Electrochemical Detection of miRNAs

Lautner, Gergely; Gyurcsányi, Róbert E.

doi:10.1002/elan.201400055

Cited by 40 publications

(16 citation statements)

References 61 publications

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“…In this study, the entire processes were done on the (DOTAP-DOPE) liposomal composition as the sandwich layer in GNP-SCPE 24 . Also to determine the effect of electrochemical changes on DOPE-DOTAP liposomes, each of the liposomes (DOPE and DOTAP), were used on the separate electrodes as control cases.…”

Section: Methodsmentioning

confidence: 99%

“…The initiation and progression of human cancers relies on alterations in miRs expression 2 . Circulating miRNAs seems as package in exosomes that derived from multivesicular bodies, or to be exported in the presence of RNA-binding proteins (i.e., Ago-2) or shed into micro vesicles within membrane blabbing 3 . According to recent literature on this field, circulating miRNAs have diagnostic and prognostic significance in cancer and many other diseases 4 .…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED ARTICLE: Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in The p19 Function

Ghazizadeh

Oskuee

Jaafari

et al. 2018

Sci Rep

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The present study aim to design a liposomal electrochemical sensor using 1, 2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and dioleoylphosphatidylethanolamine(DOPE), chimeric probes and p19, it has been considered as a caliper molecule as well. Also the competitor structural hybrid (RNA) was used to detect three types of miRs in one screen printed electrode modified by gold nanoparticle (SCPE/GNP). In this purpose, the sensor signal stabilized when the cationic DOTAP-DOPE with hybrids of the chimeric probes (Stem, M-linear) sandwiched in order to detect 221–124a miRs. Given the lack of accessibility to RNA-miRs segments of chimeric probes, p19 inhibited the electrochemical reaction and shifted signal to off. After that p19 connected with the free hybrid of T-linear/21miR (just RNA) as competing for structure and the signal was shifted to ON, again. In this study, the electrochemical measurements were performed between the potentials at −0.4 V and +0.4 V with 1 mM [Fe(CN)6]-3-/4 which DOTAP-DOPE acted as an enhancer layer in the electrostatically reaction. This sensor determines as low as 0.4 fM of miRNA with high selectivity and specificity for sequential analysis of 124a-221-21 miRs in just 2 h.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in The p19 Function

Ghazizadeh

Oskuee

Jaafari

et al. 2018

Sci Rep

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“…Thus, many recent attempts at platform development (both uniplex and multiplex) have capitalized on adapting high-sensitivity transducers to novel, selectivity-enhanced recognition elements. As many excellent reviews (25,(61)(62)(63)(64)(65) have provided in-depth background on the various biosensor platforms and techniques, we limit our discussion to recent examples of this trend that utilize direct (unlabeled) miR detection methods.…”

Section: Biosensor Techniquesmentioning

confidence: 98%

MicroRNA Detection: Current Technology and Research Strategies

Hunt

Broyles

Head

et al. 2015

Annual Rev. Anal. Chem.

147

129

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The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques [such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB)] and relatively recent advancements [such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNA/targets] to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.

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“…The concept may be extended to the detection of proteins by using aptamer-based receptors as well as for a wide range of nucleic acids. In this latter case nucleic acid targets of a well-defined length, such as microRNAs [36], are preferred for readily quantifiable resistance changes during nanoparticle displacement assays. While in this study we used impedance spectroscopy to detect changes in the pore resistance, it would probably be feasible to use simple DC measurements instead.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Nanoparticle displacement assay with electrochemical nanopore-based sensors

Lautner

Plesz

Jágerszki³

et al. 2016

Electrochemistry Communications

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Please cite this article as: Gergely Lautner, Mónika Plesz, Gyula Jágerszki, Péter Fürjes, Róbert E. Gyurcsányi, Nanoparticle displacement assay with electrochemical nanopore-based sensors, Electrochemistry Communications (2016Communications ( ), doi: 10.1016Communications ( /j.elecom.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT Abstract:The proof of concept of a nanoparticle displacement assay that enables the use of large diameter nanopores for the detection of targets of smaller molecular dimensions is presented. We hypothesized that an inherent signal amplification should arise from the selective displacement of nanoparticles preloaded in a nanopore by a much smaller molecular target. The method is demonstrated using peptide nucleic acid (PNA)-functionalized gold nanopore arrays in which short DNA-modified gold nanoparticles are anchored by weak interaction. Complementary microRNAs are detected via the resistance change caused by competitive displacement of nanoparticles from the PNA-functionalized nanopores.

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Electrochemical Detection of miRNAs

Cited by 40 publications

References 61 publications

RETRACTED ARTICLE: Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in The p19 Function

RETRACTED ARTICLE: Electrochemical Sensor for Detection of miRs Based on the Differential Effect of Competitive Structures in The p19 Function

MicroRNA Detection: Current Technology and Research Strategies

Nanoparticle displacement assay with electrochemical nanopore-based sensors

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