lncR26319/miR‐2834/EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori

Abstract

Oocyte maturation is critical for insect reproduction. Vitellogenesis, the timely production and uptake of vitellogenin (Vg), is crucial for female fecundity. Vg is synthesized in fat body and absorbed by the oocytes through endocytosis during insect oogenesis. In the silkworm, Bombyx mori, we discovered that a nucleus-enriched long-noncoding RNA (lncRNA) lncR26319 regulates Endophilin A (EndoA) -a member of the endophilin family of endocytic proteins -through competitive binding to miR-2834. The lncR26319-miR-2834-EndoA axis was required for Vg endocytosis in the silkworm; loss of EndoA or overexpression of miR-2834 significantly reduced egg numbers in virgin moths. In addition, accumulation of miR-2834 resulted in pupal and adult deformation and reduced fecundity in females. The expression of Vg, 30-kDa (30K) protein, and egg-specific protein (Esp) decreased after knockdown of EndoA or overexpression of miR-2834, while knockdown of miR-2834 had an opposite effect on the expression of Vg, 30K protein gene, and Esp. These results suggest that the lncR26319-miR-2834-EndoA axis contributes to the endocytic activity in the Vg uptake and leads to the normal progression of oogenesis in the silkworm. Thus, miR-2834 and EndoA are crucial for female reproduction and could be potential targets for new pest management strategies in lepidopterans.

Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts

“…In insects, ncRNA-involved ceRNA networks were shown to affect and development [ 16 ], metabolism [ 64 ], and immunity [ 65 ]. For example, Wang et al [ 66 ] discovered that the nucleus-enriched lncR26319 in silkworm was able to regulate Endophilin A ( EndoA ), a member of the endophilin family of endocytic proteins, through competitive binding to miR-2834; the lncR26319-miR-2834-EndoA axis was required for Vg endocytosis. Our group previously discovered that 29 up-regulated lncRNAs and 14 up-regulated circRNAs in A. m. ligustica workers’ midguts potentially targeted ame-miR-6001-3p [ 67 , 68 ].…”

Role of small nucleolar RNAs in alternative splicing of the doublesex gene in the silkworm, Bombyx mori

“…The strain Dazao P50 of silkworm B. mori was reared according to the method described by (Wang et al, 2023). Briefly, silkworms were reared on mulberry leaves with a 16:8‐h light‐dark cycle, 25°C temperature, and 60% relative humidity.…”

Juvenile hormone induces reproduction via miR‐1175‐3p in the red imported fire ant, Solenopsis invicta

“…, 2018; Wang et al. , 2023). For instance, knocking out Dicer1 in Drosophila melanogaster can block the miRNA biosynthesis pathway and inhibit oocyte maturation (Nakahara et al.…”

“…They participate in various physiological processes, such as development, cell proliferation and cell death, cell differentiation, and immune responses (Kloosterman & Plasterk, 2006;Dutta et al, 2007;Lindsay, 2008;Bartel, 2009). Many studies have shown the crucial roles of miRNAs in regulating insect growth, development, and reproduction (Lucas et al, 2015;Song et al, 2018;Wang et al, 2023). For instance, knocking out Dicer1 in Drosophila melanogaster can block the miRNA biosynthesis pathway and inhibit oocyte maturation (Nakahara et al, 2005).…”