Liver tissue engineering using functional marine biomaterials

Jiang, Tao; Singh, Bijay; Choi, Yun‐Jaie; Akaike, Toshihiro; Cho, C.-S.

doi:10.1016/b978-1-78242-086-6.00006-6

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…As a deacetylated derivative of chitin, chitosan (Cs) is composed of β-(1-4) linked D-glucosamine and N-acetylglucosamine groups, the distribution of which varies with the degree of deacetylation (Jiang et al, 2015;Ahmed et al, 2018;Islam et al, 2020). Since chitosan mimics glycosaminoglycan, the foundational element of the ECM, it has been used as a scaffold for tissue engineering in recent years (Ardeshirylajimi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Synthesis, optimization, and cell response investigations of natural-based, thermoresponsive, injectable hydrogel: An attitude for 3D hepatocyte encapsulation and cell therapy

Gholami

Tajabadi

Khavandi

et al. 2023

Front. Bioeng. Biotechnol.

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For the purpose of developing a 3D vehicle for the delivery of hepatocytes in cell therapy, the improved system of crosslinker and new gelling agent combinations consisting of glycerophosphate and sodium hydrogen carbonate have been employed to produce injectable, thermoresponsive hydrogels based on chitosan and silk fibroin. Adjusting the polymer-to-gelling agent ratio and utilizing a chemical crosslinker developed hydrogel scaffolds with optimal gelling time and pH. Applying sodium hydrogen carbonate neutralizes chitosan while keeping its thermoresponsive characteristics and decreases glycerophosphate from 60% to 30%. Genipin boosts the mechanical properties of hydrogel without affecting the gel time. Due to their stable microstructure and lower amine availability, genipin-containing materials have a low swelling ratio, around six compared to eight for those without genipin. Hydrogels that are crosslinked degrade about half as fast as those that are not. The slowerr degradation of Silk fibroin compared to chitosan makes it an efficient degradation inhibitor in silk-containing formulations. All of the optimized samples showed less than 5% hemolytic activity, indicating that they lacked hemolytic characteristics. The acceptable cell viability in crosslinked hydrogels ranges from 72% to 91% due to the decreasing total salt concentration, which protects cells from hyperosmolality. The pH of hydrogels and their interstitial pores kept most encapsulated cells alive and functioning for 24 h. Urea levels are higher in the encapsulation condition compared to HepG2 cultivated alone, and this may be due to cell-matrix interactions that boost liver-specific activity. Urea synthesis in genipin crosslinked hydrogels increased dramatically from day 1 (about 4 mg dl−1) to day 3 (approximately 6 mg dl−1), suggesting the enormous potential of these hydrogels for cell milieu preparation. All mentioned findings represent that the optimized system may be a promising candidate for liver regeneration.

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Section: Introductionmentioning

confidence: 99%