LncRNA DLEU1 is overexpressed in premature ovarian failure and sponges miR-146b-5p to increase granulosa cell apoptosis

Abstract

Background miR-146b-5p has been reported to participate in premature ovarian failure (POF) in mice. However, its role in POF patients is unclear. We predicted that miR-146b-5p might interact with lncRNA DLEU1, a crucial player in ovarian cancer. We then explored the interaction between DLEU1 and miR-146b-5p. Methods Expression of DLEU1 and miR-146b-5p in POF and control ovary tissues was determined by RT-qPCR. The subcellular location of DLEU1 in human KGN cells was analyzed using subcellular fractionation assays. The direct interaction between DLEU1 and miR-146b-5p was analyzed using RNA pull-down assays. The role of DLEU1 in miR-146a expression was analyzed using overexpression assay. Cell proliferation was analyzed using cell apoptosis assay. Results Increased DLEU1 expression and decreased miR-146b-5p expression were observed in POF. DLEU1 directly interacted with MiR-146b-5p and was expressed in both nuclear and cytoplasm samples of KGN cells. In KGN cells, DLEU1 and miR-146b-5p failed to regulate the expression of each other. However, DLEU1 promoted cell apoptosis and reduced the inhibitory effects of miR-146b-5p on cell apoptosis. Conclusions DLEU1 is overexpressed in POF and sponges miR-146b-5p to increase KGN cell apoptosis.

Human Umbilical Cord Mesenchymal Stem Cells Improve Premature Ovarian Failure through Cell Apoptosis of miR-100-5p/NOX4/NLRP3

“…However, the role of miRNA in follicular development and atresia and its relationship with premature ovarian failure are still unclear. Many miRNAs are highly conserved, and their expression is tissue-specific, reflecting that they have important physiological functions and play an important role in the regulation of cell growth and development, including cell proliferation, differentiation, and apoptosis [ 13 , 14 ]. The experiment elucidated the effect and underlying mechanism of human umbilical cord mesenchymal stem cells (HUCMSCs) in premature ovarian failure.…”

Expression of long noncoding RNAs in the ovarian granulosa cells of women with diminished ovarian reserve using high-throughput sequencing

“…In recent years, several studies have identified an important role for lncRNAs in the regulation of follicle development [ 20 , 21 ]. Studies have identified differential expression of lncRNAs associated with reproductive function by using RNA-seq of OGCs obtained from humans [ 22 ], with most studies focusing on PCOS [ 23 ], endometriosis [ 24 , 25 ], and POF [ 26 , 27 ]. The lncRNAs we identified had fewer exons, shorter ORFs, and shorter transcript lengths compared with those in protein-coding transcripts, and these characteristics were similar to those from porcine OGCs [ 28 ] and human placentas at term [ 29 ].…”

Epigenetic regulation in premature ovarian failure: A literature review

“…One is the interactions between lncRNAs and miRNAs, which may induce ovarian GC apoptosis. For example, Zheng et al found that in POF patients, the lncRNA deleted in lymphocytic leukemia 1 (DLEU1) is upregulated and its overexpression promotes GC apoptosis (Zheng et al, 2021). Zhang et al (Yao et al, 2021).…”