LLLT actives MMP-2 and increases muscle mechanical resistance after nerve sciatic rat regeneration

Andraus, Rodrigo Antônio Carvalho; Maia, Luciana Prado; Lino, A; Fernandes, Karen Barros Parron; Gomes, Marcos Vinícius de Matos; Guirro, Rinaldo Roberto de Jesus; Barbieri, Cláudio Henrique

doi:10.1007/s10103-017-2169-y

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…The present study showed a significant decrease in SFI value and an increase in values of the electrophysiological and morphological measures in the MLS group compared to the corresponding values in the control group. This result was consistent with those of previous studies that found that laser to be effective in accelerating the regeneration of crushed sciatic nerves in Wistar rats 4,5,11,16,[20][21][22][23][24][25][26][27][28][29] . Laser therapy increases adenosine triphosphate production, nerve metabolism, and myelin production, and enhances axonal sprouting, thereby increasing the proliferation of the nerves after crushing 1,7) .…”

Section: Discussionsupporting

confidence: 93%

“…Low-level laser therapy (LLLT) proved to be a useful modality in promoting the regeneration of peripheral nerves [7][8][9][10] due to its anti-inflammatory and reparative effects 10,11) . Multi-wave locked system (MLS) laser therapy is a recently developed rehabilitation modality that was proven to reduce pain and inflammation in musculoskeletal conditions due to its unique characteristics 12) .…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

et al. 2021

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To investigate the efficacy of the multi-wave locked system laser therapy on the regeneration of peripheral nerve injuries by evaluating the functional, electrophysiological, and morphological changes of the crushed sciatic nerve in Wistar rats. [Materials and Methods] Sixty male Wistar rats (200-250 g) were randomly assigned to control negative, control positive, or laser groups and subjected to no laser therapy or crushing, to crushing without laser therapy, or crushing followed by multi-wave locked system laser therapy five times/week for four weeks (power=1 W, energy density=10 J/cm 2 , total energy=100 J), respectively. Functional, electrophysiological, and morphometric analyses were performed before and 7, 15, 21, and 28 days after crushing. The sciatic functional index, compound motor action potential amplitude, motor nerve conduction velocity, and nerve and myelin sheath diameters were measured.[Results] The sciatic functional index value decreased significantly, while the compound motor action potential amplitude, motor nerve conduction velocity, nerve diameter, and myelin sheath diameter increased significantly in the laser group post-treatment compared to the values in the control groups. [Conclusion] Multi-wave locked system laser therapy was effective in accelerating the regeneration of crushed sciatic nerves in Wistar rats.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

et al. 2021

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“…11 Different experimental studies have used photobiomodulation as a treatment for peripheral nerve injury in rodents to optimize the regeneration of the sciatic nerve after crush injury. 5,8,[12][13][14] The restoration of nerve activity is an effect of photobiomodulation, a physical medium with a prominent level of positive response with 80% efficacy. 12 The effects of photobiomodulation at the cellular level can induce trophic conditions and inhibit the inflammatory processes necessary for nerve regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…5,8,[12][13][14] The restoration of nerve activity is an effect of photobiomodulation, a physical medium with a prominent level of positive response with 80% efficacy. 12 The effects of photobiomodulation at the cellular level can induce trophic conditions and inhibit the inflammatory processes necessary for nerve regeneration. 15 Photobiomodulation improves the production of adenosine triphosphate (ATP), gene expression, and the secretion of neural factors.…”

Section: Introductionmentioning

confidence: 99%

“…5,16 However, conflicting results are frequently observed, most likely due to the significant variation of the parameters used by different researchers, such as the wavelength, irradiation type, doses, and energy density. 5,12,16 Despite a large number of experimental studies in this area of knowledge, there is still a small number of clinical studies reporting these results in humans.…”

