Lithium alters elicitor-induced H₂O₂ production in cultured plant cells

Abstract

Lithium pollution may seriously influence the metabolic and signalling processes of plants. In the present paper, we investigate the effect of lithium chloride on fungal elicitor-triggered H 2 O 2 generation in Rubia tinctorum L. cell cultures. Our results show that Li + strongly influences elicitor-induced H 2 O 2 formation and time-course in the cells and culture medium. Neomycin, a phospholipase C inhibitor, and 2-APB, an inositol-1,4,5-triphosphate (IP 3 ) receptormediated Ca 2+ release blocker, strongly affected the elicitor-induced H 2 O 2 production and had a similar effect on elicitor-triggered H 2 O 2 formation as Li + . We monitored changes in H 2 O 2 location at subcellular level and our observations confirmed the changes measured by quantitative methods. The obtained results enabled us to deduce that the IP 3 pathway might be involved in the early signalling events leading to the moderation of elicitor-induced reactive oxygen species generation.

Reimagining safe lithium applications in the living environment and its impacts on human, animal, and plant system

“…Orbán and Bóka (2013) reported the formation of H 2 O 2 in rose madder ( Rubia tinctorum ) cell cultures after treatment with 1 and 20 mM LiCl over a varying time period (0–240 min). Importantly, pre-treatment with botrytis caused a large difference in H 2 O 2 formation between 1 mM Li (22 mg g −1 ) and 20 mM Li (28 mg g −1 ) after 240 min [ 73 ]. Exposure of spinach ( Spinacia oleracea ) to 20–80 mg kg −1 LiCl significantly improved the antioxidant activity of superoxide dismutase (19–35%), catalase (27–39%), and ascorbate peroxidase (131–182%), together with an increase in H 2 O 2 release and lipid peroxidation [ 74 ] ( Table 1 ).…”

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

“…Reactive oxygen species (ROS) generated in response to stimuli and during development can also function as signaling molecules in eukaryotes, leading to specific downstream responses. Among these, hydrogen peroxide (H2O2) plays an important role as a key signaling molecule in response to various stimuli and is involved in the accumulation of secondary metabolites (Hao et al 2014, Orbán et al 2013, Wang et al 2005)however hydrogen peroxide from the oxidative burst is not always related to secondary metabolite accumulation. For example, the production of H2O2 has significant positive correlations with both gene expression and taxanes, indicating that the increase in H2O2might be involved in the upregulation of the taxane production in yew under squalestatin, a fungal metabolite.…”