LncRNA GHET1 predicts poor prognosis in hepatocellular carcinoma and promotes cell proliferation by silencing KLF2

Jin, Li; He, Yi; Tang, Shijia; Huang, Shan

doi:10.1002/jcp.26257

Cited by 58 publications

(66 citation statements)

References 31 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,8 Many lncRNAs have been reported in HCC progression. 11 Overexpression of the lncRNA SNHG16 can inhibit HCC proliferation by sponging hsa-miR-93. 11 Overexpression of the lncRNA SNHG16 can inhibit HCC proliferation by sponging hsa-miR-93.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Yin

Zheng

Zhang

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) remains the most common malignant tumor worldwide. Long noncoding RNAs can modulate various tumorigenic processes. In addition, growing evidence has indicated tha the Janus kinase–signal transducer and activator of transcription (JAK‐STAT) pathway is activated in multiple cancers, including HCC. Recently, it was found that LINC00346 can participate in several cancers. Nevertheless, the biological roles of LINC00346 in HCC have been barely investigated. In this study, the function of LINC00346 was specifically concentrated upon. We observed that LINC00346 was obviously elevated in HCC cells (Bel7404, Huh‐6, HepG2, and QGY‐7703 cells). Then, Bel7404 and HepG2 cells were overexpressed with LINC00346. Overexpression of LINC00346 repressed HCC cell survival and cell proliferation. In addition, apoptosis of Bel7404 and HepG2 cells was triggered by LINC00346 upregulation. Bel7404 and HepG2 cell cycle was arrested in the G1 phase by LINC00346. Meanwhile, we conducted wound‐healing assay and Transwell invasion assays. As shown, we observed that the migratory and invasive capacities of Bel7404 and HepG2 cells were remarkably restrained by the increase of LINC00346. Moreover, we showed that LINC00346 overexpression activated the JAK‐STAT3 pathway, which is involved in many cancers. Afterward, in vivo experiments were utilized and we proved that LINC00346 was able to induce HCC tumor growth via activating the JAK‐STAT3 pathway. To conclude, we revealed the potential possibility of developing LINC00346 as an indicator for HCC.

show abstract

Section: Introductionmentioning

confidence: 99%

“…9,10 For example, the lncRNA GHET1 promotes HCC cell proliferation and can predict poor prognosis in HCC via inhibiting KLF2. 11 Overexpression of the lncRNA SNHG16 can inhibit HCC proliferation by sponging hsa-miR-93. 12 In addition, knockdown of ANRIL is able to repress HCC progression through modulating miR-122-5p.…”

Section: Introductionmentioning

confidence: 99%

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Yin

Zheng

Zhang

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…Previous genome-wide screening studies using lncRNA datasets from GEO and TCGA in lung cancer [55,56], gastric cancer [44] and breast cancer [57,58] also identified cell cycle-related lncRNAs. Several studies reported that dysregulation of specific lncRNAs in HCC cell lines caused cell cycle arrest and induced apoptosis, and affected the prognosis of HCC [59,60]. KEGG enrichment analysis revealed a significant enrichment in the PPAR signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Prognostic Long Non-Coding RNA Biomarkers for Predicting Survival in Patients with Hepatocellular Carcinoma

Liao

Yang

Huang

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: The aim of the current study was to identify potential prognostic long non-coding RNA (lncRNA) biomarkers for predicting survival in patients with hepatocellular carcinoma (HCC) using The Cancer Genome Atlas (TCGA) dataset and bioinformatics analysis. Methods: RNA sequencing and clinical data of HCC patients from TCGA were used for prognostic association assessment by univariate Cox analysis. A prognostic signature was built using stepwise multivariable Cox analysis, and a comprehensive analysis was performed to evaluate its prognostic value. The prognostic signature was further evaluated by functional assessment and bioinformatics analysis. Results: Thirteen differentially expressed lncRNAs (DELs) were identified and used to construct a single prognostic signature. Patients with high risk scores showed a significantly increased risk of death (adjusted P < 0.0001, adjusted hazard ratio = 3.522, 95% confidence interval = 2.307–5.376). In the time-dependent receiver operating characteristic analysis, the prognostic signature performed well for HCC survival prediction with an area under curve of 0.809, 0.782 and 0.79 for 1-, 3- and 5-year survival, respectively. Comprehensive survival analysis of the 13-DEL prognostic signature suggested that it serves as an independent factor in HCC, showing a better performance for prognosis prediction than traditional clinical indicators. Functional assessment and bioinformatics analysis suggested that the prognostic signature was associated with the cell cycle and peroxisome proliferator-activated receptor signaling pathway. Conclusions: The novel lncRNA expression signature identified in the present study may be a potential biomarker for predicting the prognosis of HCC patients.

show abstract

“…KLF2 was reported to inhibit colorectal cancer cell proliferation through enhancing HIF‐1α/Notch‐1 signal pathway . It is worthy to note that KLF2 could be downregulated by GHET1 . Therefore, we speculated KLF2 and HIF‐1α/Notch‐1 signal pathway might be involved in the mechanism of GHET1 regulating PC.…”

Section: Introductionmentioning

confidence: 90%

Knockdown of LncRNA GHET1 suppresses prostate cancer cell proliferation by inhibiting HIF‐1α/Notch‐1 signaling pathway via KLF2

Zhu

Tong

Wu³

et al. 2019

BioFactors

View full text Add to dashboard Cite

Prostate cancer (PC) is one of the most common cancers in male groups worldwide. Long noncoding RNAs (LncRNAs) are reported to be dysregulated in a variety of cancers, including PC. This study aimed to explore the role of LncRNA GHET1 in the pathogenesis of PC. RT-qPCR was carried out to examine the relative expression level of GHET1 in PC patients. In vitro, GHET1 siRNA (si-GHET1) was used to investigate the biological role of GHET1 in PC cell lines. Cell proliferation was detected by CCK-8 and colony formation assay, while cell cycle and cell apoptosis were analyzed using flow cytometry. Moreover, western blot was carried out to measure the protein expression levels of KLF2 and HIF-1α/Notch-1 signal pathway. We found that GHET1 showed higher expression in PC tissues and had a negative correlation with KLF2 expression. Knockdown of GHET1 significantly suppressed the cell proliferation, induced cell cycle arrest at G0/G1 phase and promoted cell apoptosis. Additionally, si-GHET1 transfection induced KLF2 upregulation and HIF-1α/Notch-1 signal pathway suppression, which could be rescued by si-KLF2 transfection. These results suggest the key role of GHET1 in PC progression. Moreover, GHET1 might be explored to be a potential target for clinical treatment of PC.

show abstract

LncRNA GHET1 predicts poor prognosis in hepatocellular carcinoma and promotes cell proliferation by silencing KLF2

Cited by 58 publications

References 31 publications

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

LINC00346 promotes hepatocellular carcinoma progression via activating the JAK‐STAT3 signaling pathway

Identification of Potential Prognostic Long Non-Coding RNA Biomarkers for Predicting Survival in Patients with Hepatocellular Carcinoma

Knockdown of LncRNA GHET1 suppresses prostate cancer cell proliferation by inhibiting HIF‐1α/Notch‐1 signaling pathway via KLF2

Contact Info

Product

Resources

About