lncRNA CASC9 sponges miR‑758‑3p to promote proliferation and EMT in bladder cancer by upregulating TGF‑β2

Zeng, Zhen; Chen, Fangfang; Zhan, Hongrui; Chen, Liping; Deng, Qing; Xiong, Tiefu; Li, Yawen; Ye, Jing

doi:10.3892/or.2020.7852

Cited by 27 publications

(25 citation statements)

References 51 publications

(95 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 17 lncRNA CASC9 promoted bladder cancer proliferation and epithelial-mesenchymal transition by sponging miR-758-3p and upregulating TGF-β2. 18 Therefore, it was predicted that miR-671-5p may be a direct target of LINC00908. In this study, LINC00908 was revealed to be a ceRNA by directly targeting miR-671-5p.…”

Section: Discussionmentioning

confidence: 99%

LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

Zeng¹,

Wei²,

Wei³

et al. 2021

CMAR

View full text Add to dashboard Cite

Introduction Emerging evidence has revealed that long noncoding RNA (lncRNA) play important role in almost all kinds of human cancers. LINC00908 has been reported to be involved in the development of prostate cancer, colorectal cancer and gastric cancer which was functioned as an oncogene. However, the potential biology role and molecular mechanism of LINC00908 in diffuse large B-cell lymphoma are still unclear. Methods LINC00908 and miR-671-5p expression were evaluated in DLBCL tissues and cell lines using RT-qPCR. CCK-8 and transwell assay were used to analyze the in vitro role of LINC00908 in DLBCL progression. The xenograft model was used to explore the in vivo role of LINC00908 in DLBCL growth. The physical interaction between LINC00908 and miR-671-5p was confirmed using bioinformatics analysis and a dual luciferase assay, RIP and RNA pull down. Results The expression of LINC00908 was markedly up-regulated in diffuse large B-cell lymphoma tissues and cell lines, and the decreased expression of LINC00908 significantly inhibited diffuse large B-cell lymphoma cell proliferation and invasion. Then, we revealed that LINC00908 directly interacted with miR-671-5p, which was down-regulated in diffuse large B-cell lymphoma cells and highly expressed with LINC00908 knockdown. Moreover, luciferase reporter assays and RNA immunoprecipitation (RIP) assay further proved that miR-671-5p is a direct target of LINC00908 in diffuse large B-cell lymphoma cells. Rescue experiments were also performed, and we confirmed that LINC00908 acts as an oncogene role in diffuse large B-cell lymphoma through miR-671-5p. Finally, the influence of LINC00908 silence significantly inhibited diffuse large B-cell lymphoma growth in vivo. Conclusion LINC00908 promotes malignancy of diffuse large B-cell lymphoma through regulating miR-671-5p.

show abstract

Section: Discussionmentioning

confidence: 99%

LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

Zeng¹,

Wei²,

Wei³

et al. 2021

CMAR

View full text Add to dashboard Cite

show abstract

“…Furthermore, lncRNAs can also regulate the EMT process via some signaling pathways. CASC9 sponges miR‑758‑3p/TGF-β2 (a key gene of the TGF-β signaling pathway) expression to promote proliferation and EMT [ 148 ]. LSINCT5 activates Wnt/β-catenin signaling by interacting with NCYM to promote the EMT process [ 149 ].…”

Section: Emt Processmentioning

confidence: 99%

“…Interestingly, lncRNAs have no relationship with patient sex, while two studies have reported that CASC9 and PlncRNA-1 are associated with patient age. CASC9 upregulation is significantly positively correlated with BCa tumor invasion depth, histological grade, and age; however, sex and tumor volume were not related to CASC9 expression levels [ 62 , 102 , 148 ].…”

