LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR-216a-5p expression

Qu, X.; Shi, You-Ling; Ma, Yan; Bao, Weiwei; Yang, Lei; Li, Jinchao; Zhang, Fan

doi:10.1007/s13577-020-00411-0

Cited by 13 publications

(6 citation statements)

References 33 publications

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“…Most cell nuclei in the PBS‐treated group showed positive staining, indicating active proliferation, whereas the proportion of positively stained nuclei decreased in the other treatment groups (Figure 5H). The findings suggested that T‐miR‐149 had a significant antitumor effect superior to miR‐149 and PYM, thereby confirming the in vitro experimental results 38 . Tumour necrosis factor alpha can directly target specific groups of tumour cells, inducing their apoptosis or necrosis.…”

Section: Resultssupporting

confidence: 77%

Harnessing tetrahedral framework nucleic acids for enhanced delivery of microRNA‐149‐3p: A new frontier in oral squamous cell carcinoma therapy

Xu,

Qin,

Liang

et al. 2024

Cell Proliferation

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Oral squamous cell carcinoma (OSCC), a type of malignant tumour that primarily occurs in the oral mucosa, has drawn considerable attention owing to its aggressive growth and potentially high metastatic rate. Surgical resection is the primary treatment method for OSCC and is typically combined with radiation therapy and chemotherapy. microRNA‐149‐3p (miR‐149) is a negative regulator of the Pi3k/Akt pathway and can effectively inhibit the proliferation of tumour cells. However, the application of miR‐149 is limited owing to its relatively low efficiency of cellular uptake and poor stability when used alone. To overcome these challenges, this study adopted a novel nucleic acid nanostructured material, tetrahedral framework nucleic acids (tFNAs). The use of tFNAs as carriers to assemble the T‐miR‐149 complex reduced the expression of Pi3k and Akt involved in tumorigenesis and alterations in proteins related to cell apoptosis. The results indicated that the bionic drug delivery system has an effective tumour suppressive effect on OSCC in mice, revealing its potential clinical value in the treatment of OSCC.

show abstract

Section: Resultssupporting

confidence: 77%

Harnessing tetrahedral framework nucleic acids for enhanced delivery of microRNA‐149‐3p: A new frontier in oral squamous cell carcinoma therapy

Xu,

Qin,

Liang

et al. 2024

Cell Proliferation

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“…Qu XH et al. showed that miR-216 affects the growth, metastasis, and cell apoptosis of OSCC cells ( 36 ). Sun T et al.…”

Section: Discussionmentioning

confidence: 99%

The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

Lü

Cao

et al. 2022

Front. Oncol.

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HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues and was associated with prognosis. Next, we determined that HOXC-AS3 targeted miR-216 as a sponge and that the F11 receptor (F11R) was the target of miR-216 by online databases analysis, qRT–PCR, and luciferase reporter assay. In addition, the rescue experiments confirmed that HOXC-AS3 regulated the expression of F11R by competitively binding miR-216 and functioning as a competing endogenous RNA (ceRNA). The intracranial glioblastoma mouse model suggested that HOXC-AS3 could promote glioma malignant progression in vivo. In summary, our study shows that the HOXC-AS3/miR-216/F11R axis plays an important role in the malignant progression of glioma, and may provide new ideas for the treatment of glioma.

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“…Wang et al found that lncRNA LACAT1 can promote the malignant progression of OSCC by regulating the activity of miR-4301 [ 24 ]. Qu et al demonstrated that lncRNA DANCR can promote the growth and metastasis of OSCC cells and inhibit the apotosis of OSCC cells by sponging miR-216a-5p [ 25 ]. In this study, we first predicted that there were regulatory sites between miR-149 and GACAT1 through miRanda website, and then verified by dual luciferase reporter gene detection experiment that GACAT1 targeted negative regulation of miR-149.…”

Section: Discussionmentioning

confidence: 99%

LncRNA GACAT1 targeting miRNA-149 regulates the molecular mechanism of proliferation, apoptosis and autophagy of oral squamous cell carcinoma cells

Chen

et al. 2021

Aging

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To explore the effects of lncRNA GACAT1/miR-149 molecular axis on the proliferation, apoptosis, migration and autophagy of oral squamous cell carcinoma (OSCC) cells, and to explore its molecular mechanism. The expressions of lncRNA GACAT1 and miR-149 in tissues and cell lines of patients with OSCC were detected by qRT-PCR. Si-control, GACAT1-siRNA, inhibitor NC and miR-149 inhibitors were transfected into OSCC cells separately or in combination with Lipofectamine 2000. The binding sites between lncRNA GACAT1 and miR-149 were predicted using the miRanda website, and the targeting relationship was verified by dual-luciferase assay. The expression of lncRNA XIST and miR-149 was detected by qRT-PCR. CCK-8 assay was used to detect cell activity. Cell cycle distribution and apoptosis were detected by flow cytometry. Cell migration ability was detected by Transwell assay. The expression of migration and autophagy-related proteins was detected by western blot. LncRNA GACAT1 was highly expressed in cancer tissues and cell lines of OSCC patients ( P < 0.01), while miR-149 was low expressed ( P < 0.01). LncRNA GACAT1 binds to miR-149 targeting. The down-regulation of lncRNA GACAT1 inhibited the proliferation and migration of OSCC cells and promoted apoptosis and autophagy ( P < 0.01). The transfection of miR-149 inhibitor had the opposite effect. Knockdown of lncRNA GACAT1 and transfection with miR-149 inhibitor reversed the effect of GACAT1 silencing on OSCC cells. Inhibition of lncRNA GACAT1 can inhibit the proliferation and migration of OSCC cells, promote apoptosis and autophagy, and the mechanism may be related to the targeting of miR-149.

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LncRNA DANCR regulates the growth and metastasis of oral squamous cell carcinoma cells via altering miR-216a-5p expression

Cited by 13 publications

References 33 publications

Harnessing tetrahedral framework nucleic acids for enhanced delivery of microRNA‐149‐3p: A new frontier in oral squamous cell carcinoma therapy

Harnessing tetrahedral framework nucleic acids for enhanced delivery of microRNA‐149‐3p: A new frontier in oral squamous cell carcinoma therapy

The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

LncRNA GACAT1 targeting miRNA-149 regulates the molecular mechanism of proliferation, apoptosis and autophagy of oral squamous cell carcinoma cells

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