Lithium Chloride Modulates Adipogenesis and Osteogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells

Tang, Linjun; Chen, Yu; Pei, Fuxing; Zhang, Hui

doi:10.1159/000430340

Cited by 62 publications

(41 citation statements)

References 33 publications

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“…The important role of lithium in bone formation was recently suggested, based on results from in vitro and in vivo studies. An in vitro study illustrated that LiCl motivated Wnt signalling by inhibiting the activity of GSK3β and by counteracting inhibition of the Wnt pathway mediated by DKK1 (an inhibitor of Wnt) . LiCl may continue to enhance bone formation and reduce bone resorption, even in osteoporotic conditions, as shown in rats .…”

Section: Discussionsupporting

confidence: 89%

Lithium enhances alveolar bone formation during orthodontic retention in rats

Pan

Yin

et al. 2017

Orthod Craniofacial Res

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Section: Discussionsupporting

confidence: 89%

Lithium enhances alveolar bone formation during orthodontic retention in rats

Pan

Yin

et al. 2017

Orthod Craniofacial Res

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“…It has been demonstrated that some specific proteins [31][32][33], miRNAs [34,35] and molecules [36][37][38] are involved in the regulation of osteogenic differentiation in BMSCs. These findings suggested that osteogenic differentiation is a complicated multifactorial process.…”

Section: Discussionmentioning

confidence: 99%

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Yuan

Yan

Gong

et al. 2017

Cell Physiol Biochem

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Background/Aims: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrowderived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. Methods: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated. Cell proliferation was measured by performing cell counting and EdU staining assays. Cell apoptosis was detected by TUNEL staining. Osteogenic differentiation was evaluated by ALP and ARS staining. DCFH-DA staining and γ-H2A.X immunostaining were used to measure intracellular levels of ROS production and DNA damage. Results: Both cell counting and EdU staining assays showed that cell proliferation of BMSCs was significantly reduced upon blue LED irradiation. Furthermore, treatment of BMSCs with LED irradiation was followed by a remarkable increase in apoptosis, indicating that blue LED light induced toxic effects on BMSCs. Likewise, BMSC osteogenic differentiation was inhibited after exposure to blue LED irradiation. Further, blue LED irradiation was followed by the accumulation of ROS

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“…Recently, an efficient and simple method to induce the stem cell differentiation was reported. Lithium (Li), the smallest alkali metal with an atomic weight of 6.9, was found to enhance the efficiency of induced pluripotent stem cell-derived neurospheres (Tafreshi et al 2015), promote the odontoblast differentiation of hair follicle neural crest cells (Shan et al 2015), modulate the proliferation and differentiation of mesenchymal stem cells (Dong et al 2015;Tang et al 2015), prevent neural progenitor cell apoptosis (Cabrera et al 2014). These studies indicated that Lithium may play function in the NSCs' differentiation.…”

Section: Introductionmentioning

confidence: 99%

Lithium chloride promotes neuronal differentiation of rat neural stem cells and enhances neural regeneration in Parkinson’s disease model

Tang

et al. 2017

Cytotechnology

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Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to the advancement in PD therapy. In this study, we used the rat NSCs to elucidate the role of Lithium in the proliferation and differentiation of NSCs by immunostaining against Ki67 and BrdU analysis as well as immunostaining against specific neuronal markers. We concluded that lithium chloride (LiCl) treatment could enhance the proliferation in NSCs and promote the dopaminergic neuronal differentiation of NSCs in vitro. This process was potentially mediated by Wnt signaling pathway. Using the 6-OHDA-induced PD models, we provided evidence to show that LiCl had the capacity to enhance the proliferation in NSCs and differentiation towards dopaminergic neurons in vivo. The beneficial effect of LiCl treatment was further validated by the fact that the motor function as well as learning and memory was improved in the PD models through Rotarod test and Morris water maze analysis. The learning and memory improvement was further supported by the increase in dendrite spine density in PD models receiving LiCl-treated NSCs. Through this study, we concluded that Lithium plays an important role in promoting NSCs' neuronal differentiation in vitro and improving the symptoms of PD models in vivo. It is of great significance that this work showed the potential application of Lithium in the PD therapy in the future.

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Lithium Chloride Modulates Adipogenesis and Osteogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells

Cited by 62 publications

References 33 publications

Lithium enhances alveolar bone formation during orthodontic retention in rats

Lithium enhances alveolar bone formation during orthodontic retention in rats

Effects of Blue Light Emitting Diode Irradiation On the Proliferation, Apoptosis and Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

Lithium chloride promotes neuronal differentiation of rat neural stem cells and enhances neural regeneration in Parkinson’s disease model

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