Section: Lncrnas Are Associated With Clinicopathological Characteristicsmentioning

confidence: 99%

Biological functions and clinical significance of long noncoding RNAs in bladder cancer

et al. 2021

View full text Add to dashboard Cite

Bladder cancer (BCa) is one of the 10 most common cancers with high morbidity and mortality worldwide. Long noncoding RNAs (lncRNAs), a large class of noncoding RNA transcripts, consist of more than 200 nucleotides and play a significant role in the regulation of molecular interactions and cellular pathways during the occurrence and development of various cancers. In recent years, with the rapid advancement of high-throughput gene sequencing technology, several differentially expressed lncRNAs have been discovered in BCa, and their functions have been proven to have an impact on BCa development, such as cell growth and proliferation, metastasis, epithelial-mesenchymal transition (EMT), angiogenesis, and drug-resistance. Furthermore, evidence suggests that lncRNAs are significantly associated with BCa patients’ clinicopathological characteristics, especially tumor grade, TNM stage, and clinical progression stage. In addition, lncRNAs have the potential to more accurately predict BCa patient prognosis, suggesting their potential as diagnostic and prognostic biomarkers for BCa patients in the future. In this review, we briefly summarize and discuss recent research progress on BCa-associated lncRNAs, while focusing on their biological functions and mechanisms, clinical significance, and targeted therapy in BCa oncogenesis and malignant progression.

show abstract

“…Similarly, the lncRNA tumor suppressor candidate 8 acts as a ceRNA for miR-190b-5p, which modulates the expression of myosin regulatory light chain interacting protein mRNA and inhibits breast cancer metastasis (19). Although these and other studies (20,21) have established lncRNAs as core factors in the development and growth of cancer, little is known regarding the pathophysiological mechanisms of action of lncRNAs in glioma.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA‑324‑5p and upregulating KCTD20 expression

Zhang

Liu

et al. 2021

Oncol Rep

View full text Add to dashboard Cite

Previous studies have demonstrated that long non-coding RNAs (lncRNAs) serve a key role in the development and progression of several types of cancer, including glioma. The lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) contributes to cancer growth through its effects on cell proliferation, migration, invasion and drug resistance. However, the exact regulatory mechanisms via which NEAT1 acts in glioma are unclear. In the present study, the expression levels and function of NEAT1 in glioma tissues and cell lines were examined in vitro and in vivo. By reverse transcription-quantitative PCR and fluorescence in situ hybridization analysis, NEAT1 expression was upregulated in glioma tissues compared with in adjacent normal brain tissues, and elevated NEAT1 levels were associated with poor prognosis. Cell Counting Kit-8, colony formation, ethynyldeoxyuridine, flow cytometry and western blotting assays were performed to detect the effects of NEAT1 on cell biological behavior. Knockdown of NEAT1 in glioma cell lines was associated with cell cycle arrest at the G 0 /G 1 phase, decreased proliferation and elevated apoptosis in vitro, and resulted in reduced tumor growth and increased survival in a mouse xenograft model of glioma. Using bioinformatics analysis, RNA immunoprecipitation experiments and luciferase reporter assays, it was demonstrated that NEAT1 may competitively bind to microRNA (miR)-324-5p, thus blocking its interaction with target mRNAs. Potassium channel tetramerization protein domain containing 20 (KCTD20) was identified as a specific miR-324-5p target. Accordingly, the inhibition of NEAT1 resulted in the downregulation of KCTD20 through competitive binding with miR-324-5p, decreased cell proliferation and increased apoptosis. Concomitant NEAT1 knockdown and inhibition of miR-324-5p partially reversed the effects of NEAT1 knockdown on cell proliferation and apoptosis, and further regulated KCTD20 expression. Collectively, the present findings demonstrated that NEAT1 acted as a competing endogenous RNA for miR-324-5p, and identified the NEAT1/miR-324-5p/KCTD20 axis as a novel regulatory axis and a potential therapeutic target for human glioma.

show abstract

lncRNA CASC9 sponges miR‑758‑3p to promote proliferation and EMT in bladder cancer by upregulating TGF‑β2

Cited by 27 publications

References 51 publications

LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

Biological functions and clinical significance of long noncoding RNAs in bladder cancer

Long non‑coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA‑324‑5p and upregulating KCTD20 expression

Contact Info

Product

Resources

About