Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Osterode, W.; Falkenberg, Gerald; Ferenci, Péter; Wrba, Fritz

doi:10.1016/j.jtemb.2018.09.007

Cited by 9 publications

(12 citation statements)

References 30 publications

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“…The latter can be achieved using e.g. laser-ablation inductively coupled mass spectrometry (LA-ICP-MS) or micro-X-ray fluorescence spectrometry (μ-XRF), which has been shown for other biological tissues recently [ 8 , 28 – 30 ]. However, these techniques require time-consuming sample preparation and stabilization of tissue as well as sophisticated calibration strategies for quantification.…”

Section: Introductionmentioning

confidence: 99%

Determination of trace elements in placenta by total reflection X-ray fluorescence spectrometry: effects of sampling and sample preparation

Hauser

Andres²,

Leopold

2022

Anal Bioanal Chem

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Placental elemental composition can serve as an indicator for neonatal health. Medical studies aiming at revealing such cause-and-effect relationships or studies monitoring potential environmental influences consist of large sample series to ensure statistically sufficient data. Several analytical techniques have been used to study trace metals in human placenta. However, most techniques require provision of clear liquid sample solutions and therefore time- and reagent-consuming total digestion of biological tissue is necessary. In total reflection X-ray fluorescence spectrometry (TXRF)—a straightforward multielement analytical technique—in contrast suspensions of minute sample amounts can be analyzed directly. Therefore, herein we report on a valid method to prepare homogenous sample suspensions for sustainable and fast TXRF analysis of large sample series. The optimized method requires only 10 mg of powdered placental tissue and 1 mL nitric acid. Suspensions are readily prepared within 30 min and the found mass fractions of major, minor, and trace elements are in good agreement in comparison to analysis of digests. In addition, possible effects on fixation time and the exact sampling location, i.e., maternal vs. fetal side of the placenta, were studied applying this method. Thereby, significant differences for fetal placenta tissue compared to maternal or intermediate tissue were observed revealing accumulation of trace elements in the fetal side of the placenta. Furthermore, considerable depletion of up to 60% mass fraction with longer fixation duration occurred in particular in fetal placenta tissue. These findings help to understand the large ranges of mass fraction of elements in placenta reported in the literature and at the same time indicate the necessity for more systematic investigation of non-homogenous elements distributed in placenta taking sampling and stabilization methods into account. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Determination of trace elements in placenta by total reflection X-ray fluorescence spectrometry: effects of sampling and sample preparation

Hauser

Andres²,

Leopold

2022

Anal Bioanal Chem

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“…This sensitive and non-destructive method of multi-elemental analysis offers low detection limits and a short acquisition time. The above-mentioned properties enable the use of the analytical technique to investigate the elemental topography of biomedical samples [ 8 , 9 , 10 , 11 ]. As a result of the SR-XRF measurements, we obtained two-dimensional maps of P, S, K, Ca, Fe, Cu, Zn and Se distribution.…”

Section: Introductionmentioning

confidence: 99%

Altered Elemental Distribution in Male Rat Brain Tissue as a Predictor of Glioblastoma Multiforme Growth—Studies Using SR-XRF Microscopy

Planeta

Setkowicz

Czyzycki

et al. 2022

IJMS

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Glioblastoma multiforme (GBM) is a particularly malignant primary brain tumor. Despite enormous advances in the surgical treatment of cancer, radio- and chemotherapy, the average survival of patients suffering from this cancer does not usually exceed several months. For obvious ethical reasons, the search and testing of the new drugs and therapies of GBM cannot be carried out on humans, and for this purpose, animal models of the disease are most often used. However, to assess the efficacy and safety of the therapy basing on these models, a deep knowledge of the pathological changes associated with tumor development in the animal brain is necessary. Therefore, as part of our study, the synchrotron radiation-based X-ray fluorescence microscopy was applied for multi-elemental micro-imaging of the rat brain in which glioblastoma develops. Elemental changes occurring in animals after the implantation of two human glioma cell lines as well as the cells taken directly from a patient suffering from GBM were compared. Both the extent and intensity of elemental changes strongly correlated with the regions of glioma growth. The obtained results showed that the observation of elemental anomalies accompanying tumor development within an animal’s brain might facilitate our understanding of the pathogenesis and progress of GBM and also determine potential biomarkers of its extension. The tumors appearing in a rat’s brain were characterized by an increased accumulation of Fe and Se, whilst the tissue directly surrounding the tumor presented a higher accumulation of Cu. Furthermore, the results of the study allow us to consider Se as a potential elemental marker of GBM progression.

