Listeria monocytogenes in raw milk, milking equipment and dairy workers: Molecular characterization and antimicrobial resistance patterns

Tahoun, Asmaa B.M.B.; Elez, Rasha M.M. Abou; Abdelfatah, Eman Nabil; Elsohaby, Ibrahim; El-Gedawy, Attia; Elmoslemany, Ahmed

doi:10.1016/j.jgar.2017.07.008

Cited by 55 publications

(45 citation statements)

References 31 publications

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“…Gelbícová and Karpísková [16] reported that macrorestriction analysis revealed a considerable heterogeneity of profiles, with nine different pulsotypes being detected in raw milk from the Czech Republic. In line with our results, Tahoun et al [17] reported high genetic relatedness between L. monocytogenes isolates recovered from raw milk, milk equipment, and farm workers. Castro et al [18] have found only two pulsotypes that occurred in multiple samples of raw milk.…”

Section: Discussionsupporting

confidence: 92%

Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia

et al. 2019

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The aim of the present study was to evaluate the persistence of Listeria monocytogenes in raw milk from vending machines, based on culture and molecular detection of pathogen and Pulsed-Field Gel Electrophoresis typing. From December 2015 to January 2017, a total of 319 milk samples from 36 vending machines were examined for the presence and count of L. monocytogenes by reference methods ISO 11290:1 and ISO 11290:2. Molecular detection of pathogens was performed by loop-mediated isothermal DNA amplification (LAMP) coupled with bioluminescence (Molecular Detection Assay). L. monocytogenes was detected by MDA in 14 milk samples (4.38%) from four farms, compared to eight positive samples (2.5%) retrieved by a reference ISO method. Cultivable L. monocytogenes isolates were subjected to Pulsed-Field Gel Electrophoresis typing and pulsotypes were compared with those obtained during the previous survey in Croatia (2014‒2015). It was found that identical PFGE patterns of L. monocytogenes occur in milk samples of the same producer over a three-year period, indicating the persistence of pathogens in raw milk vending machines. The results obtained support the need for more effective control of milk in the entire food chain.

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Section: Discussionsupporting

confidence: 92%

Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia

et al. 2019

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“…103 – 107 The high resistance of E. coli O157:H7 , L. monocytogenes , and K. pneumoniae to tetracycline correlated to the extensive use of tetracycline in dairy farms for treatment of infectious diseases. 108 In the current study, all strains had a MAR index which ranged between 0.44–0.88, since it is well known that isolates which display a MAR index less than 0.2 are supposed to come from antibiotic-rare used sources. 109 These data confirm that the usage of antimicrobial agents is highly linked to the evolution risk of MDR bacteria.…”

Section: Discussionmentioning

confidence: 72%

<p>Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt</p>

El-Gohary¹,

Abdel-Hafez²,

Zakaria³

et al. 2020

IDR

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The last few decades have witnessed a rapid and global increase in multidrugresistant bacteria (MDR) emergence. Methods: The aim of the current study is to isolate the most common MDR bacteria from dairy farms and beef slaughterhouses followed by evaluation of their antimicrobial resistance pattern and assessment of the antibacterial activity of AgNPs-H 2 O 2 as an alternative to conventional antibiotics. In this regard, 200 samples were collected from two dairy farms and one beef slaughterhouse located in Dakhliya Governorate, Egypt. Results: Interestingly, out of 120 collected samples from dairy farms, the prevalence of the isolated strains was 26.

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“…The results were similar to previous reports from China, the United States, Ireland, and Poland (Wieczorek et al, 2012; Shen et al, 2013; Chen et al, 2015; Khen et al, 2015; Wieczorek and Osek, 2017) and consistent with the general thought that the Listeria genus is naturally susceptible to ampicillin, penicillin, gentamicin and erythromycin, which are usually active against Gram-positive bacteria (Wang et al, 2013; Chen et al, 2015; Wu et al, 2015, 2016). On the other hand, L. monocytogenes strains isolated from raw milk, milk equipment and farm workers in Egypt showed lower susceptibility rates to gentamicin (19.0%), rifampin (0%) and ciprofloxacin (42.9%) (Tahoun et al, 2017). The most prevalent detected antibiotic resistance type was resistance to oxacillin.…”

Section: Resultsmentioning

confidence: 99%

“…Breakpoints for Penicillin and Ampicillin were found in CLSI documents M45-A3 (Clinical and Laboratory Standards Institute, 2015). Since there is no relevant criteria for Erythromycin, Clindamycin, Quinupristin/dalfopristin, Vancomycin, Tetracycline, Gentamicin, Rifampin, Levofloxacin, Ciprofloxacin, Gatifloxacin, and Oxacillin, the susceptibility results for these antibiotics were interpreted based on the breakpoints of Staphylococcus spp (Clinical and Laboratory Standards Institute, 2017) as reported previously (Conter et al, 2009; Lungu et al, 2011; Chen et al, 2015; Khen et al, 2015; Tahoun et al, 2017). L. monocytogenes isolates resistant to three or more types of antibiotics belonging to different antibiotic classes were defined as multi-drug resistant (Magiorakos et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Prevalence, Genotypic Characteristics and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Bulk in Zhejiang Province, China

et al. 2019

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Listeria monocytogenes is an important foodborne pathogen causing public concern. A total of 3354 retail foods in bulk were sampled and screened for L. monocytogenes . Seventy-three (2.2%) samples including 21 ready-to-eat (RTE) foods and 52 raw foods were confirmed positive for L. monocytogenes . Sushi and salmon sashimi occupied the top two slots in RTE foods with relatively high presence rate of 12.9 and 6.9%, respectively. Meanwhile, L. monocytogenes was found to be distributed unequally in raw foods; the presence rates in raw meat (3.5%) and poultry (3.8%) were significantly higher than that in raw seafood (1.3%). Notably, L. monocytogenes was not detected in raw freshwater food. The L . monocytogenes isolates belonged to four serotypes, 1/2a, 1/2b, 1/2c, and 4b, with the most prevalent serotype being 1/2a (47.9%). Eighteen sequence types (STs) and eighteen virulence types (VTs) containing four newly assigned VTs (VT180, VT181, VT182, and VT183) were determined via multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MVLST). Among the 73 L. monocytogenes isolates, 23 (31.5%) belonged to epidemic clones (ECs) including ECI, ECIV, ECV, ECVI, ECVIII and ECXI among which ECV was predominant. Antibiotic susceptibility tests revealed a high resistance rate (11.0%) to tetracycline. Moreover, we identified the distribution patterns of virulence genes of four Listeria pathogenicity islands (LIPI) in L. monocytogenes isolates. prfA , hly , plcA , plcB , mpl , actA genes in LIPI-1 and inlA , inlB , inlC , inlJ genes in LIPI-2 were detected in approximately all L. monocytogenes isolates. The distribution of both LIPI-3 genes and LIPI-4 genes exhibited association with lineage and ST. LIPI-4 genes were present exclusively in ST87 isolates. Relatedness analysis revealed the absence of distinct association between STs, ECs, LIPI-3 and LIPI-4 distribution and specific food groups. This study provided fundamental data for Chinese food safety authorities to grasp the contamination status of L. monocytogenes in foods, assess the potential risk of this pathogen and further address the safety issue of retail foods in bulk in China.

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Listeria monocytogenes in raw milk, milking equipment and dairy workers: Molecular characterization and antimicrobial resistance patterns

Cited by 55 publications

References 31 publications

Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia

Detection and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes Isolates from Milk Vending Machines in Croatia

<p>Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt</p>

Prevalence, Genotypic Characteristics and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Bulk in Zhejiang Province, China

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