

“…The existing data suggest that lncRNA can play a role by regulating the expression of a host gene ( Guo et al, 2019 ; Song et al, 2020 ), meaning that the function of a lncRNA is associated with its host gene. SAMM50 is the core component of the sorting and assembly machinery and plays a critical role in regulating mitochondrial dynamics and mitophagy ( Liu et al, 2016 ; Jian et al, 2018 ), indicating a significant role of SAMM50 in energy metabolism.…”

“…Existing evidence suggests that lncRNAs can repress or activate the hose gene in the cis method ( Fatica and Bozzoni, 2014 ; Wang et al, 2016 ; Song et al, 2020 ). Sirt1 antisense (AS) lncRNA is transcribed from the AS strand of the Sirt1 gene.…”

“…Sirt1 AS lncRNA promotes myoblast proliferation and inhibits differentiation by interacting with Sirt1 3′UTR to rescue Sirt1 transcriptional suppression by competing with miR-34a ( Wang et al, 2016 ). Similarly, another lncRNA IGF2 AS promotes the proliferation and differentiation of bovine myoblasts by complementing the IGF2 intron and affecting the expression of IGF2 mRNA ( Song et al, 2020 ). In the present study, the sequence of lncSAMM50 is reverse complementary to the upstream region, exon 1, and part of intron 1 of SAMM50 ( Figure 2A ).…”

miR-152 Regulates Bovine Myoblast Proliferation by Targeting KLF6

“…The bovine myoblast cells were isolated from the longissimus or hind limb muscles of fetal bovine samples (Shaanxi Kingbull Livestock, Baoji, China). For detailed isolation methods, please refer to our previous research paper [17]. The isolated cell suspensions were inoculated into Petri dishes and cultured in a high-sugar medium containing 20% FBS and 1% penicillin/streptomycin at 37 • C in 5% CO 2 ; miR-152 mimic and inhibitor were synthesized by RiboBio (Guangzhou, China).…”