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Collonnier, Cécile; Fock, Isabelle; Kashyap, Vandana; Rotino, G. L.; Daunay, Marie‐Christine; Lian, Y.; Mariska, Ika; Rajam, Manchikatla Venkat; Servaes, Aline; Ducreux, G.; Sihachakr, D.

doi:10.1023/a:1010674425536

Cited by 123 publications

(15 citation statements)

References 100 publications

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“…However, higher BAP level (MS4 medium) had negative effect on organogenesis leading to shoots vitrification. Similar results were reported in S. melongena by several authors (Collonier et al, 2001;Kashyap et al, 2005;Sharma, Rajam, 1995). The interaction of NAA, BAP and GA 3 , and also MS0 supplemented with GA 3 resulted in significant shoot elongation throughout stimulation of their growth and development.…”

Section: Discussionsupporting

confidence: 89%

“…Tissue culture protocols for organogenesis, somatic embryogenesis, anther culture and protoplast culture have been well established (Collonier et al, 2001;Kashyap et al, 2003;Khatun et al, 2006;Magioli et al, 2001;Saxena et al, 1992). However, the regeneration efficiency reported in different systems of S. melongena was relatively low (Sharma, Rajam, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Zayova¹,

Vassilevska-Ivanova²,

Kraptchev³

et al. 2012

JCEA

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A protocol for indirect shoot organogenesis of Solanum melongena 'Larga Negra' and 'Black Beauty' was established using hypocotyl and cotyledon derived calluses. The maximum morphogenic callus induction was observed from cultured cotyledons of 30-days old seedlings on Murashige and Skoog (MS) medium containing 2.0 mg/l α-naphthalene acetic acid and 0.5 mg/l 6-benzylaminopurine. The highest percentage of shoot regeneration and the highest mean number of shoots/callus were obtained on hormone-free MS medium. In terms of callus induction and subsequent plant regeneration, cotyledon explants were more responsive than hypocotyl explants. Regenerated shoots (2-3 cm) were rooted on MS hormone-free medium or medium containing 0.1 mg/l indole-3-butyric acid. About 90% of regenerated plantlets survived under field conditions after hardening in the glasshouse. Several somaclones exhibiting useful variation would to be proposed as initial plant material for eggplant breeding programs.Keywords: callus tissue, eggplant, explants, growth regulator, plant regeneration, somaclonal variation Abbreviations: BAP (6-benzylaminopurine), IAA (indole acetic acid), IBA (indole-3-butyric acid), Kn (kinetin), NAA (α-naphthalene acetic acid), PGRs (plant growth regulators), TDZ (thidiazuron), 2,4-D (2,4-Dichlorophenoxyacetic acid) РЕЗЮМЕ Разработена е ефективна система за растителна регенерация чрез индиректен органогенезис при два сорта патладжан (Solanum melongena), която представлява източник на нови форми растения, ценни като изходен материал за селекционни програми. Подробно са описани всички етапи и условия на култивиране, които водят до получаването на растителни регенеранти и успешно развитие в полски уславия.Ключови думи: експланти, калусна тъкан, патладжан, растежен регулатор, регенерация на растенията, сомаклонално вариране 446Journal of Central European Agriculture, 2012, 13(3), p.446-457 DOI: 10.5513/JCEA01/13.3.1070 РАЗШИРЕНО РЕЗЮМЕ Разработен е протокол за индиректен органогенезис при Solanum melongena от калусни култури, получени от два вида експланти (хипокотили и котиледони). В изследването са включени два сорта патладжан -Larga Negra и Black Beauty. Индуциране на морфогенетичен калус е наблюдаван при котиледони от 30-дневни кълнове, култивирани върху среда Murashige and Skoog (MS), съдържаща 2.0 mg/l α-нафтилоцетна киселина и 0.5 mg/l 6-бензиламинопурин. Определени са цвета, консистенцията и структурата на калуса. Преобладаващата част от експлантите формират калус на 14-15 ден от култивирането. Най-висок процент на регенерация и среден брой пъпки от калус са получени на МС среда без фитохормони. Установено е, че отговорът на котиледоните към формиране на калуси и последваща растителна регенерация е по-висок от този на хипокотилите. Растенията-регенеранти са вкоренени на МС среда без растежен регулатор или на среда, съдържаща 0.1 mg/l индолилмаслена киселина. Степента на преживяемост на регенерантите при полски условия е около 90 %. Получени са сомаклонални вариации, които биха могли да бъдат използвани като изх...

