Liver function parameters, cholesterol, and phospholipid α-linoleic acid are associated with adipokine levels in overweight and obese adults

Gray, Belinda; Steyn, Frederik J.; Davies, P. L.; Vitetta, Luis

doi:10.1016/j.nutres.2014.04.001

Cited by 8 publications

(5 citation statements)

References 57 publications

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“…As one of the most important organs in the body, the liver is mainly responsible for metabolism, detoxification, protein synthesis, and production of biochemicals necessary for digestion 1 . Furthermore, the liver is susceptible to many major diseases, such as liver fibrosis, cirrhosis, hepatitis, and liver cancer, which are the main causes of morbidity and mortality 2,3 .…”

Section: Introductionmentioning

confidence: 99%

Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions

Wang

et al. 2019

Cell Death Dis

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Hepatocytes have been successfully generated from human pluripotent stem cells (hPSCs). However, the cost-effective and clinical-grade generation of hepatocytes from hPSCs still need to be improved. In this study, we reported the production of functional hepatocytes from clinical-grade human embryonic stem cells (hESCs) under good manufacturing practice (GMP) requirements. We sequentially generated primitive streak (PS), definitive endoderm (DE), hepatoblasts and hepatocyte-like cells (HLCs) from hESCs in the different stages with completely defined reagents. During hepatoblast differentiation, dimethyl sulfoxide (DMSO), transferrin, L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Vc-Mg), insulin, and sodium selenite were used instead of cytokines and FBS/KOSR. Then, hepatoblasts were differentiated into HLCs that had a typical hepatocyte morphology and possessed characteristics of mature hepatocytes, such as metabolic-related gene expression, albumin secretion, fat accumulation, glycogen storage, and inducible cytochrome P450 activity in vitro. HLCs integrated into the livers of Tet-uPA Rag2–/– Il2rg–/– (URG) mice, which partially recovered after transplantation. Furthermore, a series of biosafety-related experiments were performed to ensure future clinical applications. In conclusion, we developed a chemically defined system to generate qualified clinical-grade HLCs from hESCs under GMP conditions. HLCs have been proven to be safe and effective for treating liver failure. This efficient platform could facilitate the treatment of liver diseases using hESC-derived HLCs transplantation.

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Section: Introductionmentioning

confidence: 99%

Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions

Wang

et al. 2019

Cell Death Dis

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“…Clinical studies have demonstrated a positive correlation between ADPN and total cholesterol, HDL [ 25 , 35 , 60 , 61 ] and LDL [ 35 ] and a negative correlation with TG [ 33 , 34 , 35 , 60 ], which is confirmed by the results of our study. ADPN seems to be a strong and independent protector of cardiovascular events [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Serum Adiponectin and Leptin Concentrations in Relation to Body Fat Distribution, Hematological Indices and Lipid Profile in Humans

Lubkowska

Radecka

Bryczkowska

et al. 2015

IJERPH

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The purpose of the study was to evaluate the relationship between serum adiponectin and leptin concentrations and body composition, hematological indices and lipid profile parameters in adults. The study involved 95 volunteers (BMI from 23.3 to 53 kg/m2). Anthropometric parameters were measured: body weight and height, waist and hip circumference, waist-to-hip ratio, body fat mass (BMF), subcutaneous and visceral fat mass (SFM, VFM), lean body mass (LBM), skeletal muscle mass (SMM). In serum we determined adiponectin and leptin concentrations, extracellular hemoglobin, total bilirubin, as well as lipid metabolism (TCh, HDL-Ch, LDL-Ch, TG). Mean adipokine levels were significantly higher in women (p ≤ 0.01), adiponectin significantly negatively correlated with body height and weight, systolic blood pressure and absolute LBM and SMM values. The same relation was observed for erythroid system indicators and lipid indicators. A positive correlation was exceptionally found between adiponectin and HDL-Ch. LEP negatively correlated with some percentage rates (%LBM, %SMM). Only in women, we observed a positive correlation between LEP and body weight, BMI and WHR. Studies on ADPN and the ADPN/LEP ratio as a valuable complementary diagnostic element in the prediction and prevention of cardiovascular diseases need to be continued.

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“…However, many human adults have underlying diseases, such as obesity, hyperlipidemia, diabetes, and hypertension (Younossi et al, 2016). These diseases can directly or indirectly affect the metabolic and excretory functions of the liver, kidney, and other internal organs (Gray et al, 2014). Indeed, cumulative epidemiological and experimental studies have shown that obesity increases the risk of cancer and cancer-related mortality (Gallagher and LeRoith, 2015).…”

Section: Introductionmentioning

confidence: 99%

A 13-week subchronic toxicity study of acetaminophen using an obese rat model

et al. 2018

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Although obesity is increasing worldwide, experimental studies examining the possible association between obesity and susceptibility to chemical toxicity are limited. In the present study, we performed a 13-week toxicity study for acetaminophen (APAP), a well-known drug that exhibits hepatotoxicity as an adverse effect, using an obese rat model to investigate the differences in susceptibility between obese and normal individuals. Male F344 and obese Zucker (lean and fatty) rats were administered 0, 80, 253, 800, 2,530, or 8,000 ppm APAP in the diet for 13 weeks. No significant toxicity related to APAP treatment was observed in terms of clinical signs and hematology in all three strains. Body weight gain in F344 and lean rats was significantly decreased by 8,000 ppm APAP treatment. Significant increases in serum total cholesterol level and relative liver weights were detected in F344 rats in the highest dose group. On histopathological assessment, centrilobular hepatocellular hypertrophy was observed in the 8,000 ppm groups of F344 and lean rats, whereas no histopathological changes were induced by APAP in fatty rats. The no-observed-adverse-effect levels (NOAELs) of APAP were evaluated to be 2,530 ppm in F344 and lean rats (142.1 and 152.8 mg/kg bw/day, respectively) and more than 8,000 ppm in fatty rats (> 539.9 mg/kg bw/day). These results suggested that obese Zucker rats may be less susceptible to APAP-dependent toxicity in the liver than their lean counterparts.

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Liver function parameters, cholesterol, and phospholipid α-linoleic acid are associated with adipokine levels in overweight and obese adults

Cited by 8 publications

References 57 publications

Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions

Generation of qualified clinical-grade functional hepatocytes from human embryonic stem cells in chemically defined conditions

Serum Adiponectin and Leptin Concentrations in Relation to Body Fat Distribution, Hematological Indices and Lipid Profile in Humans

A 13-week subchronic toxicity study of acetaminophen using an obese rat model

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