lnc133b, a novel, long non-coding RNA, regulates bovine skeletal muscle satellite cell proliferation and differentiation by mediating miR-133b

Jin, Cong Fei; Li, Yan; Xiang, Dongxi; Li, Xin; Zhang, Lin Lin; Liu, Xin Feng; Guo, Hong

doi:10.1016/j.gene.2017.07.066

Cited by 30 publications

(25 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although thousands of lncRNAs have been identified and analysed in bovine muscle, several lncRNAs have been reported to play important roles in bovine myoblast proliferation and differentiation (Sun et al 2016;Jin et al 2017;Xu, Ji, et al 2017;Yue et al 2019). Muscle mass and growth rate are two economically important traits in beef production.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterisation of long non-coding RNAs in two Chinese cattle breeds

Jia

Chen

et al. 2020

Italian Journal of Animal Science

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNAs) have been essentially involved in all kinds of important biological processes, such as cell differentiation and development. In this study, we comprehensively profiled genome-wide lncRNAs of bovine skeletal muscle in two Chinese cattle breeds using RNAseq approach. The longissimus dorsi samples were collected from three Charolais (CH) and three Tongjiang (TJ) adult cattle, respectively. Approximately 80 million reads were obtained per library, and by which we assembled 31,009 unique transcripts. We successfully identified a total of 4155 different lncRNAs in six muscle samples, consisting of 3204 intronic lncRNAs, 737 intergenic lncRNAs, 113 sense lncRNAs and 101 antisense lncRNAs. Among them, 32 lncRNAs were differentially expressed (Fold Change >1.0, FDR < 0.05) between CH and TJ. Among these lncRNAs, 1744 identified bovine lncRNAs were located adjacent to 1146 protein-coding genes (<100 kb upstream and downstream) and functionally enriched in organ development, gene regulation and signal transduction. Our results provide a catalogue of bovine skeletal muscle-related lncRNAs, which will complement the list of bovine lncRNAs and contribute to understanding of the molecular mechanism of underpinning muscle development in cattle. HIGHLIGHTS The first study to compare specialised beef cattle breed with Chinese local beef cattle breed on long non-coding RNA (lncRNA). The 4155 different lncRNAs in beef cattle genome were identified to complement the list of bovine lncRNAs. The 1146 protein-coding genes were found maybe in cis-regulatory relationships with lncRNAs to understand the molecular mechanism underpinning muscle development in cattle.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterisation of long non-coding RNAs in two Chinese cattle breeds

Jia

Chen

et al. 2020

Italian Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…Nowadays, the development of biomultiomics is very rapid, which provides us with advantageous methods and technical means for further exploring the mechanism of DNA methylation, histone modification (Bodega et al, 2017;Creyghton et al, 2010;Yan et al, 2016), lncRNAs (Butchart et al, 2016;Guo et al, 2017;Jin et al, 2017), and circRNAs expression in the regulation of muscle formation of bovine skeletal muscle MSCs by SQLE (Cherubini et al, 2019;Legnini et al, 2017). Then, we will use our previous research results, combining with CRISPR/Cas9 technology to produce SQLE gene-edited animals (Hryhorowicz et al, 2017;J and Das, 2019), and verify the regulatory role of SQLE on animal production performance.…”

Section: The Role Of Sqle Gene On Differentiation Of Mscsmentioning

confidence: 99%

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

Zhang¹,

Deng²,

Lv³

et al. 2020

Cellular Reprogramming

View full text Add to dashboard Cite

In this study, Squalene epoxidase (SQLE) overexpression vector was transfected into bovine skeletal musclederived mesenchymal stem/stromal cells (MSCs) to study the molecular mechanism of SQLE regulating meat quality through myogenesis. We initially profiled the expression of SQLE in cattle embryos and adults, in the muscle tissue of four different cattle varieties, and in 11 different tissues/organs of Guangxi cattle variety. Subsequently, we isolated and cultured bovine skeletal muscle-derived MSCs and detected the expression of SQLE during cell proliferation and differentiation. Then, we constructed a bovine SQLE overexpression vector and transfected it into bovine skeletal muscle-derived MSCs by liposome transfection. Cell Counting Kit-8 (CCK-8), 5-ethynyl-20-deoxyuridine (EdU), flow cytometry, immunofluorescence, and quantitative polymerase chain reaction assays were used to characterize cell proliferation and differentiation in detail. The results showed that the relative expression level of bovine SQLE gene in brain tissue was the highest, and in adult muscle tissue was significantly higher than that in embryonic stage. Especially, the expression of SQLE was significantly upregulated in cell differentiation stage. Furthermore, the proliferation, cell cycle, apoptosis, and myoblast differentiation assays indicated that SQLE significantly promoted the differentiation and apoptosis of bovine skeletal muscle-derived MSCs, but inhibited their proliferation. In conclusion, our study reveals the role of SQLE in myoblast differentiation. These results will provide new clues for the regulation network of bovine muscle development.

show abstract

“…Evolution studies have shown that more than 1,000 lncRNAs may have conservative functions in mammals [16]. There were also many reports in bovine indicates lncRNAs function in embryo development [17], skeletal muscle [18] and mammary glands [19]. In mouse, in silico method had been used to identify functional lncRNAs in metabolic process [20].…”

Section: Introductionmentioning

confidence: 99%

LncRNAs Function as Critical Metabolic Regulators in yak Liver by Integrative Transcriptome Analysis

Xia

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The yak (Bos grunniens) is a crucial resource to supply meat and milk to the people localized in Qinghai-Tibetan plateau area. To identify lncRNAs regulating metabolism in yak, this work adopted transcriptome method to simultaneously profile mRNAs and lncRNAs of liver in yak under three representative age (LD: Liver 1 Day, LM: Liver 15 Months, LY: Liver 5 Years) conditions.Result: Of 288 differentially expressed lncRNAs, function-oriented selection yield 88 regulated metabolically related lncRNAs that were differentially expressed at least two age conditions. These lncRNAs predicted by lncRNA-mRNA correlation analysis to function in various aspects of metabolism. Selected regulations of liver metabolically related lncRNAs were further verified by qRT-PCR.Conclusion: Combining high throughput RNA-seq screening screens, bioinformatics predictions, lncRNA-mRNA correlation analysis and qRT-PCR analysis, this study supports that a class of lncRNAs function as important metabolic regulators and establishes a foundation for further investigating the role of lncRNAs in yak.

show abstract

lnc133b, a novel, long non-coding RNA, regulates bovine skeletal muscle satellite cell proliferation and differentiation by mediating miR-133b

Cited by 30 publications

References 32 publications

Genome-wide identification and characterisation of long non-coding RNAs in two Chinese cattle breeds

Genome-wide identification and characterisation of long non-coding RNAs in two Chinese cattle breeds

SQLEPromotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells

LncRNAs Function as Critical Metabolic Regulators in yak Liver by Integrative Transcriptome Analysis

Contact Info

Product

Resources

About