Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment

Abstract

Litorisediminicola beolgyonensis gen. nov., sp. nov., isolated from a coastal sediment Strain BB-MW24 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the predominant fatty acid. The polar lipid profile in strain BB-MW24 T was similar to those of members of the genera Ponticoccus and Roseivivax, but one unidentified phospholipid was found as a major polar lipid only in strain BB-MW24 T . The DNA G+C content was 68.9 mol%. The phylogenetic data and differential chemotaxonomic and phenotypic properties revealed that strain BB-MW24 T represents a novel species in a new genus within the class Alphaproteobacteria, for which the name Litorisediminicola beolgyonensis gen. nov., sp. nov. is proposed; the type strain of Litorisediminicola beolgyonensis is BB-MW24 T (5KCTC 32139 T 5CCUG 62953 T ).A variety of tidal flats of western and southern coasts of the Korean peninsula have been proven to be good environments from which to isolate novel bacterial taxa (e.g. Kwon et al., 2006;Yoon et al., 2009Yoon et al., , 2010 Baik et al., 2010;Lee et al., 2012). During the isolation of micro-organisms from a tidal flat of the southern coast of the peninsula, where cockles (Tegillarca granosa) are known to inhabit and high contents of germanium and selenium are found, many bacterial strains have been isolated and characterized taxonomically. One of these isolates, designated BB-MW24 T , is described in this study. Comparative 16S rRNA gene sequence analysis showed that this strain was most phylogenetically closely related to the genera Roseivivax and Ponticoccus of the class Alphaproteobacteria. The genus Roseivivax was created by Suzuki et al. (1999) T by using a polyphasic characterization that included determination of the phenotypic and chemotaxonomic properties and a detailed phylogenetic investigation based on 16S rRNA gene sequences. Strain BB-MW24T was isolated by the dilution-plating technique on marine agar 2216 (MA; BD) at 25 u C and cultivated routinely on MA at 30 u C. Strain BB-MW24 T was maintained on MA at 4 u C for short-term preservation and as a glycerol suspension (20 %, w/v, in distilled water) at 280 u C for long-term preservation. P. litoralis DSM 18986 T and R. lentus S5-5 T were used as reference strains for phenotypic characterization, fatty acid analysis and sequencing of the DNA gyrase B subunit gene (gyrB). Cell morphology was examined by using light microscopy (BX51; Olympus) and transmission electron microscopy (CM-20; Philips). The latter technique was also used to assess the presence of flagella on cells from an exponentiallyThe GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain BB-MW24T and the gyrB sequences of strain BB-MW24 T , Ponticoccus litoralis DSM 18986 T and Roseivivax lentus S5-5 T are JQ807220, JX444963, JX444964 and JX444965, respectively.A supplementary figure is available with the online version of this paper.

Pseudohalocynthiibacter aestuariivivens gen. nov., sp. nov., isolated from a tidal flat

“…The Roseobacter clade, belonging to the order Rhodobacterales of the class Alphaproteobacteria (Garrity et al , 2005, 2006), is known to be one of the most abundant groups in marine environments (Rappé et al , 2000; Buchan et al , 2005). From our studies screening novel bacteria from a variety of tidal flats on the Yellow Sea and the South Sea of the Korean Peninsula, many novel bacterial taxa belonging to the Roseobacter clade have been described (Yoon et al , 2009a, b, 2010, 2013a, b; Jung et al , 2010; Park et al , 2014a, b). In this study, we describe a bacterial strain, designated BS-W9 T , which was isolated from a tidal flat of Boseong on the South Sea of the Korean Peninsula.…”

Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment

“…A variety of tidal flats on the western and southern coasts of the Korean peninsula have proved to be good environments from which to isolate novel bacterial taxa belonging to the Roseobacter clade (Yoon et al, 2009a(Yoon et al, , b, 2010(Yoon et al, , 2013aJung et al, 2010;Kim et al, 2010;Park et al, 2011). During screenings of novel bacteria from a tidal flat at Boseong on the southern coast of South Korea, many novel bacterial taxa have been isolated and characterized taxonomically Yoon et al, 2013b).…”

Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat

“…From our studies aimed at discovering novel bacteria from marine environments of the South Sea of the Korean peninsula, many new bacterial taxa belonging to the class Proteobacteria have been described (Lee et al , 2013; Park et al , 2013; Park & Yoon, 2013; Yoon et al , 2013a, b). The majority of these novel taxa belong to the family Rhodobacteraceae within the class Alphaproteobacteria (Garrity et al , 2005, 2006), which is known to be one of the most abundant groups in marine environments (Rappé et al 2000; Buchan et al 2005).…”