Liver reconstruction on the chorioallantoic membrane of the chick embryo

Chiba, Akina; Yui, Chisato; Hirano, Shigeki

doi:10.1679/aohc.73.45

Cited by 9 publications

(9 citation statements)

References 20 publications

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“…The establishment of the windowing method has led to its application in various fields of research, including studies in embryology and the mechanism of malformations ( Matheus et al , 2019 ), imaging ( Kulesa et al , 2010 ; Funahashi et al , 2014 ), embryo manipulations such as xenotransplantation ( Boulland et al , 2010 ), chorioallantoic membrane assays ( Ribatti, 2016 ; Vu et al , 2018 ), evaluation of pluripotency in stem cells ( Haraguchi et al , 2016 ), and basic research in regenerative medicine ( Chiba et al , 2010 ). However, although methodologies that use the windowing approach and surrogate eggshell approach managed to continue embryonic development after various treatments, it was difficult to observe the processes of development.…”

Section: Ex Ovo Culture Systemmentioning

confidence: 99%

<i>Ex Ovo</i> Culture System for Avian Embryos and its Application

Tahara

Obara²

2021

J. Poult. Sci.

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Ex ovo culture of avian embryos can be applied not only to embryology but also to various fields of basic research such as embryo manipulation, toxicology, and regenerative medicine. The windowing method, which facilitates various manipulations and observations by opening a hole in one part of the eggshell, and culture systems using surrogate eggshells, are widely used. Despite this, biology lessons in high schools cover shell-less culture systems, which involve the development of avian embryos in artificial vessels, such as rice bowls, without using surrogate eggshells. However, as embryo development stops at its early stages in this method, it is not possible to continuously observe the development of the embryo. This led to attempts to develop an embryo culture method using a complete artificial culture vessel that does not use surrogate eggshells, and Kamihira et al. (1998) succeeded in hatching quail embryos in an artificial culture vessel using polytetrafluoroethylene membranes. In addition, Tahara succeeded in hatching chick embryos in artificial culture vessels that used cling film made of polymethylpentene and reported their detailed methodology ( Tahara and Obara, 2014 ). These technologies are being applied not only to school education but also to various fields of research.

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Section: Ex Ovo Culture Systemmentioning

confidence: 99%

<i>Ex Ovo</i> Culture System for Avian Embryos and its Application

Tahara

Obara²

2021

J. Poult. Sci.

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“…Already in 1916, for the first time, multiple chicken tissues were grafted in the chicken embryos [193]. More recently, allogeneic and xenogeneic tissues were successfully grafted on the CAM, such as liver [194], proliferative endometrium [195], adrenal gland or cerebellum [196], or intact human skin [58,179], see Table 8.…”

Section: Cam As a Screening Platformmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and / or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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“…Due to the lack of a fully developed immune system in the chick embryo 3 , 5 , the CAM is suitable for tumor engraftment to study various aspects of the angiogenic and metastatic potential of human malignancies 3 , 4 , such as glioma 6 - 8 , colorectal cancer 9 , 10 , leukemia 11 , ovarian cancer 4 , prostate cancer 12 , 13 , and osteosarcoma 14 . In addition, the CAM has been used to test the biocompatibility of organic and inorganic materials 15 - 17 , the ability of bacterial strains to invade epithelial barriers 18 , vascular anomalies and Moyamoya Disease 19 - 24 , the organogenetic capacity of xenograft or homograft 25 , 26 , the mechanisms of wound healing 27 , 28 , and the differentiation of the blood-brain barrier 29 , 30 . The aim of the present review is to summarize the application of the CAM in the field of neurosurgery research.…”

Section: Introductionmentioning

confidence: 99%

Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease

Yuan¹,

Xu²,

Wu³

et al. 2014

Int. J. Med. Sci.

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The chick embryo chorioallantoic membrane (CAM) is a highly vascularized extraembryonic membrane. Because of its ease of accessibility, extensive vascularization and immunodeficient environment, the CAM has been broadly used in the oncology, biology, pharmacy, and tissue regeneration research. The present review summarizes the application of the CAM in neurosurgery disease research. We focused on the use of the CAM as an assay for the research of glioma, vascular anomalies, Moyamoya Disease, and the blood-brain barrier.

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Liver reconstruction on the chorioallantoic membrane of the chick embryo

Abstract: o f s i n u s o i d a l s p a c e s . W e t h e r e f o r e c o n s i d e r t h a t hematopoiesis is important for liver regeneration with a normal structure. Transplantation of the liver in the chorioallantoic membrane could be also useful for research into liver regeneration.

Cited by 9 publications

References 20 publications

<i>Ex Ovo</i> Culture System for Avian Embryos and its Application

<i>Ex Ovo</i> Culture System for Avian Embryos and its Application

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease

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