LncRNA AFAP1-AS1 contributes to the progression of endometrial carcinoma by regulating miR-545-3p/VEGFA pathway

Zhong, Youwen; Wang, Yidong; Dang, Huimin; Wu, Xiaoling

doi:10.1016/j.mcp.2020.101606

Cited by 22 publications

(18 citation statements)

References 28 publications

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“…MiR-545-3p dysregulation has been reported in diverse cancers. Shi et al reported that miR-545-3p was downregulated in epithelial ovarian cancer (35); Hao et al showed that miR-545-3p inhibits the proliferation and differentiation of osteoblasts (36); and Zhong et al reported that miR-545-3p functions as a tumor suppressor in endometrial carcinoma (37). Furthermore, some studies indicate that miR-545-3p may be the target of circRNAs.…”

Section: Discussionmentioning

confidence: 99%

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The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

et al. 2021

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Glioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. First, qRT-PCR was conducted, which showed that circ_0001367 level was downregulated in glioma tissues and cells. Next, gain-of-function and loss-of-function assays were performed, which indicated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells. Subsequent bioinformatics analysis, dual-luciferase reporter assays, RNA immunoprecipitation assays and cell function assays demonstrated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells by absorbing miR-545-3p and thereby regulating the expression of leucine zipper protein (LUZP1). Finally, an in vivo experiment was conducted, which demonstrated that circ_0001367 inhibited glioma growth in vivo by modulating miR-545-3p and LUZP1. Taken together, the results of this study demonstrate that the circ_0001367/miR-545-3p/LUZP1 axis may be a novel target for glioma therapy.

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Section: Discussionmentioning

confidence: 99%

“…showed that miR-545-3p inhibits the proliferation and differentiation of osteoblasts ( 36 ); and Zhong et al. reported that miR-545-3p functions as a tumor suppressor in endometrial carcinoma ( 37 ). Furthermore, some studies indicate that miR-545-3p may be the target of circRNAs.…”

Section: Discussionmentioning

confidence: 99%

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

et al. 2021

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“…Precious research has shown that miR-545-3p has pivotal roles in the regulating cellular behaviors, such as proliferation, differentiation and migration. 30 , 31 It has recently been identified as a cancer-related miRNA for several cancers. For example, Changjun et al declared that miR-545-3p was highly expressed in hepatocellular carcinoma tissues and cells, and miR-545-3p inhibition limited hepatocellular carcinoma cell proliferation and metastasis via regulating MT1M.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

Mou¹,

Wang²,

Zhang³

et al. 2021

OTT

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Background: Abnormal expression of long noncoding RNAs (lncRNAs) was often involved in tumorigenesis and radiosensitivity of various cancers. The aim of this study was to explore the biological function and regulatory mechanism of lncRNA long intergenic non-protein coding RNA 1410 (LINC01410) in tumorigenesis and radiosensitivity of neuroblastoma (NB). Methods: The expression of LINC01410, microRNA-329-3p (miR-545-3p) and hexokinase 2 (HK2) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methylthiazolyldiphenyl tetrazolium bromide (MTT) assay, colony formation assay and transwell assay were utilized to detect cell viability, colony formation and cell invasion abilities. Glucose consumption or lactate production was measured by glucose assay kit or lactate assay kit, respectively. The interaction between miR-545-3p and LINC01410 or HK2 was predicted by starBase v2.0 and verified by dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assays. Western blot was used to measure the protein expression of HK2. The mice xenograft model was established to investigate the role of LINC01410 in vivo. Results: LINC01410 and HK2 were highly expressed while miR-545-3p was lowly expressed in NB tissues and cells. LINC01410 knockdown inhibited tumorigenesis by repressing cell proliferation and invasion, and increased the radiosensitivity via inhibiting colony formation rates and glycolysis. LINC01410 knockdown also suppressed tumor growth in vivo. Moreover, miR-545-3p could bind to LINC01410 and its downregulation reversed the effects of LINC01410 knockdown on tumorigenesis and radiosensitivity. Additionally, HK2 was a direct target of miR-545-3p and its overexpression attenuated the effects of miR-545-3p restoration on suppression of tumorigenesis and promotion of radiosensitivity. Besides, LINC01410 functioned as a molecular sponge of miR-545-3p to regulate HK2 expression. Conclusion: LINC01410 interference inhibited tumorigenesis and increased radiosensitivity via regulating miR-545-3p/HK2 axis, providing a novel therapeutic strategy for NB.

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“…Also, miR-1271 hampered the malignant behaviors of ECa via binding to LDHA [ 19 ]. A previous research revealed a significant reduction in miR-545-3p in ECa [ 20 ]. However, the connection between circ-IFT80 and miR-545-3p in ECa remains indistinct.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA intraflagellar transport 80 facilitates endometrial cancer progression through modulating miR-545-3p/FAM98A signaling

Wang

Guo

Song³

et al. 2022

J Gynecol Oncol

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Objective Endometrial cancer (ECa) is a common gynecological malignancy. Circular RNAs (circRNAs) have been identified as key regulators of human tumorigenesis and development. Herein, we explored the role and mechanism of circular RNA intraflagellar transport 80 (circ-IFT80, also called circ_0067835) in ECa. Methods Circ-IFT80, microRNA-545-3p (miR-545-3p), and family with sequence similarity 98 member A (FAM98A) were quantified by quantitative real-time polymerase chain reaction or Western blot. The biological characteristics of ECa cells were evaluated via Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, transwell, tube formation and flow cytometry assays. Dual-luciferase reporter assay or RNA pull-down assay was employed to verify the binding relationship between miR-545-3p and circ-IFT80 or FAM98A. Xenograft assays were conducted to analyze the effect of circ-IFT80 in vivo. Results Circ-IFT80 and FAM98A were up-regulated, and miR-545-3p was down-regulated in ECa tissues and cells. Knockdown of circ-IFT80 blocked proliferation, migration, invasion and angiogenesis and promoted apoptosis in ECa cells. Moreover, circ-IFT80 harbored a binding site for miR-545-3p, and the effects of circ-IFT80 were mediated by miR-545-3p. FAM98A was a direct target of miR-545-3p, and miR-545-3p hindered ECa cell progression via targeting FAM98A. Circ-IFT80 induced FAM98A expression through miR-545-3p. Furthermore, silence of circ-IFT80 suppressed tumor growth in vivo. Conclusion Circ-IFT80 may promote the malignant progression of ECa cells at least in part by modulating miR-545-3p/FAM98A axis, providing a potential therapeutic target for ECa.

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LncRNA AFAP1-AS1 contributes to the progression of endometrial carcinoma by regulating miR-545-3p/VEGFA pathway

Cited by 22 publications

References 28 publications

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

Circular RNA intraflagellar transport 80 facilitates endometrial cancer progression through modulating miR-545-3p/FAM98A signaling

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