Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease

Yuan, Yongjie; Xu, Kan; Wu, Wei; Luo, Qi; Yu, Jinlu

doi:10.7150/ijms.10443

Cited by 31 publications

(24 citation statements)

References 68 publications

(97 reference statements)

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“…The CAM is an extraembryonic membrane whose main function is to ensure the exchange of gases and nutrients. Because of its large vascularity and ease of use, CAM is a popular research tool widely used to study glioma angiogenesis, growth, invasiveness and screening of anti-tumor drugs [28]. Therefore, according to the results, the aqueous extract of Lophira procera may have good inhibitory activity on tumor growth by blocking angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon

Misso

Sima-Obiang

Ndong

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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The objective of this study was to perform phytochemical screening, to determine the content of phenolic compound, to evaluate antioxidant, anti-inflammatory and antiangiogenic activities of ethanol, waterethanol and water extracts of Lophira procera. Antioxidant activity was determined by 2,2-diphenyl-1picrylhydrazyl (DPPH) and phosphomolybdenum assay, anti-inflammatory activity by proteins denaturation inhibition and membranes stabilization test and antiangiogenic activity by chicken chorioallantoic membrane (CAM) method. The results showed that this plant is rich in saponins, polyphenols, tannins, total flavonoids, proanthocyanidins and coumarins. Extracts presented a strong antioxidant activity (IC 50 values of 5.452 ± 0.119 lg/mL and 6.346 ± 0.544 lg/mL and respective AAI of 9.173 ± 0.203 and 7. 919 ± 0.711). Excellent anti-inflammatory activity was also observed (IC 50 = 16.952 ± 1.897 and IC 50 = 2 3.172 ± 0.066 lg/mL for inhibition of protein denaturation and membrane stabilization respectively). Finally, extracts manifested a very good anti-angiogenic activity (with inhibitions ranging from 57.142 ± 0.124% to 100%). These biological activities are certainly due to high content of phenolic compound. This is the first study to report the phytochemical screening, the content of phenolic compound, the antioxidant, anti-inflammatory and antiangiogenic activities of extract derived from Lophira procera. The use of this plant in traditional medicine against ulcers, breast cancer, kidney and dental pain is therefore justified and its potential as a candidate for bioactive therapeutic molecule.

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Section: Discussionmentioning

confidence: 99%

Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon

Misso

Sima-Obiang

Ndong

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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“…Indeed, as we identify similarities between all vertebrates from molecular to organismal levels, the use of the bird embryo continues to expand, including animal modeling for areas as diverse as nonclinical safety studies of pharmaceuticals and vaccines (Saw et al, 2008;Bjornstad et al, 2015) to toxicology (Kopf and Walker, 2009;Smith et al, 2012) to prototyping surgical and measurement apparatus (Filas et al, 2011;Ford and Mertz, 2013;Lee and Ha, 2013). Moreover, the structure of the bird embryo within its egg has allowed focus on specific organs, such as the chorioallantoic membrane (Branum et al, 2013;Nowak-Sliwinska et al, 2014;Yuan et al, 2014), or on large specific and circumscribed energy and nutrient pools, such as albumin and yolk (Williams, 1994;Dzialowski et al, 2009;Nangsuay et al, 2015). Finally, a compelling aspect of the bird embryo is its size, particularly when considering the embryos of the ratites (ostriches, emus, rheas), which allows surgical and other interventions not possible in many other common embryonic models (Burggren et al, 2000;Burggren et al, 2004;Dzialowski and Greyner, 2008;Khorrami et al, 2008;Shell et al, 2016), including in vitro cultivation Brand et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

2016

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-The avian embryo is a time-honored animal model for understanding vertebrate development. A key area of extensive study using bird embryos centers on developmental phenotypic plasticity of the cardio-respiratory system and how its normal development can be affected by abiotic factors such as temperature and oxygen availability. Through the investigation of the plasticity of development, we gain a better understanding of both the regulation of the developmental process and the embryo's capacity for self-repair. Additionally, experiments with abiotic and biotic stressors during development have helped delineate not just critical windows for avian cardio-respiratory development, but the general characteristics (e.g., timing and dose-dependence) of critical windows in all developing vertebrates. Avian embryos are useful in exploring fetal programming, in which early developmental experiences have implications (usually negative) later in life. The ability to experimentally manipulate the avian embryo without the interference of maternal behavior or physiology makes it particularly useful in future studies of fetal programming. The bird embryo is also a key participant in studies of transgenerational epigenetics, whether by egg provisioning or effects on the germline that are transmitted to the F1 generation (or beyond). Finally, the avian embryo is heavily exploited in toxicology, in which both toxicological testing of potential consumer products as well as the consequences of exposure to anthropogenic pollutants are routinely carried out in the avian embryo. The avian embryo thus proves useful on numerous experimental fronts as an animal model that is concurrently both of adequate complexity and sufficient simplicity for probing vertebrate cardio-respiratory development.

