LncRNA DDX11-AS1 Promotes Bladder Cancer Occurrence Via Protecting LAMB3 from Downregulation by Sponging miR-2355-5p

Abstract

Background: As a subtype of human genitourinary system cancer, the morbidity of bladder cancer (BC) continues to rise. Because of the high potentiality of cell metastasis, the 5-year survival rate of BC is relatively low. Long noncoding RNAs (lncRNAs) have been verified by a large body of literature to engage in the tumorigenesis of a few cancers. DDX11-AS1 has been elucidated as a malignancy promoter in several cancers; therefore, its mysterious role in BC attracted our interest as being well worth investigating. Aim of the Study: The primary consideration of this article was to clarify the part that DDX11-AS1 plays in the progression of BC. Methods: The expression of DDX11-AS1 in BC was revealed by quantitative real-time polymerase chain reaction. The biological functions of DDX11-AS1 in BC were evaluated through CCK-8 (Cell Counting Kit-8), EDU, TUNEL (TdT-mediated dUTP nick-end labeling), flow cytometry analysis, and Western Blot assays. Luciferase or RNA immunoprecipitation assay was used to investigate the interaction between miR-2355-5p and DDX11-AS1 (or LAMB3). Results: DDX11-AS1 manifested remarkably high level in BC and promoted the malignancy of BC. Moreover, miR-2355-5p was validated to be able to bind with DDX11-AS1 and inhibit cell proliferation in BC. Furthermore, our data suggested that LAMB3 expression was evidently upregulated in BC cells and inversely modulated by miR-2355-5p. Besides, LAMB3 may bind with miR-2355-5p. Ultimately, rescue assays indicated that the restrained development of BC in sh-DDX11-AS1#1-transfected cells could be restored by enforced expression of LAMB3. Conclusion: DDX11-AS1 facilitates the tumorigenesis of BC by the miR-2355-5p/LAMB3 axis.

LncRNA SNHG11 aggravates cell proliferation and migration in triple‑negative breast cancer via sponging miR‑2355‑5p and targeting CBX5

“…The target gene of SNHG11 was predicted and it was determined that miR-2355-5p was a putative target gene of SNHG11. Previous studies have verified that miR-2355-5p has a suppressive effect in various cancers, including chondrosarcoma ( 23 ) and bladder cancer ( 24 ). In our present study, it was determined that miR-2355-5p expression was downregulated in TNBC.…”

“…In recent years, microRNA 2355-5p (miR-2355-5p) has received a lot of attention in some cancers. As reported, miR-2355-5p has been revealed to play a role in the suppression of cancer development in a variety of cancers, including chondrosarcoma ( 23 ) and bladder cancer ( 24 ). However, the interaction between SNHG11 and miR-2355-5p in TNBC has not been elucidated and requires further investigation.…”

LncRNA DDX11 antisense RNA 1 promotes EMT process of esophageal squamous cell carcinoma by sponging miR-30d-5p to regulate SNAI1/ZEB2 expression and Wnt/β-catenin pathway

“…Recent studies have demonstrated that DDX11-AS1, as a novel lncRNA, played an imperative function in the progression and metastasis in multiple cancers, including glioma [ 47 ], bladder cancer [ 21 , 22 ], gastric cancer [ 17 , 18 ], hepatocellular carcinoma [ 15 ], and so on [ 14 , 19 , 20 , 23 ]. In the current research, the DDX11-AS1 functions, as well as molecular mechanisms in EMT process of ESCC, were further explored.…”

Knockdown of long non‑coding RNA DDX11‑AS1 inhibits the proliferation, migration and paclitaxel resistance of breast cancer cells by upregulating microRNA‑497 expression

“…The newly discovered lncRNA DDX11-AS1 has been reported to be abnormally highly expressed in a number of malignant tumors, such as liver cancer ( 26 ), colorectal cancer ( 13 ) and bladder cancer ( 27 ). These findings suggested that DDX11-AS1 may be a tumor marker or therapeutic target.…”