LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells

Zheng, Yuanyuan; Wang, Zeying; Zhu, Ying; Wang, Wei; Bai, Man; Jiang, Qian; Wang, Yanru; Zhao, Sujun; Yin, Xianbo; Guo, Dan; Bai, Wenlin

doi:10.1080/10495398.2018.1553788

Cited by 24 publications

(18 citation statements)

References 64 publications

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“…We also investigated the expression patterns and functions of lncRNAs, mRNAs and miRNAs to clarify their interactions and relationships with sheep HF development. Several lncRNAs related to hair follicle growth have been reported, such as lnc-000133 and lncRNA-HOTAIR, which participate in hair follicle growth in Cashmere goats ( Jiao et al, 2019 ; Zheng et al, 2019 ), although the specific functions of lncRNA in hair follicle development remain to be fully elucidated. Studies have shown that mRNAs and miRNAs, such as FZD4 , FZD9 , DKK1 , BAMBI , miR-143, and the miR-200 family, play roles in hair follicle development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Involvement of lncRNA–miRNA–mRNA Networks in Hair Follicle Induction in Aohan Fine Wool Sheep Skin

Zhao

Li²,

Liu

et al. 2020

Front. Genet.

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Long non-coding RNAs (lncRNA) and microRNAs (miRNA) are new found classes of non-coding RNAs (ncRNAs) that are not translated into proteins but regulate various cellular and biological processes. In this study, we conducted a transcriptomic analysis of ncRNA and mRNA expression in Aohan fine wool sheep (AFWS) at different growth stages (embryonic day 90, embryonic day 120, and the day of birth), and explored their relationship with wool follicle growth. In total, 461 lncRNAs, 106 miRNAs, and 1,009 mRNAs were found to be differentially expressed during the three stages of wool follicle development. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to clarify the roles of the differentially expressed lncRNA, miRNA, and mRNA in the different stages of wool follicle development. Quantitative real-time PCR (qRT-PCR) was used to validate the results of RNA-seq analysis. lncRNA (MSTRG.223165) was found to act as a competing endogenous RNA (ceRNA) and may participate in wool follicle development by acting as an miR-21 sponge. Network prediction implicated the MSTRG.223165-miR-21- SOX6 axis in the wool follicle development. The targeting relationships of miR-21 with SOX6 and MSTRG.223165 were validated in dual-luciferase assays. This is the first report indicating the association of the lncRNA–miRNA–mRNA network with wool follicle development in AFWS. This study provides new insights into the regulation of the wool follicle growth and represents a solid foundation for wool sheep breeding programs.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Involvement of lncRNA–miRNA–mRNA Networks in Hair Follicle Induction in Aohan Fine Wool Sheep Skin

Zhao

Li²,

Liu

et al. 2020

Front. Genet.

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“…Each type of cashmere goat breed included at least three samples, and all reactions were carried out three times for each sample. The GAPDH gene was used to standardize the expression level of quantitative real-time PCR [14], and to calculate the relative expression using the 2 −ΔΔCt method [25].…”

Section: Methodsmentioning

confidence: 99%

“…Research demonstrated that there were 7000 transcripts that are differentially expressed in cashmere skin during the anagen and telogen phases [13]. Overexpression of lncRNA-000133 resulted in a significant increase in the relative expression of ET-1, SCF, ALP, and LEF1 in dermal papillary cells [14]. Therefore, we speculated that the lncRNAs have a critical role in the fineness of cashmere goat hair.…”

Section: Introductionmentioning

confidence: 99%

An Integrated Analysis of Cashmere Fineness lncRNAs in Cashmere Goats

Zheng

Sheng

Hui

et al. 2019

Genes

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Animal growth and development are regulated by long non-coding RNAs (lncRNAs). However, the functions of lncRNAs in regulating cashmere fineness are poorly understood. To identify the key lncRNAs that are related to cashmere fineness in skin, we have collected skin samples of Liaoning cashmere goats (LCG) and Inner Mongolia cashmere goats (MCG) in the anagen phase, and have performed RNA sequencing (RNA-seq) approach on these samples. The high-throughput sequencing and bioinformatics analyses identified 437 novel lncRNAs, including 93 differentially expressed lncRNAs. We also identified 3,084 differentially expressed messenger RNAs (mRNAs) out of 27,947 mRNAs. Gene ontology (GO) analyses of lncRNAs and target genes in cis show a predominant enrichment of targets that are related to intermediate filament and intermediate filament cytoskeleton. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, sphingolipid metabolism is a significant pathway for lncRNA targets. In addition, this is the first report to reveal the possible lncRNA–mRNA regulatory network for cashmere fineness in cashmere goats. We also found that lncRNA XLOC_008679 and its target gene, KRT35, may be related to cashmere fineness in the anagen phase. The characterization and expression analyses of lncRNAs will facilitate future studies on the potential value of fiber development in LCG.

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“…Additionally, the transcription patterns between resting and growing stages of cashmere goat secondary hair follicles were definituded and found 13 differentially expressed lncRNAs, of which 6 were up-regulated in growth period, and 4 were up-regulated in resting period (Bai et al 2018). The results showed that the expression levels of lncRNA-000133 (Zheng et al 2019), lncRNA-H19 (Zhu et al 2018) and lncRNA-HOTAIR (Jiao et al 2019) in cashmere goat hair follicle development and growth stage were significantly higher than those in resting period. Combined with promoter methylation analysis, the hypermethylation of lncRNA-H19, lncRNA HOTAIR and lncRNA-000133 may be involved in the expression inhibition of Cashmere goat secondary hair follicles.…”

Section: Research Progress Of Non-coding Rna In Hair Follicle Developmentioning

confidence: 92%

On Hair Follicle Development and Wool Production Traits in Sheep: A Review

Wu¹,

Shike²,

Ma³

2021

IJAB

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Hair follicle and skin development is a complex biological process involving many regulatory molecules. Wool trait is a complex quantitative trait controlled by multiple genes and affected by environment. In this paper, the histomorphology of hair follicle development in sheep and the molecular mechanism of hair follicle and wool traits formation were reviewed in order to provide theoretical basis for breeding and selection of sheep wool traits. © 2021 Friends Science Publishers

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LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells

Cited by 24 publications

References 64 publications

Transcriptomic Analysis Reveals the Involvement of lncRNA–miRNA–mRNA Networks in Hair Follicle Induction in Aohan Fine Wool Sheep Skin

Transcriptomic Analysis Reveals the Involvement of lncRNA–miRNA–mRNA Networks in Hair Follicle Induction in Aohan Fine Wool Sheep Skin

An Integrated Analysis of Cashmere Fineness lncRNAs in Cashmere Goats

On Hair Follicle Development and Wool Production Traits in Sheep: A Review

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