Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

Jia, Guanghui; Wang, Yalei; Yu, Yali; Wang, Xiangyu

doi:10.3892/or.2020.7698

Cited by 9 publications

(8 citation statements)

References 48 publications

(53 reference statements)

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“…Similarly, one study reported that miR-495-5p targeted heat shock protein (Hsp90) expression and inactivated Akt1 phosphorylation and blocked PI3K/Akt pathway, resulting in suppression of cell proliferation in osteosarcoma ( 35 ). Another study showed that miR-485-5p can downregulate the expression of baculoviral IAP repeat containing 5 (BIRC5) and block the malignant phenotype of osteosarcoma ( 36 ). In our study, we found that miR-485-5p inhibited the expression of USP22 in osteosarcoma cells, leading to suppression of viability and motility of osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

Long Intergenic Noncoding RNA00265 Enhances Cell Viability and Metastasis via Targeting miR-485-5p/USP22 Axis in Osteosarcoma

et al. 2022

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Osteosarcoma is one of the bone malignancies in children and adolescents. Long noncoding RNAs (lncRNAs) have been demonstrated to participate in osteosarcoma development and progression. Linc00265 has been shown to involve in osteosarcoma oncogenesis; however, the underlying mechanism is largely unclear. In this study, we investigated the function of linc00265 in osteosarcoma cells, including cell viability, migration and invasion. Moreover, we elucidated mechanistically the involvement of linc00265 in osteosarcoma. We found that linc00265 overexpression promoted viability, migration and invasion of osteosarcoma cells. Notably, linc00265 sponged miR-485-5p and increased the expression of USP22, one target of miR-485-5p, in osteosarcoma cells. Strikingly, linc00265 exerted its oncogenic function via regulating miR-485-5p and USP22 in osteosarcoma. Taken together, targeting linc00265 is a promising approach for treating osteosarcoma patients.

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Section: Discussionmentioning

confidence: 99%

Long Intergenic Noncoding RNA00265 Enhances Cell Viability and Metastasis via Targeting miR-485-5p/USP22 Axis in Osteosarcoma

et al. 2022

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“…BIRC5, also known as survivin, is a negative regulatory protein that prevents apoptosis (34) and can regulate cancer development by inhibiting cell apoptosis and inducing cell proliferation (35). The abnormal amplification of BIRC5 has been observed in a variety of malignant tumors, and its overexpression is closely related to tumor occurrence and development, radiotherapy and chemotherapy resistance, and the poor prognosis of cancer patients (36)(37)(38). Recently, BIRC5 has attracted much attention as a molecular target for cancer therapy.…”

Section: Discussionmentioning

confidence: 99%

Establishment and validation of a novel immune-related prognostic signature in malignant pleural mesothelioma

Zhang¹,

Huang²,

Wang³

et al. 2022

Ann Transl Med

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Background: Immune-related genes (IRGs) play an important role in the tumor immune microenvironment and affect tumor prognosis. This study aimed to establish a prognostic signature for malignant pleural mesothelioma (MPM) patients. Methods:We obtained the relevant data of MPM patients in The Cancer Genome Atlas (TCGA), and univariate and multivariate Cox regression were used to construct the prediction signature and verify it with the external validation dataset GSE2549. A nomogram was then constructed, and its predictive ability was evaluated and analyzed the level of immune cell infiltration in different groups in the signature.Results: An IRG-related prognostic signature composed of INHBA, CAT, SORT1, TNFSF13B, and BIRC5 was constructed, with patients divided into high-risk and low-risk groups according to the risk score.The survival time of overall survival (OS), progression-free survival (PFS), disease-free interval (DFI), and relapse-free survival (RFS) in low-risk groups was longer than in high-risk groups. Furthermore, the signature had high predictive performance, and the receiver operating characteristic (ROC) of 1, 2, and 3 years could reach 0.853, 0.881, and 0.914, respectively. The predictive accuracy of the signature was verified by using the independent GSE2549 dataset. The levels of activated CD4 T cells, immature dendritic cells, and type 2 T helper cells were higher in high-risk patients. The gene set enrichment analysis (GSEA) analysis showed that a high concentration and P53 signal pathways were found in high-risk groups.Conclusions: This research developed and verified a new type of immune prognostic signature based on five IRGs, which can predict the prognosis of tumor patients and provide new ideas for individualized treatment.

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“…These tumor-suppressive effects of miR-376b-3p were abolished Two miRNAs, miR-376b-3p and miR-218-5p, were predicted to be down-regulated miRNAs that possibly targeted RGS1. MiR-218-5p was verified as a tumor suppressor in osteosarcoma, while miR-218-5p could possibly be sponged by several long surviving RNAs, including circ_0028171 (26), circSAMD4A (27), and lncRNA NR2F1 (28). Currently, there are no reports on miR-376b-3p in osteosarcoma.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

Zhang¹,

Meng²,

Ma³

et al. 2021

Ann Transl Med

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Background: To investigate the role of microRNA-376b-3p (miR-376b-3p) and regulator of G protein signaling 1 (RGS1) in the proliferation, metastasis, and apoptosis of osteosarcoma.Methods: Differentially expressed genes (DEGs) between tumor and normal tissues from GSE14359 and GSE33382 in the cancer genome atlas (TCGA) dataset were analyzed with GEO2R online. Similarly, differentially expressed miRNAs from GSE70367 were also analyzed with GEO2R. The interaction between the differentially expressed miRNAs and the shared distal metastasis-related DEGs from the two datasets were analyzed using miRWalk and Cytoscape. RGS1 and miR-376-3p were chosen to verify the prediction.RGS1 stably expressing and silencing cells were established based on the MG63 and U2OS cell lines. The targeting of RGS1 with miR-376b-3p was confirmed with Starbase prediction and luciferase reporter assay.Cell proliferation, metastasis, and apoptosis were characterized in vitro and in xenograft mice.Results: A total of 10 up-regulated and 8 down-regulated DEGs were characterized as shared metastasisrelated DEGs for GSE14359 and GSE33382. Among these DEGs, RGS1 was targeted with miR-376b-3p, a predicted down-regulated miRNA in GSE70367. High expression of RGS1 predicted proliferation, invasion, metastases, and poor prognosis in osteosarcoma. Overexpression of RGS1 promoted proliferation, invasion, mobility, and stemness in MG63 and U2OS cells, while silencing of RGS1 had the opposite effect in both cell lines. High expression of RGS1 promoted tumor growth in xenograft nude mice. RGS1 was targeted with miR-376b-3p; the addition of miR-376b-3p down-regulated RGS1, and suppressed cell proliferation, invasion, and metastasis. Meanwhile, sponging of miR-376b-3p had the opposite effect. The suppressive effects of miR-376b-3p could be abolished with RGS1, as cell proliferation, stemness, metastasis, and invasion were all promoted with RGS1 co-transfection in both cell lines.Conclusions: Our study indicated that RGS1 is a tumor-promoting gene in osteosarcoma, which could be inhibited with miR-376b-3p.

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Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

Cited by 9 publications

References 48 publications

Long Intergenic Noncoding RNA00265 Enhances Cell Viability and Metastasis via Targeting miR-485-5p/USP22 Axis in Osteosarcoma

Long Intergenic Noncoding RNA00265 Enhances Cell Viability and Metastasis via Targeting miR-485-5p/USP22 Axis in Osteosarcoma

Establishment and validation of a novel immune-related prognostic signature in malignant pleural mesothelioma

MicroRNA-376b-3p targets RGS1 mRNA to inhibit proliferation, metastasis, and apoptosis in osteosarcoma

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