The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Castro, Angela Pötter de; Castro, Miguel Angelo Martins de; Lauz, Susi; Facin, Emilio; Simões, Manuel de Jesus; Fagundes, Djalma José

doi:10.1590/s0102-86502012000100009

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…Short-term administration to rodents of such compounds as dihydrolipoyl histidinate zinc complex [50], vitamin E succinate [51], N-acetyl-cysteine [52] or (–)-epigallocatechin gallate [53] revealed their protective or antioxidant effects on liver subjected to ischemia and reperfusion. Therefore an experiment designed in a way that a single dose of a drug is administered just before the I/R procedure would be a valuable supplementation of the studies on ezetimibe-evoked pleiotropic effects.…”

Section: Discussionmentioning

confidence: 99%

Experimental research Influence of ezetimibe on selected parameters of oxidative stress in rat liver subjected to ischemia/reperfusion

Trocha¹,

Merwid-Ląd²,

Chlebda³

et al. 2014

aoms

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IntroductionIschemia/reperfusion (I/R) is considered to be one of the main causes of liver damage after transplantation. The authors evaluated the effect of ezetimibe on selected oxidative stress parameters in ischemic/reperfused (I/R) rat liver.Material and methodsRats were administered ezetimibe (5 mg/kg) (groups E and E-I/R) or saline solution (groups C and C-I/R) intragastrically for 21 days. Livers of animals in groups C-I/R and E-I/R were subjected to 60 min of partial ischemia (left lateral and median lobes) followed by 4 h of reperfusion. Alanine and asparagine aminotransferase (ALT, AST) activity was determined in blood before I/R and during reperfusion (at 15 and 240 min). After the reperfusion period, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and glutathione peroxidase (GPx) were determined in liver homogenates using colorimetric methods.ResultsEzetimibe caused a significant increase in GSH level in groups subjected to I/R (E-I/R (99.91 ±9.01) vs. C-I/R (90.51 ±8.87), p < 0.05). Additionally, under I/R the decrease of GPx activity in the drug-treated group was lower compared to the non-treated group (E-I/R (3.88 ±1.11) vs. E (5.31 ±1.83), p = 0.076). Neither ezetimibe nor I/R affected SOD or MDA levels. I/R produced a significant increase in aminotransferase levels (ALT240-0: C-I/R (42.23 ±43.56) vs. C (9.75 ±11.09), and E-I/R (39.85 ±26.53) vs. E (4.38 ±1.36), p < 0.05 in both cases; AST 240-0: E-I/R (53.87 ±17.23) vs. E (24.10 ±9.66), p < 0.05) but no effect of ezetimibe on those enzymes was found.ConclusionsEzetimibe demonstrates antioxidant properties in rat livers subjected to I/R. However, neither a hepatoprotective nor a hepatotoxic effect of ezetimibe was demonstrated, regardless of I/R.

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Section: Discussionmentioning

confidence: 99%

Experimental research Influence of ezetimibe on selected parameters of oxidative stress in rat liver subjected to ischemia/reperfusion

Trocha¹,

Merwid-Ląd²,

Chlebda³

et al. 2014

aoms

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“…1 Overfull reactive oxygen species (ROS) engendered in the liver after ischemia/reperfusion (I/R) is one of the main mechanisms contained in HI/RI. [3][4][5] Overfull productions of ROS induces remote organ injuries via oxidative stress, and inflammatory responses. 6,7 Therefore, clearance of ROS will be a potential therapeutic method for HI/RI.…”

Section: Introductionmentioning

confidence: 99%

Phycocyanin/PEG-<em>b</em>-(PG-<em>g</em>-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality

Tong

Tang

Liu

2019

IJN

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BackgroundHepatic ischemia/reperfusion-induced pancreatic islet injury (HI/RIPII) was an important pathophysiological phenomenon in clinics. In the present study, we observed the effects of phycocyanin on HI/RIPII. However, the half-life of phycocyanin was extremely short and limited its use in vivo.Materials and methodsIn order to overcome this shortcoming, poly(ethylene glycol)-b-(poly(l-glutamic acid)-g-polyethylenimine) (PEG-b-(PG-g-PEI)) was synthesized and estimated as a nanocarrier for lengthening delivery of phycocyanin through the abdominal subcutaneous injection in rats. Phycocyanin (isoelectric point=4.3) was encapsulated with PEG-b-(PG-g-PEI) via electrostatic interactions at pH 7.4.ResultsIn vitro phycocyanin was fast and efficiently encapsulated and showing efficient loading and sustained release. In vivo the anti-HI/RIPII function of phycocyanin/PEG-b-(PG-g-PEI) complex was surveyed in rats using free phycocyanin as the controls, and the results showed that phycocyanin/PEG-b-(PG-g-PEI) complex reduced HI/RIPII property and enlarged islet functionality.ConclusionThese results suggested that PEG-b-(PG-g-PEI) might be treated as a potential phycocyanin nanocarrier.

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“…In the reperfusion, while oxygen is active, xanthine oxidase metabolizes hypoxanthine in reactive oxygen species (ROS), which are: anion superoxide, hydrogen peroxide and the radical hydroxila 3,5 . Which can a cause both local and systemic injuries, especially in lipid and protein compounds, and in the cell's nucleic acids 3,6 . Allopurinol has been amply used as antioxidant method in I/R experimental models, because it presents a structure analogous to that of hypoxanthine, as it is a competitive inhibitor of xanthine oxidase, with consequent reduction in ROS production, particularly the superoxide radical [7][8][9] .…”

Section: ■ Introductionmentioning

confidence: 99%

The influence of allopurinol and post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion in Wistar rats

Akahane

Gomes²,

Paludo³

et al. 2017

Acta Cir. Bras.

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1 AbstractPurpose: To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion. Methods: Thirty rats were used. They were divided in 5 groups: (1) group A: abdominal aortic dissection only, (2) group B: ischemia and reperfusion, (3) group C: administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D: post-conditioned and (5) group E: administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores: pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle). Results: On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05. Conclusion: Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.

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The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Cited by 16 publications

References 25 publications

Experimental research Influence of ezetimibe on selected parameters of oxidative stress in rat liver subjected to ischemia/reperfusion

Experimental research Influence of ezetimibe on selected parameters of oxidative stress in rat liver subjected to ischemia/reperfusion

Phycocyanin/PEG-<em>b</em>-(PG-<em>g</em>-PEI) attenuated hepatic ischemia/reperfusion-induced pancreatic islet injury and enlarged islet functionality

The influence of allopurinol and post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion in Wistar rats

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