Live birth after vitrification of in vitro matured human oocytes

Chian, Ri‐Cheng; Gilbert, Lucy; Huang, Jacky; Demirtaş, Ezgi; Holzer, Hananel; Benjamin, Alice; Buckett, William; Tulandi, Togas; Tan, Seang Lin

doi:10.1016/j.fertnstert.2007.11.088

Cited by 150 publications

(101 citation statements)

References 27 publications

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“…Significant progress has been made to improve pregnancy and implantation rates with in-vitro matured oocytes [18], but the mechanisms regulating early folliculogenesis and oocyte maturation in the human are still poorly understood [19]. Further research remains necessary to address the mechanism of oocyte maturation in order to refine culture conditions and improve the implantation rate of in vitro matured oocytes.…”

Section: Discussionmentioning

confidence: 99%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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Purpose This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification. Methods A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2). Results Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p<0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p<0.05). Conclusion IVM procedure is more efficient when it is performed before oocyte vitrification.

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Section: Discussionmentioning

confidence: 99%

In-vitro maturation of human oocytes: before or after vitrification?

Fasano

Demeestere

Englert

2012

J Assist Reprod Genet

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“…Until now, vitrification has been widely used for the cryopreservation of human oocytes [18,24,25], in vitro matured oocytes [26,27] pronuclear stage , cleavage stage [5,7,14,18,[29][30][31][32][33][34], or blastocyst-stage [8,18,[35][36][37][38][39][40][41][42]. However there are few publications that show clinical data on the basis of vitrification versus slow freezing, especially for the cleavage stage [5,8].…”

Section: Introductionmentioning

confidence: 99%

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Valojerdi

Eftekhari‐Yazdi

Karimian

et al. 2009

J Assist Reprod Genet

145

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Purpose The objective of this retrospective study was to evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate, post-warming embryo morphology and clinical outcomes. Methods The embryos of 305 patients at cleavage stages were cryopreserved either with vitrification (153 patients) or slow-freezing (152 patients) methods. After warming; the survival rate, post-warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups. Result(s) In the vitrification group versus slow freezing group, the survival rate (96.9% vs. 82.8%) and the postwarmed excellent morphology with all blastomeres intact (91.8% vs. 56.2%) were higher with an odds ratio of 6.607 (95% confidence interval; 4.184-10.434) and 8.769 (95% confidence interval; 6.460-11.904), respectively. In this group, the clinical pregnancy rate (40.5% vs. 21.4%) and the implantation rate (16.6% vs. 6.8%) were also higher with an odds ratio of 2.427 (95%confidence interval; 1.461-4.033) and 2.726 (95% confidence interval; 1.837-4.046), respectively. Conclusion(s) Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos. Vitrification provides a higher survival rate, minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.

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“…Because cumulus cells are critical for successful in vitro maturation, we chose to cryopreserve oocytes after in vitro maturation, at a stage when interaction between oocyte and somatic cells is no longer needed. The choice to cryopreserve at the mature stage was also based on the fact that a few pregnancies have been achieved from oocytes matured in vitro and vitrified at the metaphase II stage [12]. Cao and Chian [13] have also reported that cryopreservation at the mature stage after IVM is a more valuable option.…”

Section: Discussionmentioning

confidence: 99%