LINC00908 Promotes Diffuse Large B-Cell Lymphoma Development by Down-Regulating miR-671-5p

Abstract

Introduction Emerging evidence has revealed that long noncoding RNA (lncRNA) play important role in almost all kinds of human cancers. LINC00908 has been reported to be involved in the development of prostate cancer, colorectal cancer and gastric cancer which was functioned as an oncogene. However, the potential biology role and molecular mechanism of LINC00908 in diffuse large B-cell lymphoma are still unclear. Methods LINC00908 and miR-671-5p expression were evaluated in DLBCL tissues and cell lines using RT-qPCR. CCK-8 and transwell assay were used to analyze the in vitro role of LINC00908 in DLBCL progression. The xenograft model was used to explore the in vivo role of LINC00908 in DLBCL growth. The physical interaction between LINC00908 and miR-671-5p was confirmed using bioinformatics analysis and a dual luciferase assay, RIP and RNA pull down. Results The expression of LINC00908 was markedly up-regulated in diffuse large B-cell lymphoma tissues and cell lines, and the decreased expression of LINC00908 significantly inhibited diffuse large B-cell lymphoma cell proliferation and invasion. Then, we revealed that LINC00908 directly interacted with miR-671-5p, which was down-regulated in diffuse large B-cell lymphoma cells and highly expressed with LINC00908 knockdown. Moreover, luciferase reporter assays and RNA immunoprecipitation (RIP) assay further proved that miR-671-5p is a direct target of LINC00908 in diffuse large B-cell lymphoma cells. Rescue experiments were also performed, and we confirmed that LINC00908 acts as an oncogene role in diffuse large B-cell lymphoma through miR-671-5p. Finally, the influence of LINC00908 silence significantly inhibited diffuse large B-cell lymphoma growth in vivo. Conclusion LINC00908 promotes malignancy of diffuse large B-cell lymphoma through regulating miR-671-5p.

E3 ubiquitin ligase neural precursor cell‐expressed developmentally downregulated gene 4 motivates FOXA1 ubiquitination and restrains proliferation of diffuse large B‐cell lymphoma cells via the Wnt/β‐Catenin pathway

“…Culture of GM12878 cells was with 15% fetal bovine serum (FBS) and 1% Penicillin/ Streptomycin, while DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) in Roswell Park Memorial Institute 1640 medium with 10% FBS and 0.05 mg/mL gentamicin. All cell cultures were presented in an incubator (Zeng et al, 2021). interfering RNA (siRNA) targeting FOXA1 (si-FOXA1), recombinant siRNA (si-NC), was by GenePharma Company.…”

Competitive Endogenous RNA Network Involving miRNA and lncRNA in Non-Hodgkin Lymphoma: Current Advances and Clinical Perspectives

“…Another recently identified oncogenic lncRNA in DLBCL is LINC00908, which, when silenced, results in the inhibition of cell proliferation and invasion. Moreover, LINC00908 function seems to be through directly binding and inhibiting miR-671-5p [ 99 ].…”