Listeria monocytogenes impairs enzymes of the phosphotransfer network and alters antioxidant/oxidant status in cattle brain structures

Jaguezeski, Antonise M.; Baldissera, Matheus D.; Rhoden, Leandro A.; Gomes, Teane Milagres Augusto; Mendes, Ricardo E.; Bottari, Nathieli B.; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina; Stefani, Lenita M.; Giongo, Janice Luehring; Vaucher, Rodrigo de Almeida; Silva, Aleksandro Schafer Da

doi:10.1016/j.micpath.2018.08.044

Cited by 10 publications

(11 citation statements)

References 38 publications

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“…Sensitivity of IHC and IF were calculated separately in two animal groups: naturally infected and experimentally infected. As gold standard tests, qPCR of frozen tissues was used for experimentally infected animals (JAGUEZESKI et al, 2018;JAGUEZESKI et al, 2020), whereas histopathological lesion of neurologic listeriosis was used for naturally infected animals.…”

Section: Discussionmentioning

confidence: 99%

“…other 22 tissue samples were obtained from five cattle (n = 20) and two gerbils (n = 2) experimentally infected with L. monocytogenes ATCC 7644 (JAGUEZESKI et al, 2018;JAGUEZESKI et al, 2020). Gerbils were infected with 5 x 10 8 CFU of bacteria per animal by the oral route and were euthanized at 12 days post infection (dpi), which were observed multifocal hepatic microgranulomas (JAGUEZESKI et al, 2020).…”

Section: Sample Selectionmentioning

confidence: 99%

“…Gerbils were infected with 5 x 10 8 CFU of bacteria per animal by the oral route and were euthanized at 12 days post infection (dpi), which were observed multifocal hepatic microgranulomas (JAGUEZESKI et al, 2020). Cattle were infected with 1.32 x 10 10 CFU of bacteria per animal by the oral mucosa after scarification and were euthanized at 14 dpi (JAGUEZESKI et al, 2018). Fragments of liver, jejunum, oral mucosa, brainsteam and/or cerebrum from these animals were previously tested by qPCR (JAGUEZESKI et al, 2018;JAGUEZESKI et al, 2020) and only positive samples were selected for this study.…”

Section: Sample Selectionmentioning

confidence: 99%

“…Cattle were infected with 1.32 x 10 10 CFU of bacteria per animal by the oral mucosa after scarification and were euthanized at 14 dpi (JAGUEZESKI et al, 2018). Fragments of liver, jejunum, oral mucosa, brainsteam and/or cerebrum from these animals were previously tested by qPCR (JAGUEZESKI et al, 2018;JAGUEZESKI et al, 2020) and only positive samples were selected for this study. Additionally, fragments of liver, jejunum, brain steam and cerebrum from uninfected bovines (control group) were used a negative control.…”

Section: Sample Selectionmentioning

confidence: 99%

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Comparison between immunofluorescence and immunohistochemistry for Listeria monocytogenes detection in formalin-fixed paraffin-embedded tissues

et al. 2022

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Listeria monocytogenes is a bacterium that infect humans and animals and causes a zoonotic disease characterized by encephalitis, septicemia or abortion. In addition, listeriosis leads to significant economic losses due to animal death and sacrifice. This research compared the technique of immunofluorescence (IF) and immunohistochemistry (IHC) for the diagnosis of L. monocytogenes in formalin-fixed and paraffin-embedded (FFPE) tissues. A total of 30 tissue blocks from 15 animals with history and/or lesions compatible with listeriosis were selected. For both IHC and IF, the same diluted (1:200) polyclonal primary antibody was used against L. monocytogenes serotypes 1 and 4. For IHC, a polymer secondary antibody conjugated to peroxidase (HRP) was used. For IF, samples were incubated with a fluorescein-labeled anti-rabbit IgG secondary antibody. Each sample was classified according to the presence and percentage of immunolabeling area. From 30 samples, 10 were positive at least for one technique, whereas eight samples were positive for both IHC and IF with similar score. There was strong immunolabeling in tissue samples from bovines experimentally infected with L. monocytogenes ATCC 7644, as well as in nervous tissues from naturally infected ruminants. Additionally, IF did not show any difference in sensitivity when compared to IHC. Using processed biological materials for IF, instead of fresh tissues, is a quite unique technique, since there are few protocols described. Therefore, this study demonstrated that both techniques are efficient to detect L. monocytogenes in FFPE tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Selectionmentioning

confidence: 99%

Section: Sample Selectionmentioning

confidence: 99%

Section: Sample Selectionmentioning

confidence: 99%

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Comparison between immunofluorescence and immunohistochemistry for Listeria monocytogenes detection in formalin-fixed paraffin-embedded tissues

et al. 2022

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“…The harmful effects of free radicals are eliminated by antioxidants. However, when the antioxidant defense system is insufficient or when oxidants called ROS and RNS are produced in excess, the cells are damaged due to oxidative stress (Abd Ellah et al 2009;Jaguezeski et al 2018). Damage to the cell membrane is caused by ROS.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Vitamin C on Oxidant and Antioxidant Parameters in Anthrax Vaccine Administered Cattle

Doğan

Merhan

Erdağ

et al. 2021

Van Veterinary Journal

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In this study, the effects of Vitamin C on oxidative stress in anthrax vaccinated cattle were investigated. The research was performed on 40 healthy cattle without any race and gender difference, and aged 6-8 months that were not anthrax vaccinated. The cattle were divided into four equal groups. The first group was determined as the control group. 1 ml anthrax vaccine was administered to the second group, 1 ml anthrax vaccine and 5 mg/kg dose of vitamin C was administered to the third group, and vitamin C was administered to the fourth group at a dose of 5 mg/kg. Blood collection were collected and serum samples were extracted just before the vaccination and drug administration (0 th day) and on the 2 nd , 14 th and 28 th days after the administration. Nitric Oxide (NO), Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (CAT) levels of serum samples were evaluated. It was found that the MDA and NO levels significantly increased (P<0.05), and the amount of SOD and CAT decreased in the vaccine group on the 2 nd , 14 th and 28 th days compared to the control group. It was determined that the MDA and NO levels in the vaccine-vitamin groups on the 2 nd , 14 th and 28 th days decreased insignificantly (P> 0.05) compared to the vaccine group. It can be asserted that the use of vitamin C in combination with anthrax vaccine in cattle would be beneficial in terms of reducing oxidative stress.

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Saprolegnia parasitica impairs branchial phosphoryl transfer network in naturally infected grass carp (Ctenopharyngodon idella): prejudice on bioenergetic homeostasis

et al. 2019

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Listeria monocytogenes impairs enzymes of the phosphotransfer network and alters antioxidant/oxidant status in cattle brain structures

Cited by 10 publications

References 38 publications

Comparison between immunofluorescence and immunohistochemistry for Listeria monocytogenes detection in formalin-fixed paraffin-embedded tissues

Comparison between immunofluorescence and immunohistochemistry for Listeria monocytogenes detection in formalin-fixed paraffin-embedded tissues

The Effect of Vitamin C on Oxidant and Antioxidant Parameters in Anthrax Vaccine Administered Cattle

Saprolegnia parasitica impairs branchial phosphoryl transfer network in naturally infected grass carp (Ctenopharyngodon idella): prejudice on bioenergetic homeostasis

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