lncRNA FLVCR1‑AS1 regulates cell proliferation, migration and invasion by sponging miR‑485‑5p in human cholangiocarcinoma

Abstract

Long non-coding RNA (lncRNA) FLVCR1 antisense RNA 1 (FLVCR1-AS1) serves a crucial role in many types of cancer; however, to the best of our knowledge, the biological effect of FLVCR1-AS1 in cholangiocarcinoma (CCA) remains unclear. The present study aimed to elucidate the involvement of FLVCR1-AS1 in the regulation of human CCA cell growth, migration and invasion, as well as the mechanisms underlying its effect. The expression levels of FLVCR1-AS1 in CCA tumor tissues, adjacent normal tissues, CCA cell lines and a cholangiocyte cell line were determined by reverse transcription-quantitative polymerase chain reaction. A significantly higher expression level of FLVCR1-AS1 was identified in CCA tumor tissues and the CCA cell lines HuCCT1 and CCLP1 compared with the normal controls. Short hairpin RNA targeting FLVCR1-AS1 (shFLVCR1-AS1) and a control plasmid (shNC) were transfected into CCA cell lines. Cell proliferation, colony formation, migration and invasion of CCA cells transfected with shFLVCR1-AS1 were significantly suppressed compared with the shNC groups. The expression levels of migration and invasion-associated proteins, including Twist, matrix metalloproteinase (MMP)-2 and MMP-9, were also significantly suppressed by shFLVCR1-AS1-treatment. Furthermore, FLVCR1-AS1 knockdown inhibited tumor growth in a xenograft model. Mechanistically, FLVCR1-AS1 was demonstrated to sponge microRNA-485-5p (miR-485-5p) in human CCA. The expression of miR-458-5p was significantly decreased in CCA tissue compared with normal tissue, and Pearson's correlation analysis revealed that FLVCR1-AS1 expression was negatively correlated with miR-485-5p expression in CCA tissues. These results suggested that lncRNA FLVCR1-AS1 may be used as a novel therapeutic target and a potential diagnostic marker for CCA.

Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

“…It has been reported that in papillary thyroid cancer (PTC), miR-485-5p is sponged by LINC00460 and thereby upregulates Raf1 expression to facilitate PTC progression (46). In addition, in cholangiocarcinoma, miR-485-5p has been demonstrated to negatively regulate FLVCR1-AS1 (47). Furthermore, in non-small cell lung cancer, DGCR5 has been identified to sponge miR-218-5p to promote cancer progression (48).…”

<p>LINC01410 Knockdown Suppresses Cervical Cancer Growth and Invasion via Targeting miR-2467-3p/VOPP1 Axis</p>

“…29 FLVCR1-AS1 sponges miR-485-5p to enhance the growth and metastasis of cholangiocarcinoma. 30 LINC01410 has also been shown to sponge several miRNAs, such as miR-532 and miR-3619-5p. 20,22 Therefore, we analyzed the potential miRNAs of LINC01410 in CC.…”

lncRNA FLVCR1‑AS1 drives colorectal cancer progression via modulation of the miR‑381/RAP2A axis

“…The present study demonstrated that lncRNA FLVCR1-AS1 was elevated in CRC tissues compared with adjacent normal tissues, and the upregulated expression of FLVCR1-AS1 was associated with poor prognosis in patients with CRC, thus implying its essential role in CRC progression. Previous studies have shown that the abnormal expression of FLVCR1-AS1 is associated with the tumorigenesis of multiple cancers (24,25). For example, FLVCR1-AS1 was reported to be upregulated in lung cancer and hepatocellular carcinoma, in which it promoted cell metastasis (26) and tumor growth (27), respectively.…”