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Zayova¹,

Vassilevska-Ivanova²,

Kraptchev³

et al. 2012

JCEA

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“…The regenerated plants were checked for their genetic stability using RAPD primers. Even though numerous protocols for eggplant micropropagation are available, somaclonal variation studies in eggplant are limited (Magioli and Mansur 2005; Collonnier et al 2001). Only a few reports on molecular marker-based analysis of somaclonal variation is available in eggplant (Xing et al 2010).…”

Section: Resultsmentioning

confidence: 99%

“…Only a few reports on molecular marker-based analysis of somaclonal variation is available in eggplant (Xing et al 2010). Molecular markers have been used in eggplant for assessing genetic diversity and varietal differences, and in the construction of genetic linkage maps for the identification of useful agronomic traits (Collonnier et al 2001; Kashyap et al 2003). RAPD has been widely used in genetic variation studies in tissue-culture-derived plants as has been reported in Silybum marianum (L.) (Mahmood et al 2010), Date Palm (Saker et al 2000), and hop ( Humulus lupulus L.) (Patzak 2003).…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation and genetic fidelity study of plant regenerated from inverted hypocotyl explants of eggplant (Solanum melongena L.) cv. Arka Shirish

Mallaya

Ravishankar

2012

3 Biotech

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Genetic variation due to somaclonal variation in micropropagated plants is a beneficial phenomenon for crop improvement. Genetic integrity of the plants derived through micropropagation becomes crucial if genetic transformation studies have to be carried out. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. Hypocotyl explants of Solanummelongena L. cv. Arka Shirish inoculated with inverted polarity in MS media supplemented with 0.5 mg L−1 thidiazuron (TDZ) gave maximum number of shoot buds. Elongation of the shoot buds was achieved on MS medium supplemented with 0.5 mg L−1 2, 3, 5-triiodobenzoic acid (TIBA) and 0.1 mg L−1 gibberellic acid (GA3). The elongated shoots were rooted in MS with 1 mg L−1 indole-3-butyric acid (IBA), and the rooted plants were hardened in the greenhouse. Morphological characteristics were similar in both seed-propagated and micropropagated plants. Random amplified polymorphic DNA analysis carried out with 10 primers for genetic stability studies of the regenerated plants generated 96 scorable bands with a total of 1,056 bands for the primers. Comparison of the bands with the mother plant revealed the monomorphic nature and true-to-type clones. The above regeneration protocol will be useful for micropropagation and genetic transformation studies of S.melongena L. cv. Arka Shirish.

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“…The brinjal or aubergine eggplant, hereafter referred to as eggplant, is cultivated worldwide and is an autogamous diploid with 12 chromosomes (2n = 2x = 24) [5]. Eggplant is susceptible to many bacterial and fungal pathogens and insects, such as the Verticillium dahlia fungus and nematodes [6], which cause significant yield losses. As such, improving resistance to biotic and abiotic stresses is one of the main objectives of eggplant breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of eggplant (Solanum melongena L.) and turkey berry (Solanum torvum Sw.): phylogenomics and disease resistance analysis

Cheng

Deng³

et al. 2014

BMC Genomics

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BackgroundEggplant (Solanum melongena L.) and turkey berry (S. torvum Sw.), a wild ally of eggplant with promising multi-disease resistance traits, are of great economic, medicinal and genetic importance, but genomic resources for these species are lacking. In the present study, we sequenced the transcriptomes of eggplant and turkey berry to accelerate research on these two non-model species.ResultsWe built comprehensive, high-quality de novo transcriptome assemblies of the two Leptostemonum clade Solanum species from short-read RNA-Sequencing data. We obtained 34,174 unigenes for eggplant and 38,185 unigenes for turkey berry. Functional annotations based on sequence similarity to known plant datasets revealed a distribution of functional categories for both species very similar to that of tomato. Comparison of eggplant, turkey berry and another 11 plant proteomes resulted in 276 high-confidence single-copy orthologous groups, reasonable phylogenetic tree inferences and reliable divergence time estimations. From these data, it appears that eggplant and its wild Leptostemonum clade relative turkey berry split from each other in the late Miocene, ~6.66 million years ago, and that Leptostemonum split from the Potatoe clade in the middle Miocene, ~15.75 million years ago. Furthermore, 621 and 815 plant resistance genes were identified in eggplant and turkey berry respectively, indicating the variation of disease resistance genes between them.ConclusionsThis study provides a comprehensive transcriptome resource for two Leptostemonum clade Solanum species and insight into their evolutionary history and biological characteristics. These resources establish a foundation for further investigations of eggplant biology and for agricultural improvement of this important vegetable. More generally, we show that RNA-Seq is a fast, reliable and cost-effective method for assessing genome evolution in non-model species.Electronic supplementary materialThe online version of this article (doi: 10.1186/1471-2164-15-412) contains supplementary material, which is available to authorized users.

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Cited by 123 publications

References 100 publications

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

Indirect Shoot Organogenesis of Eggplant (Solanum Melongena L.)

In vitro propagation and genetic fidelity study of plant regenerated from inverted hypocotyl explants of eggplant (Solanum melongena L.) cv. Arka Shirish

Comparative transcriptome analysis of eggplant (Solanum melongena L.) and turkey berry (Solanum torvum Sw.): phylogenomics and disease resistance analysis

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