Listeria monocytogenes: Occurrence in beef and identification of the main contamination points in processing plants

Barros, Márcia de Aguiar Ferreira; Nero, Luís Augusto; Silva, Lívia C; d’Ovídio, Loredana; Monteiro, Fabrício Amorim; Tamanini, Ronaldo; Fagnani, Rafael; Hofer, Ernesto; Beloti, Vanerli

doi:10.1016/j.meatsci.2007.01.016

Cited by 57 publications

(33 citation statements)

References 26 publications

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“…Similar observations were also noted during other conducted studies where the strains in the meat processing environment survived even for a 3-year period (8,10,12,13). It has been also proved that the improvement of hygiene procedures reduced the prevalence and spread of L. monocytogenes in meat products intended to retail sale (1,11).…”

Section: Discussionsupporting

confidence: 85%

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Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Dmowska

Wieczorek

Lynch

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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A total of 135 L. monocytogenes strains isolated from slaughtered cattle and beef meat were tested by the pulsed field gel electrophoresis (PFGE). The AscI restriction analysis revealed a genetic heterogeneity among investigated isolates since 31, 9, and 35 profiles were distinguished among hide, carcass, and meat strains, respectively. The PFGE profiles of the isolates were also analysed in relation to serotypes, virulence genes, and antimicrobial resistance. It was shown that strains displaying the same PFGE type were of the same serotype while correlation between pulsotype and antimicrobial resistance was poor. The obtained results suggest that a cross-contamination between bovine hides and carcasses may occur during the slaughter process. Moreover, identification of identical PFGE types among L. monocytogenes found during a study period may suggest a common source of contamination or presence of persistent strains able to survive for a long time. These results emphasise the importance of molecular subtyping methods, including PFGE, in monitoring and tracking pathogen contamination along food chain.

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Section: Discussionsupporting

confidence: 85%

“…Several studies demonstrated that L. monocytogenes is present along beef chain (1,4,14,17,18). There are various routes of introducing pathogen into food-processing environments, e.g.…”

mentioning

confidence: 99%

Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Dmowska

Wieczorek

Lynch

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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Section: Introductionmentioning

confidence: 99%

“…Nevertheless, previous studies described a high ocurrence of 4b serotype in human and foods specimens [14][15][16][17][18]. In an extensive study, Hofer et al [14] observed the prevalence of the 4b serotype in meat products and fish from different sources in Brazil, followed by 1/2a and 1/2b serotypes. Due to the importance of the 4b serotype in the epidemiological scenario of listeriosis, the aim of this study was to evaluate the presence of major epidemic clones (ECI and ECII), virulence markers and genetic relatedness among a selected group of L. monocytogenes serotype 4b strains isolated from human listeriosis and meat-based foods in Brazil.…”

Section: Introductionmentioning

confidence: 99%

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Barbosa

Cerqueira

Rusak

et al. 2015

J Infect Dev Ctries

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Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECIIpositive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.

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“…3,4 The occurrence of L. monocytogenes in fresh beef was also established by several studies. [5][6][7] L. monocytogenes is a ubiquitous organism, able to survive and multiply at refrigeration temperatures, in up to 12-13% salt, while certain strains may grow in water activity down to 0.9 and a pH value down…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of barrier film with a cellulose coating that carries nisin blends for the inhibition of listeria monocytogenes

et al. 2010

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SUMMARYThe objective of this study was to determine the effectiveness of two nisin blend antimicrobial agents (Guardian NR 250, Novagard CBI) incorporated into a cellulose coating that was applied onto a barrier fi lm against Listeria monocytogenes. The minimum inhibitory concentration (MIC) of the agents in solution against L. monocytogenes was found to be 2.74 mg/ml for the two nisin blends. The concentrations tested for both nisin blend treatments were 5.49, 10.9, 16.4 and 21.9 mg/ml. Guardian NR 250 resulted in wider zones of inhibition compared to the Novagard CBI at all levels tested. The MIC for Guardian NR 250 in the fi lm was 5.49 mg/ml. Films containing Novagard CBI did not show any antimicrobial activity. A food challenge study was conducted using the fi lm containing Guardian NR 250 at levels of 5.49 and 21.9 mg/ ml. Inoculated fresh beef cubes were individually packaged with pre-made barrier fi lm pouches that had an interior cellulose coating containing the antimicrobial agent and stored at 4°C for 36 days. Bacterial colonies were enumerated every 6 days on modifi ed Oxford agar. There was no signifi cant difference in the L. monocytogenes population between two levels of Guardian NR 250 throughout the study. There was statistically signifi cant inhibition of L. monocytogenes for both levels of Guardian NR 250 during 18-30 days of storage compared to a control fi lm without the antimicrobial agent.

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Listeria monocytogenes: Occurrence in beef and identification of the main contamination points in processing plants

Cited by 57 publications

References 26 publications

Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Effectiveness of barrier film with a cellulose coating that carries nisin blends for the inhibition of listeria monocytogenes

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