Live birth after ovarian tissue transplant

Lee, D. M.; Yeoman, Richard R.; Battaglia, David E.; Stouffer, Richard L.; Zelinski, Mary B.; Fanton, John W.; Wolf, Don P.

doi:10.1038/428137a

Cited by 168 publications

(93 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first live birth in a primate after heterotopic ovarian transplantation has been reported [120]. In human, ovarian function has been restored in two patients for at least 2 years after transplanting ovarian tissue to the forearm.…”

Section: Heterotopic Autograftingmentioning

confidence: 99%

Human oocyte and ovarian tissue cryopreservation and its application

Tao

Valle

2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To review the recent progress in human oocyte and ovarian tissue cryopreservation, and in the application of these two technologies for preserving female fertility of patients who are undergoing cancer treatment. Design The literature on human oocyte and ovarian tissue freezing was searched with PubMed. The scientific background, current developments and potential future applications of these two methods were reviewed.

show abstract

Section: Heterotopic Autograftingmentioning

confidence: 99%

Human oocyte and ovarian tissue cryopreservation and its application

Tao

Valle

2008

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Follicle development is relatively easy to monitor, and the tissue is easily accessible for ovum pick up [14]. Successful fertilization and pregnancy with oocytes from subcutaneously transplanted fresh ovarian tissue have been reported in a primate [15]. In humans, a 4-cell embryo was produced from subcutaneously transplanted cryopreserved ovarian tissue, but it did not result in conception [16].…”

Section: Introductionmentioning

confidence: 99%

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Lee

et al. 2008

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To analyze the gap junction proteins connexin 37 (Cx37) and connexin 43 (Cx43) after subcutaneous transplantation of cryopreserved mouse ovarian tissue. Methods Expression of gap junction genes was assessed by immunohistochemistry and real-time polymerase chain reaction (PCR) in transplanted cryopreserved ovarian tissue compared with that of normal ovarian tissue. Apoptosis of ovarian cells was evaluated by using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphates nick end-labeling method.Results After subcutaneous transplantation, Cx37 and Cx43 mRNA and protein expression were significantly lower in cryopreserved than in normal ovarian tissue. Apoptosis was increased in granulosa cells from antral follicles of the cryopreserved tissue. Conclusion After cryopreservation and subcutaneous transplantation of ovarian tissue, proteins forming gap junctions between oocytes and granulosa cells are under-expressed compared with normal controls.

show abstract

“…The only report of a live young derived from a subcutaneously located graft occurred in a monkey when the resulting embryos were transferred to a gestational surrogate (Lee et al 2004). Oocytes collected from human ovarian tissue grafted under the skin of the forearm have proved difficult to successfully fertilize and establish a pregnancy (Oktay et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Yang¹,

Cox²,

Jenkin³

et al. 2006

Reproduction

View full text Add to dashboard Cite

Ovarian tissue cryopreservation and subsequent transplantation can restore fertility in cancer patients. This study used a mouse ovarian grafting model to investigate whether the graft site (bursal cavity, the kidney capsule or subcutaneous) influences the number, fertilization rate and developmental potential of oocytes recovered from grafts and whether using a standard gonadotrophin stimulation protocol would increase oocyte yield from the grafts. Mouse ovarian tissue was grafted into four week old mice and collected three weeks later. Graft recipients were treated either with or without exogenous gonadotrophin stimulation prior to graft collection. Grafted ovaries yielded oocytes that were either at the germinal vesicle (GV) stage or mature metaphase II (MII) stage at collection. These GV oocytes were matured before in vitro fertilization (IVF), while the MII oocytes underwent IVF immediately. Oocytes collected from the oviducts of non-grafted superovulated mice of the same age served as controls. Two-cell embryos were transferred to pseudopregnant recipients and recovered at day 15 of gestation or left to go to term. Graft retrieval and the number of oocytes from each graft were lowest from the subcutaneous graft site. The number of two-cell embryos produced was significantly higher for oocytes from the grafts to the bursa as compared with the other sites. All graft sites gave rise to embryos with comparable implantation rates and developmental potential to fetuses and offspring following transfer. However, the oocytes from grafted ovaries had a significantly lower developmental potential when compared with the control group. Stimulation with exogenous gonadotrophins did not significantly increase oocyte yield from grafted ovaries but did enhance oocyte maturation and development. In conclusion, graft site affects the number and quality of oocytes produced from ovarian grafts.

show abstract

Live birth after ovarian tissue transplant

Cited by 168 publications

References 8 publications

Human oocyte and ovarian tissue cryopreservation and its application

Human oocyte and ovarian tissue cryopreservation and its application

Abnormally low expression of connexin 37 and connexin 43 in subcutaneously transplanted cryopreserved mouse ovarian tissue

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Contact Info

Product

Resources

About