Liver tissue trace element levels in HepB patients and the relationship of these elements with histological injury in the liver and with clinical parameters

Şahin, Muammer Melih; Karayakar, Fahri; Erdoğan, Kıvılcım Eren; Baş, Firdevs; Çolak, Tahsin

doi:10.1016/j.jtemb.2017.09.014

Cited by 13 publications

(19 citation statements)

References 24 publications

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“…Overall about 210,000 single measurements (i.e. analysed single spectra) in seven prior found values (11)(12)(13)25). While Cu/S and Cu/Zn are clearly and positively associated, Cu/Fe or Fe/Zn only seem to be weakly associated ( Fig.4a-d).…”

Section: Reference Liver Tissuementioning

confidence: 99%

“…Among histochemical stains for Cu Timm's silver stain seems to be the most sensitive method for the demonstration of copper while Rhodanine and Orcein have been proven to be less sensible in staining either free copper or copper-binding protein corresponding to metallothioneins, respectively (6)(7)(8). For quantitative imaging of metals in tissue electron microprobe (10), laser ablation coupled plasma mass spectroscopy -which is tissue destructive - (11)(12) and micro synchrotron-based X-ray fluorescence (micro-SRXRF) (15)(16)(17)(18)(19) have been introduced.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Osterode

Falkenberg

Ferenci

et al. 2019

Journal of Trace Elements in Medicine and Biology

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Aims of this investigation were to quantify copper (Cu), iron (Fe) and zinc (Zn) along with sulphur (S) and phosphorus (P) in hepatocytes and connective tissue in liver section from patients with Wilson´s disease (WD) by micro Synchrotron X-ray fluorescence (µ-SRXRF). Secondly to establish two-dimensional µ-SRXRF element mappings for comparison with histologically prepared slices, and thirdly to assess whether elemental distributions are associated. Methods: Archival liver tissues from twelve patients with end-stage cirrhosis or fulminant WD were investigated. Mutations in ATP7B have been classified before. For control seven archived normal liver tissues were investigated. µ-SRXRF measurements were performed at the DORIS III storage ring at HASYLAB/DESY (Hamburg, Germany). Two-dimensional element distribution were compared with histologically prepared slices about 20-30µm apart from those investigated by µ-SRXRF. Results: Elementary copper (Cu) could be demonstrated in all investigated liver sections simultaneously with Fe, Zn, P and S. In WD mean Cu was 20 fold increased in hepatocytes and threefold in fibrotic areas in comparison with controls. In regeneration nodules Cu was strikingly inhomogeneous distributed. Cu concentrations measured by µ-SRXRF correlated with those measured by atom absorption spectroscopy. Strong associations in their regional distribution existed between Zn and Cu or Fe and S. Moreover, differences in Cu/S were found between hepatocytes and fibrotic areas. An increase of Fe could only be documented in hepatocytes compared to fibrotic areas. With a beam size of 15x15µm two-dimensional distributions of these elements are morphologically comparable with histological section with a magnification of about 25x optic microscope. Conclusions: µ-SRXRF investigations are a valuable tool for quantifying element concentrations in biological tissues and further provide 2-dimensional information of element distribution and elemental association in a biological tissues, thus speeding up basic knowledge in a synopsis with biological and clinical data.

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Section: Reference Liver Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Osterode

Falkenberg

Ferenci

et al. 2019

Journal of Trace Elements in Medicine and Biology

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“…As stated above, for ICP-based techniques, total decomposition of the biological sample matrix, i.e., introduction of a clear solution, is a prerequisite for reproducible and valid trace quantification. Therefore, sample pre-treatment yields at total digestion and is often performed using aggressive acid mixtures for several hours at elevated temperature and/or by microwave assistance [ 10 – 12 , 14 , 15 ]. Furthermore, the investigated sample amounts are, due to practical reasons of sample preparation, rather high in comparison with the minute sample amounts applied in this work.…”

Section: Resultsmentioning

confidence: 99%

“…These authors observed systematic minor recoveries in the suspensions of the real samples, which were then corrected using the obtained factors. In this work, a different preparation approach was followed and fresh real samples were partly decomposed by addition of nitric acid in order to [10] homogenize the samples. Here, validation of Fe quantification in tissues as well as in cells was achieved over a broad concentration range using GFAAS as a reference method.…”

Section: Analytical Figures Of Merit and Comparison With Other Methodsmentioning

confidence: 99%

“…This technique can also be applied to suspensions, slurries, and even solids; however, it is restricted to the determination of single elements and requires external calibration. Alternatively, inductively coupled plasma-mass spectrometry (ICP-MS) or inductively coupled plasma-optical emission spectrometry (ICP-OES) was suggested [10][11][12][13][14][15][16][17]. These techniques provide multi-element determination; however, complete dissolution of the samples is required, involving therefore time-and reagent-consuming sample preparation [18].…”

Section: Introductionmentioning

confidence: 99%

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Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

et al. 2020

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Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow-derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material "bovine liver" (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92-106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range. Keywords Total reflection X-ray fluorescence spectrometry. Iron trace analysis. Biometal trace analysis. Bone marrow-derived macrophages. Liver cells. Liver tissues Published in the topical collection featuring Female Role Models in Analytical Chemistry.

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Relationship between Trace Elements and Matrix Metalloproteinases 2 and 9 and their Tissue Inhibitors in Medullary Thyroid Carcinoma

Vázquez-Lorente

Dundjerović

Tatic

et al. 2022

Biol Trace Elem Res

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Medullary Thyroid Carcinoma (MTC) constitutes around 5% of all thyroid cancers. Trace elements assessment has emerged as a useful strategy in the diagnostics of MTC combined with Matrix Metalloproteinases (MMPs) and Tissue Inhibitors of Matrix Metalloproteinases (TIMPs) analysis. The aim of this study was to compare the presence and content of trace elements (i.e., Copper (Cu), Zinc (Zn), Iron (Fe), and Manganese (Mn)) in MTC with respect to control samples and their potential relationship with markers of MTC in tissues. The study included 26 patients who had undergone thyroidectomy, due to the diagnosis of MTC and 17 patients as control. We combined tumour pathology and staging, immunohistochemical analysis of calcitonin, MMPs, and TIMPs, with analytical biochemistry using Inductively Coupled Plasma – Mass Spectrometry (ICP-MS) to determine the levels of trace elements. No differences by MTC type for MMPs and their TIPMs, although strong TIMP-1 and TIMP-2 immunohistochemical expression of MTC were unveiled. Additionally, Zn, Fe, and Mn tended to be decreased, and Cu to be increased in samples presenting MTC with respect to controls. Moreover, Zn was the unique trace element which seemed to be correlated with MMPs and TIMPs. Trace elements such as Zn, Fe, and Mn are decreased in tissues affected by MTC. In addition, Zn may be the trace element which saves more relationship with the proportion and intensity of MMPs, being considered altogether useful biomarkers of MTC. We therefore suggest the analysis of novel and traditional markers of MTC as a novel approach in this pathology.

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Liver tissue trace element levels in HepB patients and the relationship of these elements with histological injury in the liver and with clinical parameters

Cited by 13 publications

References 24 publications

Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

Relationship between Trace Elements and Matrix Metalloproteinases 2 and 9 and their Tissue Inhibitors in Medullary Thyroid Carcinoma

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