Section: Introductionmentioning

confidence: 99%

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Photobiomodulation in Sciatic Nerve Crush Injuries in Rodents: A Systematic Review of the Literature and Perspectives for Clinical Treatment

et al. 2020

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Objective: The aim of the study was to perform a literature review to analyze the effect of photobiomodulation in experimental studies on peripheral nerve regeneration after sciatic nerve crush injury in rodents. Methods: A bibliographic search was performed in the electronic databases, including MEDLINE (PubMed), SCOPUS, and SciELO, from 2008 to 2018. Results: A total of 1912 articles were identified in the search, and only 19 fulfilled all the inclusion criteria. Along with the parameters most found in the manuscripts, the most used wavelengths were 660 nm and 830 nm, power of 30 and 40 mW, and energy density of 4 and 10 J/cm2 . For total energy throughout the intervention period, the lowest energy found with positive effects was 0.70 J, and the highest 1.141 J. Seventeen studies reported positive effects on nerve regeneration. The variables selected to analyze the effect were: Sciatic Functional Index (SFI), Static Sciatic Index (SSI), morphometric, morphological, histological, zymographic, electrophysiological, resistance mechanics and range of motion (ROM). The variety of parameters used in the studies demonstrated that there is yet no pre-determined protocol for treating peripheral nerve regeneration. Only two studies by different authors used the same power, energy density, beam area, and power density. Conclusion: It was concluded that the therapeutic window of the photobiomodulation presents a high variability of parameters with the wavelength (632.8 to 940 nm), power (5 to 170 mW) and energy density (3 to 280 J /cm2 ), promoting nerve regeneration through the expression of cytokines and growth factors that aid in modulating the inflammatory process, improving morphological aspects, restoring the functionality to the animals in a brief period.

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On‐Site Laser Photobiomodulation Treatment of Crushed Muscle Due to Prolonged Pressure in Rats

et al. 2021

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Background and Objectives: Crush injuries and prolonged pressure on muscles lead to bruises and sprains and, in most of the cases, cause distraction of the muscle and release of particles into the blood stream, causing renal and systemic complications in severe cases. Laser photobiomodulation treatment (i.e., laser phototherapy) is a method suggested to decrease the pressure damage in the first 24-48 hours after muscle injury, allowing a faster and more complete physical rehabilitation. We studied the efficacy of non-invasive laser photobiomodulation treatment as an on-site treatment for crush-injured gastrocnemius muscles, developing a moderate muscle crush injury model and aiming at decreasing damage extent while regaining physical competence faster. Study Design/Materials and Methods: Muscle crush injury was performed on 30 female Wistar rats using direct pressure for 10 minutes on the gastrocnemius muscle in both left and right hindlimbs. Immediately after the injury, only the left hindlimb were irradiated for 16 minutes (with 780 nm laser with a power of 250 mW, the energy at the target was 240 J, and the fluence was 1019 J/cm 2 ) for 1, 3, or 7 consecutive days, and sacrificed accordingly. During the follow-up period, 1, 3, or 7 days, both gastrocnemius muscles (of the treated and untreated hindlimbs) were evaluated for electrophysiology and functionality. Results: The laser photobiomodulation treatment showed a significant electrophysiological and functional recovery of the gastrocnemius muscle during the first 3 days after injury, in comparison with the untreated hindlimb. Conclusions: These preliminary results are promising, showing a significant effect of the laser photobiomodulation treatment during the first 3 days after the induction of the muscle crush injury, which is the most critical period in the clinical aspect. These findings suggest a therapeutic approach, which may help restore the muscle after crush injury.

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LLLT actives MMP-2 and increases muscle mechanical resistance after nerve sciatic rat regeneration

Cited by 14 publications

References 26 publications

Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

Efficacy of multi-wave locked system laser therapy on nerve regeneration after crushing in Wister rats

Photobiomodulation in Sciatic Nerve Crush Injuries in Rodents: A Systematic Review of the Literature and Perspectives for Clinical Treatment

On‐Site Laser Photobiomodulation Treatment of Crushed Muscle Due to Prolonged Pressure in Rats

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