LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition

Singh, Sanjay K.; Koshkin, Alexei A.; Wengel, Jesper; Nielsen, P. H.

doi:10.1039/a708608c

Cited by 708 publications

(593 citation statements)

References 10 publications

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“…2 Moreover, the binding showed high specificity in that a single mismatched base opposite LNA led to a more pronounced drop in denaturation temperature than a mismatch opposite DNA. 9 Nucleic acid duplex formation is generally driven by enthalpy, 40 and disfavoured by entropy.…”

mentioning

confidence: 99%

Locked nucleic acid: tighter is different

Watts

2013

Chem. Commun.

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mentioning

confidence: 99%

Locked nucleic acid: tighter is different

Watts

2013

Chem. Commun.

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“…For each LNA monomer incorporated in the oligonucleotide probe the melting temperature of the corresponding duplex with complementary RNA increases with 3-8C [29]. As a consequence LNAs manifest very high hybridization affinity [30] toward complementary ssRNAs and also excellent single-base-pair mismatch discrimination.…”

Section: Selective Probes For Mirna Detectionmentioning

confidence: 99%

Electrochemical Detection of miRNAs

Lautner

Gyurcsányi

2014

Electroanalysis

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The recent progress made in the development of electrochemical methods for microRNA (miRNA) detection is presented. This progress is conceived to be largely due to the invention of novel assay methodologies and the use of various bioreagents and nanostructures. These enable a rigorous control over the sensing interface, provide enormous signal amplifications and single-base mismatch specificity. Femtomolar or even subfemtomolar detection limits were shown to be feasible by electrochemical assays. Thus electrochemical detection methodologies are of perspective for diagnostic miRNA detection.

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“…1) contains a ribose ring which is locked by a O2'-C4'-methylene linkage, imposing conformational restriction to adopt an N-type sugar puckering. [2][3][4][5]10 Structural investigation of LNA oligonucleotides by NMR spectroscopy revealed their similarities with natural nucleic acid duplexes, and confirmed the RNA mimicking structures adopted by LNA. 11,12 LNA offers key properties needed for successful therapeutic exploitation of oligonucleotides, including (1) unprecedented binding affinity towards RNA (and DNA), (2) excellent base pairing specificity, (3) high bio-stability (resistance towards nucleolytic degradation, (4) low toxicity (at least for many LNA oligonucleotides) in animals and (5) convenient chemistry for manufacturing and modification.…”

Section: Introductionmentioning

confidence: 99%

“…Among the numerous modifications known, LNA has shown broad usefulness within chemical biology. [2][3][4][5][6][7][8][9] LNA: Structural Features and Key Properties LNA ( Fig. 1) contains a ribose ring which is locked by a O2'-C4'-methylene linkage, imposing conformational restriction to adopt an N-type sugar puckering.…”

Section: Introductionmentioning

confidence: 99%