The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression

Abstract

HOXC cluster antisense RNA 3 (HOXC-AS3) is a long noncoding RNA (lncRNA) that plays a crucial role in various tumors; nevertheless, its role in glioma and its mechanism have not been completely elucidated. In this research, we discovered that HOXC-AS3 was over-expression in glioma cells and tissues and was associated with prognosis. Next, we determined that HOXC-AS3 targeted miR-216 as a sponge and that the F11 receptor (F11R) was the target of miR-216 by online databases analysis, qRT–PCR, and luciferase reporter assay. In addition, the rescue experiments confirmed that HOXC-AS3 regulated the expression of F11R by competitively binding miR-216 and functioning as a competing endogenous RNA (ceRNA). The intracranial glioblastoma mouse model suggested that HOXC-AS3 could promote glioma malignant progression in vivo. In summary, our study shows that the HOXC-AS3/miR-216/F11R axis plays an important role in the malignant progression of glioma, and may provide new ideas for the treatment of glioma.

Marsdenia tenacissima extract prevents the malignant progression of glioma through upregulating lncRNA MEG3 and SFRP1‐dependent inhibition of Wnt/β‐catenin pathway

“…Next, according to the intersection of bilateral eyes and ears 2 mm to the right, the skull of nude mice was drilled by using a dental drill. The cell suspensions of U251‐GFP‐Luc (1 × 10 6 cells) were absorbed by a sterilized microsampler and then fixed on the stereotaxic instrument 22,23 . The needle was inserted along the borehole (about 3 mm), and the stereotaxic instrument was started to slowly inject the cell suspension after receding 1 mm.…”

“…The cell suspensions of U251‐GFP‐Luc (1 × 10 6 cells) were absorbed by a sterilized microsampler and then fixed on the stereotaxic instrument. 22 , 23 The needle was inserted along the borehole (about 3 mm), and the stereotaxic instrument was started to slowly inject the cell suspension after receding 1 mm. Post the injection, the drill hole was closed with bone wax, followed by the suturing of skin incision, and the sterilization of wound surface.…”

HOXC Cluster Antisense RNA 3, a Novel Long Non-Coding RNA as an Oncological Biomarker and Therapeutic Target in Human Malignancies

“… 16 Additionally, the expression level of HOXC-AS3 was found to be inversely correlated with the prognosis of glioma patients. 16 Furthermore, HOXC-AS3 could facilitate glioma cell proliferation, migration, invasion, and tumor growth by upregulating F11R expression through sponging miR-216 16 or participate in HOXB13-induced glioblastomas cell proliferation, migration, and invasion. 21 …”

“…HOXC-AS3 displayed different prognostic significance in different cancer types. In most of the reported tumors, including gastric cancer, 17 glioma, 16 breast cancer, 11 , 19 , 20 cervical cancer, 36 hepatocellular carcinoma, 15 HOXC-AS3 overexpression tends to predict poor prognosis, whereas in colorectal cancer (CRC), 12 high expression of HOXC-AS3 indicates good prognosis.…”

Long noncoding RNA HOXC-AS3 remodels lipid metabolism and promotes the proliferation of transformed macrophages in the glioma stem cell microenvironment by regulating the hnRNPA1/CaM axis

“…In GBM, the homeotic box family gene HOXB13 promoted the proliferation, migration and invasiveness of tumour cells through transcriptional upregulation of HOXC-AS3 [ 22 ]. Another study reported that HOXC-AS3 promoted glioma progression by activating miR-216, which regulated F11R expression [ 23 ]. However, the potential mechanism by which HOXC-AS3 regulates the lipid metabolism of macrophages in the GIME has not been reported previously.…”