Human oocyte and ovarian tissue cryopreservation and its application

Tao, Tao; Valle, Alfonso Del

doi:10.1007/s10815-008-9236-z

Cited by 77 publications

(51 citation statements)

References 119 publications

(144 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, further research is needed to enhance the freezing and revascularization processes. To date, only 15 live births have been reported, none of which were derived from cryopreserved prepubertal tissue [15,16,17]. Of note, most BPES type I patients are diagnosed in infancy.…”

Section: Foxl2 Impairment In Bpesmentioning

confidence: 99%

<i>FOXL2 </i>Impairment in Human Disease

Verdin¹,

Baere²

2012

Horm Res Paediatr

View full text Add to dashboard Cite

FOXL2 encodes a forkhead transcription factor that plays important roles in the ovary during development and in post-natal, adult life. Here, we focus on the clinical consequences of FOXL2 impairment in human disease. In line with other forkhead transcription factors, its constitutional genetic defects and a somatic mutation lead to developmental disease and cancer, respectively. More than 100 unique constitutional mutations and regulatory defects have been found in blepharophimosis syndrome (BPES), a complex eyelid malformation associated (type I) or not (type II) with premature ovarian failure (POF). In agreement with the BPES phenotype, FOXL2 is expressed in the developing eyelids and in fetal and adult ovaries. Two knock-out mice and at least one natural animal model, the Polled Intersex Syndrome goat, are known. They recapitulate the BPES phenotype and have provided many insights into the ovarian pathology. Only a few constitutional mutations have been described in nonsyndromic POF. Moreover, a recurrent somatic mutation p.C134W was found to be specific for adult ovarian granulo-sa cell tumors. Functional studies investigating the consequences of FOXL2 mutations or regulatory defects have shed light on the molecular pathogenesis of the aforementioned conditions, and contributed considerably to genotype-phenotype correlations. Recently, a conditional knock-out of Foxl2 in the mouse induced somatic transdifferentiation of ovary into testis in adult mice, suggesting that Foxl2 has an anti-testis function in the adult ovary. This changed our view on the ovary and testis as terminally differentiated organs in adult mammals. Finally, this might have potential implications for the understanding and treatment of frequent conditions such as POF and polycystic ovary syndrome.

show abstract

Section: Foxl2 Impairment In Bpesmentioning

confidence: 99%

<i>FOXL2 </i>Impairment in Human Disease

Verdin¹,

Baere²

2012

Horm Res Paediatr

View full text Add to dashboard Cite

show abstract

“…Oocyte cryopreservation offers a range of perspectives as it: (i) permits women to cryopreserve oocytes prior to gonadotoxic radio-or chemotherapy and/or ovariectomy (Tao and Del Valle, 2008;Ata et al, 2010); (ii) allows women to delay childbearing (Stoop et al, 2011(Stoop et al, , 2014; (iii) eliminates donor-recipient endometrium synchronization problems and (iv) avoids ethical and legal concerns regarding supernumerary cryopreserved embryos and embryo ownership (Schoolcraft et al, 2009). Slow freezing has been the traditional cryopreservation method until the introduction of the vitrification protocol by Kuwayama et al (2005).…”

Section: Introductionmentioning

confidence: 99%

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Munck

Petrussa

Verheyen³

et al. 2015

MHR: Basic Science of Reproductive Medicine

View full text Add to dashboard Cite

Oocyte vitrification has been introduced into clinical settings without extensive pre-clinical safety testing. In this study, we analysed major safetyaspects of human oocyte vitrification in a high security closed system: (i) chromosomal meiotic segregation, (ii) embryonic developmental kinetics and (iii) DNA (hydroxy)methylation status. Fresh and vitrified sibling oocytes from young donors after intracytoplasmic sperm injection (ICSI) were compared in three different assays. Firstly, the chromosomal constitution of the fertilized zygotes was deduced from array comparative genomic hybridization results obtained from both polar bodies biopsied at Day 1. Secondly, embryo development up to Day 3 was analysed by time-lapse imaging. Ten specific time points, six morphokinetic time intervals and the average cell number on Day 3 were recorded. Thirdly, global DNA methylation and hydroxymethylation patterns were analysed by immunostaining on Day 3 embryos. The nuclear fluorescence intensity was measured by Volocity imaging software. Comprehensive chromosomal screening of the polar bodies demonstrated that at least half of the zygotes obtained after ICSI of fresh and vitrified oocytes were euploid. Time-lapse analysis showed that there was no significant difference in cleavage timings, the predictive morphokinetic time intervals nor the average cell number between embryos developed from fresh and vitrified oocytes. Finally, global DNA (hydroxy)methylation patterns were not significantly different between Day 3 embryos obtained from fresh and from vitrified oocytes. Our data further consolidate the safety of the oocyte vitrification technique. Nevertheless, additional testing in young and older sub-fertile/infertile patients and sound follow-up studies of children born after oocyte cryopreservation remain mandatory.

show abstract

“…In spite of the hitherto supported experimental nature of ovarian tissue cryopreservation and transplantation (OTT/OTC) as methods implying unsafety, ineffi ciency and uncertainty [1][2][3][4][5], most recently they tend to be categorized as well-established [6][7] rather than experimental procedures, although they are invariably optimized [7].  represents a solution for women that suffer premature ovarian menopause (POM) and infertility, especially in the fi eld of oncofertility (preservation of fertility threatened by oncologic diseases or gonadotoxic treatment) [8], thus restoring their "quality of life".…”

Section: Introductionmentioning

confidence: 99%

Ovarian Tissue Cryopreservation / Transplantation for Social Reasons: Between (“Good”) Medicalization and Medical Treatment.

Voultsos¹

2017

Glob J Fertil Res

View full text Add to dashboard Cite

Background: Ovarian tissue cryopreservation / transplantation (OTC/OTT) has recently been considered as a well-established rather than an experimental method (at least adopting pragmatic optimism) and turned into a realistic option for a healthy woman needing to delay her childbearing and defer her menopause. Thus, going beyond "normality" through medicalizing a normal aspect of a woman's life or addressing a medical problem? Discussion: Ovarian tissue has a dual nature: it is an organ with a dual function: endocrine and gametogenesis. Premature ovarian failure may be an unhealthy state insofar as health is captured in a holistic way according to Nordenfelt's or especially Richman's theory, especially if Elson's theory of hormonal hierarchy is true. Childbearing age is broadly captured, involving what for the time being is meant as early post-menopausal stage, since human life expectancy has expanded signifi cantly but the childbearing lifespan of a woman has not. On the other hand, it is not clear whether the social environment represents a social determinant of public health or a mere cause of a woman's ill health at least in so far as autonomy is captured through the relational model of autonomy. Conclusion:OTC/OTT for a "healthy" woman are carried out in a blurred and clearly shifting gray zone between normality and pathology when performed at a childbearing age, thus fostering an already existing situation. OTT/OTC constitute "good" (ethically acceptable) medicalization which is subtly diversifi ed from medical treatment as being divided from it with a thin blurring borderline, thus being on the cusp of becoming medical treatment based on solid medical reasons.

show abstract

Human oocyte and ovarian tissue cryopreservation and its application

Cited by 77 publications

References 119 publications

<i>FOXL2 </i>Impairment in Human Disease

<i>FOXL2 </i>Impairment in Human Disease

Chromosomal meiotic segregation, embryonic developmental kinetics and DNA (hydroxy)methylation analysis consolidate the safety of human oocyte vitrification

Ovarian Tissue Cryopreservation / Transplantation for Social Reasons: Between (“Good”) Medicalization and Medical Treatment.

Contact Info

Product

Resources

About