Liver and lung late alterations following hepatic reperfusion associated to ischemic preconditioning or <i>N</i>‐acetylcysteine

Galhardo, Maria Aparecida; Júnior, Claudemiro Quireze; Navarro, Pedro Gabriel Riboli; Morello, Ricardo José; Simões, Manuel de Jesus; Montero, Edna Frasson de Souza

doi:10.1002/micr.20359

Cited by 21 publications

(23 citation statements)

References 26 publications

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“…This applicability is supported by experimental animal studies, some done by our research team that showed local and systemic protection after ischemia-reperfusion injury [8][9][10] . NAC acts like an antioxidant beyond improving blood flow in microcirculation 11 .…”

Section: Introductionmentioning

confidence: 74%

Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

et al. 2012

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PURPOSE:Evaluate the effect of N-acetylcysteine in liver remnant after hepatectomy associated to ischemia-reperfusion injury in mice. METHODS: Male adult BALB/c mice, weighing 20-22g were used. Animals were anesthetized with ketamine (70 mg/kg) and xylazine (10 mg/kg); received N-acetylcysteine (150 mg/kg, H-IR-NAC group) or vehicle (H-IR group). Surgical procedures were performed under 10X magnification. Partial hepatectomy (30%) was followed by ischemia-reperfusion injury (30 minutes of ischemia and 60 minutes of reperfusion). Blood sample and liver tissue were removed before animal was euthanized. AST and ALT were evaluated in blood samples and histomorphological analyses were performed in remnant liver. Groups were compared by Mann-Whitney test, and it was considered significant when p<0.05. RESULTS: Biochemical evaluations showed reduced levels of ALT in NAC group (H-IR-

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Section: Introductionmentioning

confidence: 74%

Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

et al. 2012

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“…Thus, the choice of the ischemia duration is crucial for the experiment is that depending on time can lead to changes irreversible [4][5] .…”

Section: Discussionmentioning

confidence: 99%

“…During the interruption of blood supply to a tissue cell hypoxia occurs, initiating a sequence of biochemical events such as the interruption of the respiratory chain, the consumption, degradation of nucleotide triphosphate, mobilization of calcium from intracellular stores, loss of function of membrane and activation-dependent enzymes calico [2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

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The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Castro

Lauz

et al. 2012

Acta Cir. Bras.

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PURPOSE:To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS:The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed. Key words: Acetylcysteine. Lung. Reperfusion. Ischemia. Liver. Rabbits. RESUMO OBJETIVO:Estudar as lesões no fígado e no pulmão de coelhos, provocadas pela isquemia/reperfusão hepática (I/R) moduladas pelo uso da N-acetil-cisteína (NAC). MÉTODOS: Vinte e quatro coelhos distribuídos em dois grupos: Grupo controle GI (n=12) solução de glicose 5% e Grupo experimento GII (n=12) NAC. Os animais foram pré-anestesiados com maleato de acepromazina 1% e anestesiados com cloridrato de quetamina 10% e xilazina 2% via intramuscular. Os grupos GI e GII receberam solução glicosada ou NAC respectivamente via endovenosa 15min antes da oclusão do pedículo hepático (30 min). Após iniciou-se o período de reperfusão por 24h (n=6) ou 48h (n=6), terminada a reperfusão, amostras do fígado e pulmão foram coletadas para a histologia e imunoistoquímica para avaliar o comprometimento celular. RESULTADOS: Os animais do grupo GII-24h e GII-48h apresentaram arquitetura do parênquima hepático próximo do normal, quando se utilizou NAC. Os grupos GII-24h e GII-48h apresentaram menor espessura das células alveolares que os grupos GI-24h e GI-48h. A expressão da caspase 3 nas células pulmonares do grupo GII, apresentou valor menor comparada ao grupo GI. CONCLUSÃO: A N-acetil-cisteína administrada 15min prévios a lesão da isquemia/reperfusão teve um papel protetor significativo minimizando as lesões morfológicas e apoptóticas pulmonares nos períodos observados. Descritores: Acetilcisteína. Pulmão. Reperfusão. Isquemia. Fígado. Coelhos.

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“…However, despite the many benefits reported in studies using NAC in attenuating I/R damage, there are very few studies that use drug therapy for treating hemorrhagic shock 13,14 .…”

Section: N-acetylcysteine (Nac) Is a Thiol-containingmentioning

confidence: 99%

Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

Saad

Filho³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury. Key words: Acetylcysteine. Lung. Shock, Hemorrhagic. Cell Death. Rats. RESUMO OBJETIVO:Avaliar o efeito da N-acetilcisteína (NAC) combinada ao fluido de reposição volêmica na morte celular pulmonar de ratos submetidos ao choque hemorrágico (CH) controlado. MÉTODOS: Duas artérias (cálculo da PAM e exsanguinação) e uma veia (tratamentos) foram cateterizadas em 22 ratos anestesiados. Dois grupos de ratos machos albinos foram induzidos ao CH controlado com PAM de 35mmHg por 60 min. Após este período, o grupo RL foi ressuscitado com Ringer lactato e o grupo RL+NAC foi ressuscitado com Ringer lactato associado com 150mg/Kg de NAC. O grupo controle sofreu somente o procedimento cirúrgico de cateterização. Os animais sofreram eutanásia após 120 min. da ressuscitação. Amostras de tecido pulmonar foram coletadas para histopatologia, TUNEL e a imuno-expressão da caspase 3. RESULTADOS: RL apresentou maior número de células marcadas pelo TUNEL do que RL+NAC, porém sem alteração na expressão da caspase 3 em nenhum dos grupos estudados. CONCLUSÃO: A N-acetilcisteína teve um papel protetor na morte celular em modelo de choque hemorrágico controlado. Descritores: Acetilcisteína. Pulmão. Choque Hemorrágico. Morte Celular. Ratos.Saad PF et al.

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Liver and lung late alterations following hepatic reperfusion associated to ischemic preconditioning or N‐acetylcysteine

Cited by 21 publications

References 26 publications

Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

Effect of N-acetylcysteine in liver ischemia-reperfusion injury after 30% hepatectomy in mice

The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

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