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“…Paraffin blocks of the CAM containing blood vessels were cut in 3-4 μm thick and placed on the poly-lysine slide, then they were incubated overnight at 45ºC. The next step of VEGF or COX-2 staining for the immunohistochemical assay was carried out as reported in previous research 18,19 . In this study, we used Hematoxylin Mayer as counterstaining.…”

Section: Immunohistochemical Assaymentioning

confidence: 99%

“…The CAMs assay is a robust and applicable technique to assess a potential drug candidate. The assay has been commonly used to study angiogenesis and tumor invasion in various type of cancer 18,19 . To verify FA's mechanism in inhibition of angiogenesis, we conducted molecular docking on COX-2 enzyme, tyrosine kinase,and VEGF-2 receptors.…”

Section: Introductionmentioning

confidence: 99%

Ferulic Acid Prevents Angiogenesis Through Cyclooxygenase-2 and Vascular Endothelial Growth Factor in the Chick Embryo Chorioallantoic Membrane Model

Ekowati¹,

Hamid²,

Diyah³

et al. 2020

tjps

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INTRODUCTION: This study designed to verify the antiangiogenic activity of ferulic acid (FA) and its potency to inhibit COX-2 and VEGF expression on CAM model. Moreover besides, we verified its mechanism of action by docking the molecule on COX-2, tyrosine kinase and VEGF-2 proteins in silico METHODS: Anti-angiogenesis assay of FA at the doses of 30, 60 and 90 μg were performed using CAM of chicken eggs with nine-day old which were stimulated by 60 ng basic fibroblast growth factor (b-FGF). Celecoxib 60 μg was used as reference drug. The inhibitory activity on VEGF and COX-2 expressions were conducted by immunohistochemistry assay (IHC). Molecular docking of FA were accomplished by Molegro Virtual Docker program ver. 5.5. on COX-2 enzyme (PDB ID 1CX2), tyrosine kinase receptor (PDB ID 1XKK) and VEGF-2 receptor (PDB ID 4ASD). RESULTS: FA at doses 30, 60, 90 μg significantly prevented angiogenesis on CAM model (p<0.05), which were represented as inhibitory activities against endothelial cell of blood vessels (42.6-70.7%) and neovascularization (43.0-86.6%). Inhibitory activities of FA against VEGF expression were stronger than its action on COX-2 expression. Molecular docking on VEGF-2 receptor result in RS value of FA was -73,844 kcal/mol, and celecoxib was -94.557 kcal/mol. RS value on tyrosine kinase of FA was -84.954 kcal/mol, while celecoxib was -93.163 kcal/mol. Docking on COX-2 receptor denoted RS value of FA was -73,416 kcal/mol, while celecoxib was -118,107 kcal/mol. DISCUSSION AND CONCLUSION: Reduction of VEGF-2 & COX-2 expression due to treatment with FA at dose range 30-90 μg seem to be related to angiogenesis inhibition, which is presented by two parameters, namely inhibition of neovascularization and endothelial cell growth in blood vessels It was concluded that FA is promising anti-angiogenic therapeutic agent especially at early stage, and this activity can be resulted from inhibitory action on COX-2 and VEGF-2 proteins.

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Application of the Chick Embryo Chorioallantoic Membrane in Neurosurgery Disease

Cited by 31 publications

References 68 publications

Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon

Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera A. Chev. (Ochnaceae) medicinal plant from Gabon

Cardio-respiratory development in bird embryos: new insights from a venerable animal model

Ferulic Acid Prevents Angiogenesis Through Cyclooxygenase-2 and Vascular Endothelial Growth Factor in the Chick Embryo Chorioallantoic Membrane Model

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