Listeria monocytogenes sequence type 1 is predominant in ruminant rhombencephalitis

Dreyer, Margaux; Aguilar-Bultet, Lisandra; Rupp, Sebastian; Guldimann, Claudia; Stephan, Roger; Schock, A.; Otter, A.; Schüpbach, Gertraud; Brisse, Sylvain; Lecuit, Marc; Frey, Joachim; Oevermann, Anna

doi:10.1038/srep36419

Cited by 86 publications

(146 citation statements)

References 68 publications

(125 reference statements)

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“…With the advances in sequencing technologies, researchers have utilized MLST, MVLST and WGS to study L. monocytogenes strain distribution from animal clinical cases, farms, foods and human clinical cases on a global scale (Dreyer et al . ; Maury et al . ; Steckler et al .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Kotzamanidis¹,

Papadopoulos

Vafeas³

et al. 2019

J Appl Microbiol

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Aims The aim of this study was to evaluate the genetic diversity and resistance phenotypes of Listeria monocytogenes strains isolated from clinical encephalitis cases, and compare this population to isolates derived from tank milk of healthy animals. Methods and Results A total of 57 L. monocytogenes strains isolated from ruminant's listeriosis cases (n = 31) and from tank milk of healthy ruminants (n = 26) were characterized by species PCR, molecular serotyping, PCR detection of virulence genes, pulsed‐field gel electrophoresis and antimicrobial susceptibility testing. All strains possessed inlA, inlC, inlJ, plcA, actA, hlyA and iap virulence‐associated genes while serotyping analysis revealed that they were mainly assigned into IVb group. Genotyping revealed 50 pulsotypes among the 57 strains assigned into seven clusters while indistinguishable pulsotypes between clinical and milk strains were not identified. Resistance of L. monocytogenes isolates to 14–16 antimicrobial agents tested was observed and 23 antimicrobial resistance profiles (ARPs) were defined while no apparent predominant ARP type was observed among isolates. Conclusions Small ruminants are exposed to a broad range of antimicrobial‐resistant as well as genetically diverse strains of L. monocytogenes carrying virulence‐associated genes but not all of them associated with the disease. Pulsed‐field gel electrophoresis analysis suggests that pulsotypes associated with encephalitis are found in farms only in association with listeriosis. Significance and Impact of the Study These findings are valuable in understanding the ecology of this important food‐borne pathogen and creating awareness for the emerging antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

“…Furthermore, recent studies based on epidemiological, clinical and experimental approaches established that L. monocytogenes is a highly heterogeneous species with regards to pathogenicity, and is composed of hypervirulent and hypovirulent clones (Dreyer et al . ; Maury et al . ).…”

Section: Discussionmentioning

confidence: 99%

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Kotzamanidis¹,

Papadopoulos

Vafeas³

et al. 2019

J Appl Microbiol

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“…Previous studies from our group have shown that a ST1 strain from lineage I isolated from bovine rhombencephalitis (JF5203) behaves hyperinvasive compared to lineage II strains including EGD‐e (Dreyer et al, ; Rupp, Bartschi, Frey, & Oevermann, ) suggesting that, besides to the well‐known virulence genes, yet uncharacterized virulence genes may contribute to cellular invasion and virulence of certain strains. Therefore, the aim of this study was to assess the impact of lineage I‐specific virulence candidate genes on the cellular infection process.…”

Section: Introductionmentioning

confidence: 97%

“…This is particularly true for strains from specific sequence types (ST, as determined by multilocus sequence typing (MLST)), namely ST1 and 4. These two ST additionally behave hypervirulent and hyperinvasive in experimental in vivo and in vitro infections (Dreyer et al, ; Guldimann et al, ; Maury et al, ).…”

Section: Introductionmentioning

confidence: 99%

Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins

et al. 2019

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Listeriosis is a severe disease caused by the opportunistic bacterial pathogen Listeria monocytogenes ( L. monocytogenes ). Previous studies indicate that of the four phylogenetical lineages known, lineage I strains are significantly more prevalent in clinical infections than in the environment. Among lineage 1, sequence type (ST1) belongs to the most frequent genotypes in clinical infections and behaves hyperinvasive in experimental in vitro infections compared to lineage II strains suggesting that yet uncharacterized virulence genes contribute to high virulence of certain lineage I strains. This study investigated the effect of four specific lineage I genes encoding surface proteins with internalin‐like structures on cellular infection. CNS derived cell lines (fetal bovine brain cells, human microglia cells) and non‐CNS derived cell lines (bovine macrophage cells, human adenocarcinoma cells) that represent the various target cells of L. monocytogenes were infected with the parental ST1 strain and deletion mutants of the four genes. Despite their association with lineage I, deletion of the four genes investigated did not dampen the hyperinvasiveness of the ST1 strain. Similarly, these genes did not contribute to the intracellular survival and intercellular spread of L. monocytogenes ST1, indicating that these genes may have other functions, either during the infection process or outside the host.

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“…L. monocytogenes is a significant challenge in food production due to its ability to survive under conditions of salinity, alkalinity and temperature stress [1]. Extra chromosomal plasmids are relatively small compared to the bacterial chromosome and often harbor antibiotic resistant genes.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic analysis of Listeria monocytogenes CRISPR

Qu¹,

Shen²,

Xu³

et al. 2018

Oncotarget

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Listeria monocytogenes is a leading causes of death from food-borne pathogens. Bioinformatics approach was applied to investigate the features of L. monocytogenes CRISPR structure and the relationship between CRISPR and plasmid transposase content. Among 93 L. monocytogenes genomes, 95 confirmed CRISPR structure loci were identified and classified into 5 groups based on repeat size. RNA secondary structure and minimum free energy indicated that the secondary structure of Group 5 (36 bp) was more stable than other groups. Type I-B or II-A Cas genes were found in 36 strains, and the CRISPR-Cas system of type I-B was more conserved than type II-A. Furthermore, CRISPR loci affected the enzyme transposase content of L. monocytogenes plasmid. This study examined the diversity of the CRISPR-Cas system in L. monocytogenes, classified CRISPR structure and repeats, and demonstrated the influence of the CRISPR-Cas system on the number of transposase in plasmid.

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Listeria monocytogenes sequence type 1 is predominant in ruminant rhombencephalitis

Cited by 86 publications

References 68 publications

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins

Bioinformatic analysis of Listeria monocytogenes CRISPR

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