Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, aerobic, non-motile and pleomorphic (coccoid, ovoid or rod-shaped) bacterial strain, BS-W15 T , isolated from a tidal flat sediment at Boseong in South Korea, was characterized taxonomically. Strain BS-W15 T grew optimally at 25 6C, at pH 7.0-8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees, based on 16S rRNA gene sequences, revealed that strain BS-W15 T joined the cluster comprising the type strains of Profundibacterium mesophilum, Hwanghaeicola aestuarii, M. pelagius and M. salinus, showing 93.5-96.4 % sequence similarities. Strain BS-W15 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the predominant fatty acid. The polar lipid profile of strain BS-W15 T contained phosphatidylcholine and phosphatidylglycerol as major components, differentiating it from those of the type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. The DNA G+C content of strain BS-W15 T was 58.7 mol%. The differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrate that strain BS-W15 T is distinct from type strains of P. mesophilum, H. aestuarii, M. pelagius and M. salinus. On the basis of the data presented, strain BS-W15 T is considered to represent a novel genus and species, for which the name Boseongicola aestuarii gen. nov., sp. nov. is proposed. The type strain is BS-W15 T (5KCTC 32576 T 5CECT 8489 T ).

Aliiroseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three species of the genus Roseovarius as Aliiroseovarius crassostreae comb. nov., Aliiroseovarius halocynthiae comb. nov. and Aliiroseovarius sediminilitoris comb. nov.

“…Strain GYSW-22 T and the type strains of R. halocynthiae , R. crassostreae and R. sediminilitoris formed a cluster in the NJ, ML and MP trees, and this cluster formed a phylogenetic lineage independent of those of the type strains of other species of the genus Roseovarius (Figs 1, S2 and S3). The clustering of the type strains of R. halocynthiae , R. crassostreae and R. sediminilitoris which is distinct from the type strains of other species of the genus Roseovarius has been shown in other studies (Choi et al , 2013; Hameed et al , 2014; Park et al , 2014c, d; Rajasabapathy et al , 2014). Taking these data into account, it is reasonable to place strain GYSW-22 T and the type strains of R. crassostreae , R. halocynthiae and R. sediminilitoris as members of a new genus, not the genus Roseovarius .…”

“…Comparative 16S rRNA gene sequence analysis showed that this novel strain is phylogenetically most closely related to some species of the genus Roseovarius of the class Alphaproteobacteria . It is known that members of the genus Roseovarius do not form a monophyletic cluster encompassed by the corresponding genus and they may have to be taxonomically re-evaluated (Hameed et al , 2014; Kim et al , 2014; Park et al , 2014a, c, d; Rajasabapathy et al , 2014). Roseovarius marinus has been recently reclassified as a member of the genus Pacificibacter (Park et al , 2014a).…”

The Biogeographical Distribution of Benthic Roseobacter Group Members along a Pacific Transect Is Structured by Nutrient Availability within the Sediments and Primary Production in Different Oceanic Provinces

“…For instance, Sedimentitalea , which is found in both, the DNA- and RNA-based communities, was originally isolated from sandy sediments of the South China Sea (Sun et al, 2010 ; Breider et al, 2014 ). The type strain of Boseongicola derived from tidal-flat sediments (Park et al, 2014 ) and Loktanella from a deep subseafloor sediment off the coast of Japan (Van Trappen et al, 2004 ; Tsubouchi et al, 2013 ). While Loktanella and Sulfitobacter -affiliated OTUs were found in coastal North Sea sediments (Kanukollu et al, 2016 ), none of the above mentioned genera were previously detected in deep-sea sediments of open ocean sites.…”

Pseudohalocynthiibacter aestuariivivens gen. nov., sp. nov., isolated from a tidal flat

“…The Roseobacter clade, belonging to the order Rhodobacterales of the class Alphaproteobacteria (Garrity et al , 2005, 2006), is known to be one of the most abundant groups in marine environments (Rappé et al , 2000; Buchan et al , 2005). From our studies screening novel bacteria from a variety of tidal flats on the Yellow Sea and the South Sea of the Korean Peninsula, many novel bacterial taxa belonging to the Roseobacter clade have been described (Yoon et al , 2009a, b, 2010, 2013a, b; Jung et al , 2010; Park et al , 2014a, b). In this study, we describe a bacterial strain, designated BS-W9 T , which was isolated from a tidal flat of Boseong on the South Sea of the Korean Peninsula.…”