Live microbial cells adsorb Mg2+ more effectively than lifeless organic matter

Qiu, Xuan; Yao, Yanchen; Wang, Hongmei; Duan, Yonggang

doi:10.1007/s11707-017-0626-3

Cited by 10 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Qiu et al [32] used Haloferax volcanii DS52, a halophilic archaeon, to induce dolomite precipitation under various salinities (from 120‰ to 360‰). Qiu et al [33] demonstrated that extracellular polymers (EPS) of living cells can chelate calcium and magnesium ions, which can maintain a higher ratio of magnesium and calcium ions around the cell surface, to facilitate the formation of carbonate minerals. Deng et al [34] isolated the aerobic halophilic bacterium Halomonas marina from Qinghai Lake sediments and precipitated dolomite in low-salinity conditions.…”

Section: Introductionmentioning

confidence: 99%

The Significant Roles of Mg/Ca Ratio, Cl− and SO42− in Carbonate Mineral Precipitation by the Halophile Staphylococcus epidermis Y2

Han

Zhao

et al. 2018

Minerals

View full text Add to dashboard Cite

Carbonate precipitation induced by microorganisms has become a hot topic in the field of carbonate sedimentology, although the effects of magnesium on biomineral formation have rarely been studied. In experiments described here, magnesium sulfate and magnesium chloride were used to investigate the significant role played by Mg2+ on carbonate precipitation. In this study, Staphylococcus epidermidis Y2 was isolated and identified by 16S ribosomal DNA (rDNA) homology comparison and ammonia, pH, carbonic anhydrase, carbonate, and bicarbonate ions were monitored during laboratory experiments. The mineral phase, morphology, and elemental composition of precipitates were analyzed by XRD and SEM-EDS. Ultrathin slices of bacteria were analyzed by HRTEM-SAED and STEM. The results show that this bacterium releases ammonia and carbonic anhydrase to increase pH, and raise supersaturation via the large number of carbonate and bicarbonate ions that are released through carbon dioxide hydration catalyzed by carbonic anhydrase. The crystal cell density of monohydrocalcite is lower in a magnesium chloride medium, compared to one of magnesium sulfate. Crystals grow in the mode of a spiral staircase in a magnesium sulfate medium, but in a concentric circular pattern in a magnesium chloride medium. There was no obvious intracellular biomineralization taking place. The results presented here contribute to our understanding of the mechanisms of biomineralization, and to the role of Mg2+ in crystal form.

show abstract

Section: Introductionmentioning

confidence: 99%

The Significant Roles of Mg/Ca Ratio, Cl− and SO42− in Carbonate Mineral Precipitation by the Halophile Staphylococcus epidermis Y2

Han

Zhao

et al. 2018

Minerals

View full text Add to dashboard Cite

show abstract

“…For example, Qiu et al [55] have used Haloferax volcanii DS52, a halophilic archaeon, to induce the dolomite precipitation under various salinities (from 120 h to 360 h) and restated the important effect of high concentration of salt on the formation of microbiogenic dolomite. Qiu et al [56] have also concluded that extracellular polymers (EPS) of living cells can chelate calcium and magnesium ions, which can maintain a higher ratio of magnesium and calcium ions around the cell surface to facilitate the formation of carbonate minerals. Deng et al [57] have isolated the aerobic halophilic bacterium Halomonas marina existing in the Qinghai Lake sediments, which can precipitate dolomite in low-salinity conditions without any marine influence.…”

Section: Introductionmentioning

confidence: 99%

The Significant Role of Different Magnesium: Carbonate Minerals Induced by Moderate Halophile <em>Staphylococcus epidermis</em> Y2

