LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway

Hsu, Wenchan; Liu, Lifen; Chen, Xin; Zhang, Ying; Zhu, Weipei

doi:10.1186/s40659-019-0240-9

Cited by 49 publications

(34 citation statements)

References 23 publications

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“…KIF18B is another identified oncogene associated with CC cell proliferation and invasion, and loss of KIF18B correlates with decreased cell proliferation and migration, as well as with a loss of β-catenin expression and its target genes [116]. Long noncoding RNA (lncRNA) CASC11 promotes cell proliferation and survival via Wnt/β-catenin signaling [117]. Treatment with DKK1, a negative regulator of the Wnt/β-catenin signaling pathway, leads to decreased cell survival [117].…”

Section: Tumorigenesismentioning

confidence: 99%

Wnt Signaling in Gynecologic Malignancies

McMellen¹,

Woodruff²,

Corr

et al. 2020

IJMS

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Gynecologic malignancies, including ovarian cancer, endometrial cancer, and cervical cancer, affect hundreds of thousands of women worldwide every year. Wnt signaling, specifically Wnt/β-catenin signaling, has been found to play an essential role in many oncogenic processes in gynecologic malignancies, including tumorigenesis, metastasis, recurrence, and chemotherapy resistance. As such, the Wnt/β-catenin signaling pathway has the potential to be a target for effective treatment, improving patient outcomes. In this review, we discuss the evidence supporting the importance of the Wnt signaling pathways in the development, progression, and treatment of gynecologic malignancies.

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Section: Tumorigenesismentioning

confidence: 99%

Wnt Signaling in Gynecologic Malignancies

McMellen¹,

Woodruff²,

Corr

et al. 2020

IJMS

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“…9 Changes in the expression of lncRNAs in cervical cancer have been extensively reported, and have been implicated in carcinogenesis and progression of cervical cancer. For instance, MEG3, 10 STXBP5-AS1, 11 and LINC00052 12 are expressed at low levels in cervical cancer, and suppress malignancy, whereas LUCAT1, 13 LINC00511, 14 and CASC11 15 are upregulated in cervical cancer and play oncogenic roles in the progression of cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>

Wu¹,

Sui²,

Wang³

et al. 2020

OTT

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Purpose: A long noncoding RNA called small nucleolar RNA host gene 7 (SNHG7) is known to be a key regulator of biological processes in multiple human cancer types. In this study, our aims were to determine the expression status of SNHG7 in cervical cancer, to figure out the detailed roles of SNHG7 in cervical cancer cells, and to identify the mechanism underlying the activity of SNHG7 in cervical cancer. Methods: Reverse-transcription quantitative PCR was performed to measure SNHG7 expression in cervical cancer. A Cell Counting Kit-8 assay, flow-cytometric analysis, cell migration and invasion assays, and a tumor xenograft experiment were conducted to respectively determine the effects of SNHG7 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and tumor growth in vivo. Results: SNHG7 was found to be markedly upregulated in cervical cancer tissues and cell lines. Higher SNHG7 expression significantly correlated with FIGO stage, lymph node metastasis, the depth of cervical invasion, and shorter overall survival in patients with cervical cancer. Functional experiments indicated that a SNHG7 knockdown attenuated proliferation, migration, and invasiveness and promoted apoptosis of cervical cancer cells in vitro. The SNHG7 knockdown also slowed tumor growth in vivo. Further investigation showed that SNHG7 acts as a competing endogenous RNA for microRNA-485 (miR-485) in cervical cancer cells, and the inhibitory actions of the SNHG7 knockdown on the malignant phenotype were reversed by miR-485 inhibition. P21-activated kinase 4 (PAK4) was identified as a direct target gene of miR-485 in cervical cancer, and PAK4 expression was promoted by SNHG7. Conclusion: SNHG7 functions as an oncogenic RNA in cervical cancer, competitively binds to miR-485, and thereby upregulates PAK4. This SNHG7-miR-485-PAK4 regulatory network may provide insights into the pathogenesis of cervical cancer, and can help in the identification of novel diagnostic and therapeutic approaches for cervical cancer.

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“…TOP/FOP-Flash luciferase reporter assay was applied to analyze the activity of Wnt/β-catenin signaling pathway [16].…”

Section: Top/fop-flash Luciferase Reporter Analysismentioning

confidence: 99%

LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

Zhang

Huang

et al. 2019

Cancer Cell Int

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Background: Gastric cancer (GC) is one common cancer which occurs in the stomach leading to high mortality around the world. Long non-coding RNAs (lncRNAs) were found overexpressed or silenced in the occurrence and progression of multiple cancers including GC. Method: The gene expression level in GC tissues and cells were analyzed by RT-qPCR. CCK-8, colony formation, flow cytometry and transwell assays were performed for the function analysis of HLA complex group 11 (HCG11). The mechanism study for HCG11 was conducted using RIP, RNA pull down and luciferase reporter assays. Results: HCG11 was discovered highly expressed in GC tissues and cells. Depletion experiments were used to evaluate HCG11 silence on cell proliferation, migration and apoptosis. Moreover, Wnt signaling pathway was found as a tumor promoter in GC. RIP assay, RNA pull down assay and luciferase reporter assay were performed to illustrate the relationship of HCG11, miR-1276 and CTNNB1. Rescue assays revealed that HCG11/miR-1276/CTNNB1 axis regulated the incidence and development of GC. Tumor formation in mice proved that HCG11 was negatively correlated with miR-1276 and had positively correlation with CTNNB1. Conclusion: Overall, HCG11 accelerated proliferation and migration in GC through miR-1276/CTNNB1 and Wnt signaling pathway, revealing that HCG11 could be a brand new target for GC.

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LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway

Cited by 49 publications

References 23 publications

Wnt Signaling in Gynecologic Malignancies

Wnt Signaling in Gynecologic Malignancies

<p>Long Noncoding RNA SNHG7, a Molecular Sponge for microRNA-485, Promotes the Aggressive Behavior of Cervical Cancer by Regulating PAK4</p>

LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

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