“…The liver lipid classes were analyzed with thin layer chromatography (TLC-FID, Iatroscan new Mark V, Iatron Laboratories Inc., Tokyo, Japan [22]), and the tissue FA composition using gas chromatography mass spectrometry (GC-FID and GC-MS, 6890N network GC-system, Agilent Technologies Inc., Palo Alto, CA, USA). For a more detailed description of FA analyses, see Nieminen et al [14,23].…”