Live births from frozen human semen stored for 40 years

Széll, A.; Bierbaum, R.C; Hazelrigg, W. Brent; Chetkowski, Ryszard J.

doi:10.1007/s10815-013-9998-9

Cited by 48 publications

(24 citation statements)

References 4 publications

(6 reference statements)

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“…Young men choosing to freeze sperm samples to be used, even up to 40 years later (101), might lower their personal risk of having a child carrying a de novo RAMP mutation as well as any other de novo mutations leading to disorders that exhibit the paternal age effect. However, the societal impact of such a strategy will be less significant (at least for the present), since despite the higher risk for older fathers most children are born to younger fathers, and thus the majority of cases for these disorders are the offspring of younger men.…”

Section: Introductionmentioning

confidence: 99%

Germline Stem Cell Competition, Mutation Hot Spots, Genetic Disorders, and Older Fathers

Arnheim

Calabrese

2016

Annu. Rev. Genom. Hum. Genet.

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Some de novo human mutations arise at frequencies far exceeding the genome average mutation rate. Examples are the common mutations at one or a few sites in the genes causing achondroplasia, Noonan syndrome, multiple endocrine neoplasia 2B and Apert syndrome. These mutations are recurrent, provide a gain of function, are paternally derived and are more likely transmitted as the father ages. Recent experiments tested whether the high mutation frequencies are due to an elevated mutation rate per cell division, as expected, or an advantage of the mutant spermatogonial stem cells over wild-type stem cells. The evidence, which includes the surprising discovery of testis mutation clusters, rejects the former model but not the latter. We propose how the mutations might alter spermatogonial stem cell function and discuss how germline selection contributes to the paternal age effect, the human mutational load and adaptive evolution.

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Section: Introductionmentioning

confidence: 99%

Germline Stem Cell Competition, Mutation Hot Spots, Genetic Disorders, and Older Fathers

Arnheim

Calabrese

2016

Annu. Rev. Genom. Hum. Genet.

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“…A sample obtained prior to vasectomy and cryostored for 28 years resulted in a successful birth following intra uterine insemination [5]. Also, a sample obtained from an individual and cryostored for 40 years resulted in birth of twins following IVF-ET with ICSI [6]. But the long term effect on fertility of compromised semen quality of cancer patients, especially if they were cryopreserved during, as in this case report or soon after treatment needs to be confirmed with many more studies.…”

Section: Short Communicationmentioning

confidence: 73%

Fertility of 25 year Cryostored Sperm of a Cancer Survivor

Ivanović¹,

Jeyadevan²,

Jeyendran³

2017

Andrology

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Short CommunicationSignificant advances have been made regarding cancer treatment, and the ability to father children is becoming increasingly important to long term survivors. Since infertility is often the result of cancer treatment, semen cryopreservation prior to treatment is very practical, simple, relatively inexpensive, and highly recommended. Cryopreservation of semen is a well-established procedure since the birth of a baby from frozen thawed semen was first reported in early 1950's [1]. The cryopreservation procedures do affect the semen quality, especially if the semen quality is compromised, as is often observed with cancer patients. However, with the advent of intracytoplasmic sperm injection (ICSI) and within vitro fertilization embryo transfer (IVF-ET) procedure, cryostorage of compromised semen is feasible and should be considered. To our knowledge there is only one other report on fertility of 21 year cryostored semen of a cancer survivor [2]. At present many young cancer patients are requesting semen cryopreservation, and additional follow-up studies utilizing the cryostored semen are not only needed, but should be extended to cover many more years of cryostorage. This report describes a confirmed fertility of 25 year cryostored semen of a cancer survivor. A 17 year old was diagnosed with chronic granulocytic leukemia in March of 1985 and was treated with busulphan and thioguanine, which culminated in allogenic bone marrow transplant with cyclosporine. During treatment 5 different ejaculates obtained in June were cryopreserved in ampoules (Table 1). The patient remained cancer free with no evidence of recurrence, except for persistent azoospermia as confirmed following routine semen analyses performed in 1992, 2004 and 2010. Based on this finding an IVF-ET with ICSI procedure was initiated using the samples cryopreserved in 1985. Of the 13 mature eggs retrieved, 11 fertilized, yielding 5 blastocysts, 3 of which were cryopreserved for future use and 2 were transferred with a successful outcome, resulting in dizygotic twin boys. Paternity testing confirmed the identity of the boys. The germinal epithelium will be depleted by exposure to more than 600cGy radiation, and chemotherapeutic agents can often deplete germinal epithelium as well. Although it has been demonstrated in rodent mutation rates are high during and soon after therapy but in human it appears that the chromosomal aneuploidy is more frequent [3]. However, whether the observed DNA damage is reflected into a parallel increase in fetal malformation is not known. Only a relatively low number of men who store semen prior to cancer treatment utilize their semen within a few years. The reason may that the patients have restored their fertility, did not plan to have children or succumbed to death [4]. Fertility of long term cryostored semen has been previously documented. A sample obtained prior to vasectomy and cryostored for 28 years resulted in a successful birth following intra uterine insemination [5]. Also, a sample obtained from an i...

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“…Equally, the perceived high cost of cryopreservation and storage might have a role to play, even though robust cost-benefit analyses have shown sperm cryopreservation to be more cost-effective than post-therapeutic fertility management [19]. It has been evidenced that long-time storage does not seem to affect the fertilisation potential of sperm, as recently reported after 40 years of storage [24].…”

Section: Particular Considerations Regarding the Cryopreservation Of mentioning

confidence: 98%

Clinical Outcomes of Assisted Reproductive Techniques Using Cryopreserved Gametes and Embryos in Human Medicine

Waterstone¹,

Anastácio²,

Rodriguez‐Wallberg³

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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The methods of cryopreservation play a key role in assisted reproductive technique (ART) treatments, as they increase the efficacy of the treatments by allowing banking of supernumerary embryos for later use. It has been recently proposed that these methods could also increase the safety of ART treatments, by reducing complications such as ovarian hyperstimulation during early pregnancy; thus, the policy of total freeze for later differed transfer of embryos has been proposed. Also of great importance, cryopreservation of oocytes and spermatozoa has permitted gamete storage for long term facilitating practical routines such as the gamete banking for third-party reproductive treatment. In this chapter, the clinical indications and treatment outcomes will be revised and data updated on the safety of using cryopreservation methods in ART treatments.

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Live births from frozen human semen stored for 40 years

Abstract: Capsule Healthy twin girls were born from IVF using frozen semen stored for approximately 40 years.

Cited by 48 publications

References 4 publications

Germline Stem Cell Competition, Mutation Hot Spots, Genetic Disorders, and Older Fathers

Germline Stem Cell Competition, Mutation Hot Spots, Genetic Disorders, and Older Fathers

Fertility of 25 year Cryostored Sperm of a Cancer Survivor

Clinical Outcomes of Assisted Reproductive Techniques Using Cryopreserved Gametes and Embryos in Human Medicine

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