Listeria monocytogenes inhibition by defatted mustard meal-based edible films

Antimicrobial edible defatted soybean meal-based films incorporating the lactoperoxidase system

“…A disk-covering test was conducted following the method of Lee, Noh, and Min (2012). The inoculum (100 mL) was spread on the surface of the TSA plate to produce a lawn of approximately 4 log CFU/cm 2 .…”

Edible films and coatings: Sources, properties and application

“…There are several categories of antimicrobials that can be potentially incorporated into edible films and coatings, including organic acids (acetic, benzoic, lactic, propionic, sorbic), fatty acid esters (glyceryl monolaurate), polypeptides (lysozyme, peroxidase, lactoferrin, nisin), plant essential oils (cinnamon, oregano, lemongrass), nitrites and sulphites, among others (Franssen & Krochta, 2003). Active function of the edible film and coating system protects food products from oxidation and microbial spoilage, resulting in quality improvement (organoleptic preference and the visual perception of quality) and enhanced safety Lee et al, 2012).…”

Prediction of the coating requirements for smoked salmon protection against Listeria monocytogenes using a defatted mustard meal-based antimicrobial edible film containing thiocyanates

“…The meal was ground in a Scienceware Micro mill (Bel-Art Products, Pequannock, NJ, USA) and then sieved to prepare powder (<300 mm). The antimicrobial DMM film was prepared by following the method of Lee et al (2012). Briefly, 14 g DMM powder was mixed with 184 g water for 30 min and then 2, 4, and 6 g glycerol plasticizer (G9012, SigmaeAldrich Co. Ltd., St. Louis, MO, USA) was mixed in for 20 min to prepare DMM films with the weight ratios of glycerol-DMM of 1:7, 2:7, and 3:7, respectively.…”

“…The pH of the homogenate was adjusted to 6.5 with 0.1 and 1 mol equi/L NaOH (Fisher Scientific Inc., Fair Lawn, NJ, USA). Then the solution was incubated at 37 C for 48 h to facilitate formation of SCN À in the DMM film-forming solution by myrosinase, an indigenous enzyme in DMM (Lee et al, 2012). The incubated solution was maintained at 90 C for 30 min in a water bath to inactivate myrosinase in the solution and then cooled on ice.…”

“…An antimicrobial biopolymer film made from the industry byproduct defatted mustard meal (DMM film) was developed previously without incorporating external antimicrobials and demonstrated feasibility to control L. monocytogenes already present on food products, as well as for inhibiting their growth on filmwrapped or coated food products (Lee, Noh, & Min, 2012). Mustard contains organic compounds called glucosinolates.…”