Live birth after transfer of a tripronuclear embryo: An intracytoplasmic sperm injection as a combination of microarray and time-lapse technology

Yalçınkaya, Ender; Özay, A.; Ergin, E.; Oztel, Z.; Ozornek, H.

doi:10.4274/tjod.45144

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…Consistent with this, a live birth after the transfer of a euploid tripronuclear embryo in a 36 years aged woman with diminished ovarian reserve has been reported. 16 The capability of self-correction of the 3PN embryos or the microsurgical removal of the retained pronucleus may provide a chance to rescue 3PN embryos. 7,13 The limitation of the latter procedure involves the difficulty of choosing the right pronucleus to remove which could lead to inheriting two sets of maternal or paternal chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst Formation and Aneuploidy Rate of Tripronuclear Embryos

GÜLŞEN,

GÜRGAN

2023

TJRMS

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Human embryos generated from abnormal fertilization are usually discarded in assisted reproductive technologies (ART) practice. This study aims to investigate the potential of developing into blastocysts and the chromosomal constitution of tripronuclear (3PN) zygotes. Material and Methods: This study consists of patients who had at least one 3PN embryo after a conventional ART cycle and a subsequent preimplantation genetic testing for aneuploidy (PGT-A) between 2020 and 2022 in our IVF unit. Demographic data, cycle outcomes, blastulation and euploidy rates of 3PN zygotes were retrospectively analyzed. Results: Among 108 patients with a history of PGT-A for diverse indications, 17 had a total of 29 embryos with abnormal pronuclei (3PN). Of those, 9 embryos achieved to develop into a blastocyst and, 6 of them were furtherly evaluated with a fluorescence in situ hybridization (FISH) analysis. The ratio of 3PN embryos to all fertilized embryos (3PN/2PN+3PN) was %21. Although 31% of 3PN embryos reached to the blastocyst stage, all of the biopsied embryos were reported to be aneuploid and clinically discarded later. Conclusion: Based on the low/null euploidy rate; it is mandatory to perform PGT-A before the transfer of a 3PN-derived embryo, particularly in poor prognosis patients with a limited number of embryos. Instead of genetic analysis, routine discard of 3PN-derived blastocysts would be more cost-effective in women with normal ovarian response. Further randomized prospective studies are required to determine the subgroup of patients who could benefit more from genetic testing of 3PN zygotes.

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Section: Discussionmentioning

confidence: 99%

Blastocyst Formation and Aneuploidy Rate of Tripronuclear Embryos

GÜLŞEN,

GÜRGAN

2023

TJRMS

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“…3PN embryos usually form by fertilisation with two spermatozoa, or polar body extrusion failure 54 . They are associated with spontaneous abortions, however a euploid live baby has been born from a tripronuclear embryo 55 . Patients providing material unsuitable for use in their treatment received no compensation.…”

Section: Methodsmentioning

confidence: 99%

The first mitotic division of human embryos is highly error prone

et al. 2022

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Human beings are made of ~50 trillion cells which arise from serial mitotic divisions of a single cell - the fertilised egg. Remarkably, the early human embryo is often chromosomally abnormal, and many are mosaic, with the karyotype differing from one cell to another. Mosaicism presumably arises from chromosome segregation errors during the early mitotic divisions, although these events have never been visualised in living human embryos. Here, we establish live cell imaging of chromosome segregation using normally fertilised embryos from an egg-share-to-research programme, as well as embryos deselected during fertility treatment. We reveal that the first mitotic division has an extended prometaphase/metaphase and exhibits phenotypes that can cause nondisjunction. These included multipolar chromosome segregations and lagging chromosomes that lead to formation of micronuclei. Analysis of nuclear number and size provides evidence of equivalent phenotypes in 2-cell human embryos that gave rise to live births. Together this shows that errors in the first mitotic division can be tolerated in human embryos and uncovers cell biological events that contribute to preimplantation mosaicism.

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“…Various studies have shown that these tripronuclear human oocytes do not always develop into triploid embryos. 11 , 12 Although births derived from triploid zygotes have been reported, such infants generally do not live long after birth and only few have been reported as surviving for an unusual duration. 13 Thus, it is important to analyze the chromosomal abnormalities of embryos to determine whether they are suitable for embryo transfer.…”

Section: Introductionmentioning

confidence: 99%

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

Takahashi

Hirata

Otsuki

et al. 2022

Reprod Medicine & Biology

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Purpose This study aimed to analyze whether tripronuclear (3PN) zygotes, with two normal‐sized PNs and an additional smaller PN (2.1PN), can be used for embryo transfer. Method(s) A retrospective embryo cohort study was conducted on 695 patients who underwent intracytoplasmic sperm injection treatment. Blastocyst formation rates were compared between 2.1PN and 2PN zygotes and PGT‐A analysis was performed on 15 blastocysts derived from 2.1PN zygotes. Result(s) Blastocyst formation rates were comparable between 2.1PN (43.8%) and 2PN zygotes (54.8%; p = 0.212). The rates of blastocysts with good morphology derived from 2.1 PN and 2PN zygotes were 18.8% and 25.5%, respectively. No significant differences were detected ( p = 0.383). All of the analyzed blastocysts were diploid; however, 13 of these were found to be aneuploid, with a further two being mosaic. Conclusion Our results suggest that 2.1PN embryos can reach blastocyst stage. These blastocysts were diploid, however, predominantly aneuploid, and therefore could not be used for embryo transfer.

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Live birth after transfer of a tripronuclear embryo: An intracytoplasmic sperm injection as a combination of microarray and time-lapse technology

Cited by 8 publications

References 9 publications

Blastocyst Formation and Aneuploidy Rate of Tripronuclear Embryos

Blastocyst Formation and Aneuploidy Rate of Tripronuclear Embryos

The first mitotic division of human embryos is highly error prone

Are tri‐pronuclear embryos that show two normal‐sized pronuclei and additional smaller pronuclei useful for embryo transfer?

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