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“…Fe-positive granules in cardiomyocytes ↓ serum Fe [90][91][92][93] ↑ Fe in dentate nucleus, red nucleus [94,95] Fe switched from oligodendroglia to microglia in dentate nucleus [96][97][98] Fe presumably primarily accumulated in mitochondria [99,100] Wilson Disease ↑ Fe in liver ↑ serum Fe, ferritin, hepcidin, soluble transferrin receptor [101][102][103] ↑ Fe in globus pallidus, putamen, caudate, thalamus, substantia nigra, red nucleus, subthalamic nucleus [104,105] ↑ Fe in excessive macrophages, astrocytes [106] n.a.…”

Section: Friedreich Ataxiamentioning

confidence: 99%

“…Additionally, increased serum IL-1 and TNF-alpha was observed in patients with the most severe brain MRI abnormalities [270]. Increased Fe concentration was observed also in the liver, particularly in hepatocytes [102,103,271,272]. Serum ferritin was found to be an independent predictor of liver injury in WD, similarly to other liver disorders [273].…”

Section: Other Genetic Disorders With Brain Iron Depositsmentioning

confidence: 99%

Cerebral Iron Deposition in Neurodegeneration

et al. 2022

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Disruption of cerebral iron regulation appears to have a role in aging and in the pathogenesis of various neurodegenerative disorders. Possible unfavorable impacts of iron accumulation include reactive oxygen species generation, induction of ferroptosis, and acceleration of inflammatory changes. Whole-brain iron-sensitive magnetic resonance imaging (MRI) techniques allow the examination of macroscopic patterns of brain iron deposits in vivo, while modern analytical methods ex vivo enable the determination of metal-specific content inside individual cell-types, sometimes also within specific cellular compartments. The present review summarizes the whole brain, cellular, and subcellular patterns of iron accumulation in neurodegenerative diseases of genetic and sporadic origin. We also provide an update on mechanisms, biomarkers, and effects of brain iron accumulation in these disorders, focusing on recent publications. In Parkinson’s disease, Friedreich’s disease, and several disorders within the neurodegeneration with brain iron accumulation group, there is a focal siderosis, typically in regions with the most pronounced neuropathological changes. The second group of disorders including multiple sclerosis, Alzheimer’s disease, and amyotrophic lateral sclerosis shows iron accumulation in the globus pallidus, caudate, and putamen, and in specific cortical regions. Yet, other disorders such as aceruloplasminemia, neuroferritinopathy, or Wilson disease manifest with diffuse iron accumulation in the deep gray matter in a pattern comparable to or even more extensive than that observed during normal aging. On the microscopic level, brain iron deposits are present mostly in dystrophic microglia variably accompanied by iron-laden macrophages and in astrocytes, implicating a role of inflammatory changes and blood–brain barrier disturbance in iron accumulation. Options and potential benefits of iron reducing strategies in neurodegeneration are discussed. Future research investigating whether genetic predispositions play a role in brain Fe accumulation is necessary. If confirmed, the prevention of further brain Fe uptake in individuals at risk may be key for preventing neurodegenerative disorders.

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Quantitative trace element mapping in liver tissue from patients with Wilson`s disease determined by micro X-ray fluorescence

Cited by 9 publications

References 30 publications

Determination of trace elements in placenta by total reflection X-ray fluorescence spectrometry: effects of sampling and sample preparation

Determination of trace elements in placenta by total reflection X-ray fluorescence spectrometry: effects of sampling and sample preparation

Altered Elemental Distribution in Male Rat Brain Tissue as a Predictor of Glioblastoma Multiforme Growth—Studies Using SR-XRF Microscopy

Cerebral Iron Deposition in Neurodegeneration

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