Han¹,

Yu²,

Zhao³

et al. 2018

Preprint

View full text Add to dashboard Cite

Carbonate precipitation induced by microorganism has become a hot spot in the field of carbonate sedimentology, while the effect of different magnesium on biominerals has rarely been studied. Therefore, magnesium sulfate and magnesium chloride were used to investigate the significant role played on carbonate minerals. In this study, Staphylococcus epidermidis Y2 was isolated and identified by 16S rDNA homology comparison. The ammonia, pH, carbonic anhydrase, carbonate and bicarbonate ions were investigated. The mineral phase, morphology and elemental composition were analyzed by XRD and SEM-EDS. The ultrathin slices of bacteria were analyzed by HRTEM-SAED and STEM. The result showed that this bacterium could release ammonia and carbonic anhydrase to increase pH, and elevate the supersaturation via a large number of carbonate and bicarbonate ions released through carbon dioxide hydration catalyzed by carbonic anhydrase. The crystal cell density of monohydrocalcite was lower in magnesium chloride medium than that in magnesium sulfate medium. The crystal grew in a mode of spiral staircas in magnesium sulfate medium, while in a concentric circular pattern in magnesium chloride medium. There was no obvious intracellular biomineralization. This study may be helpful to further understand the biomineralization mechanism, may also provide some references for the reconstruction of paleogeological environment.

show abstract

“…EPS include functional groups that, by affecting the kinetics of the nucleation process, were shown to promote incorporation of Mg into carbonate minerals at low temperature. Several studies, indeed, suggest that the chemistry of the cell walls and the associated EPS play a crucial role 24,44,61 . Because EPS is abundantly produced when microbial communities are under ecological stress 62 , we hypothesize that more EPS were formed in the experiments carried out with high concentrations of dissolved Na + and Cl − , which in turn resulted in the mediation of carbonate minerals with a higher mol% Mg. EPS compounds bind a variety of divalent cations.…”

Section: Discussionmentioning

confidence: 99%

“…Because microbial species react differently to changes in physiochemical parameters 42 , the identification of optimal conditions for the microbial formation of Mg-rich carbonates is very challenging and caution is required before generalized conclusions can be made. There is as yet, a very limited number of studies evaluating the combined effect of several environmental factors on microbial (eco)physiology 33,43,44 .…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature, salinity and Mg2+:Ca2+ ratio on microbially-mediated formation of Mg-rich carbonates by Virgibacillus strains isolated from a sabkha environment

Disi¹,

Bontognali²,

Jaoua³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Studies have demonstrated that microbes facilitate the incorporation of Mg2+ into carbonate minerals, leading to the formation of potential dolomite precursors. Most microbes that are capable of mediating Mg-rich carbonates have been isolated from evaporitic environments in which temperature and salinity are higher than those of average marine environments. However, how such physicochemical factors affect and concur with microbial activity influencing mineral precipitation remains poorly constrained. Here, we report the results of laboratory precipitation experiments using two mineral-forming Virgibacillus strains and one non-mineral-forming strain of Bacillus licheniformis, all isolated from the Dohat Faishakh sabkha in Qatar. They were grown under different combinations of temperature (20°, 30°, 40 °C), salinity (3.5, 7.5, 10 NaCl %w/v), and Mg2+:Ca2+ ratios (1:1, 6:1 and 12:1). Our results show that the incorporation of Mg2+ into the carbonate minerals is significantly affected by all of the three tested factors. With a Mg2+:Ca2+ ratio of 1, no Mg-rich carbonates formed during the experiments. With a Mg2+:Ca2+ ratios of 6 and 12, multivariate analysis indicates that temperature has the highest impact followed by salinity and Mg2+:Ca2+ ratio. The outcome of this study suggests that warm and saline environments are particularly favourable for microbially mediated formation of Mg-rich carbonates and provides new insight for interpreting ancient dolomite formations.

show abstract

Live microbial cells adsorb Mg2+ more effectively than lifeless organic matter

Cited by 10 publications

References 34 publications

The Significant Roles of Mg/Ca Ratio, Cl− and SO42− in Carbonate Mineral Precipitation by the Halophile Staphylococcus epidermis Y2

The Significant Roles of Mg/Ca Ratio, Cl− and SO42− in Carbonate Mineral Precipitation by the Halophile Staphylococcus epidermis Y2

The Significant Role of Different Magnesium: Carbonate Minerals Induced by Moderate Halophile <em>Staphylococcus epidermis</em> Y2

Influence of temperature, salinity and Mg2+:Ca2+ ratio on microbially-mediated formation of Mg-rich carbonates by Virgibacillus strains isolated from a sabkha environment

Contact Info

Product

Resources

About