Listeria monocytogenes Biofilms in the Wonderland of Food Industry

Colagiorgi, Angelo; Bruini, Ilaria; Ciccio, Pierluigi Aldo Di; Zanardi, Emanuela; Ghidini, Sergio; Ianieri, Adriana

doi:10.3390/pathogens6030041

Cited by 170 publications

(132 citation statements)

References 68 publications

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“…Listeria monocytogenes biofilms contribute to persistence in the food processing environment as biofilms can be formed on many different surfaces and serve as a source of subsequent contamination (Colagiorgi et al . ). Another aspect of L. monocytogenes physiology which may contribute to persistence in the food processing environment is the ability of the bacteria to enter the VBNC state.…”

Section: Potential Survival Mechanisms Of L Monocytogenes In the Foomentioning

confidence: 97%

“…In addition, SigB has been shown to be activated in biofilms and appears to be an essential gene for the formation of biofilms with increased resistance to disinfectants in L. monocytogenes (van der Veen and Abee 2010). Listeria monocytogenes biofilms contribute to persistence in the food processing environment as biofilms can be formed on many different surfaces and serve as a source of subsequent contamination (Colagiorgi et al 2017). Another aspect of L. monocytogenes physiology which may contribute to persistence in the food processing environment is the ability of the bacteria to enter the VBNC state.…”

Section: Harbourage Sites and Persistent Strains In Food Processing Fmentioning

confidence: 99%

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Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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Listeria monocytogenes is an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processed on a large scale before reaching the consumer through various products such as sandwiches and prepared salads. From farm to fork, the fresh leafy produce supply chain (FLPSC) is complex and contains a diverse range of environments where L. monocytogenes is sporadically detected during routine sampling of produce and processing areas. This review describes sources of the bacteria in the FLPSC and outlines the physiological and molecular mechanisms behind its survival in the different environments associated with growing and processing fresh produce. Finally, current methods of source tracking the bacteria in the context of the food supply chain are discussed with emphasis on how these methods can provide additional, valuable information on the risk that L. monocytogenes isolates pose to the consumer.

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Section: Potential Survival Mechanisms Of L Monocytogenes In the Foomentioning

confidence: 97%

Section: Harbourage Sites and Persistent Strains In Food Processing Fmentioning

confidence: 99%

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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“…In the Disc Diffusion study, the anti-microbial activity of echinocystic acid (2) was evaluated against all the Gram-positive Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus and Gram-negative Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa bacteria ( Table 1) [8][9][10][11]. It showed significant anti-microbial potential against the Gram-positive Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus and Gram-negative Salmonella typhimurium bacteria ( Figure 1).…”

Section: Resultsmentioning

confidence: 99%

“…In every two hours interval aliquot of culture (both treated and untreated) was withdrawn aseptically diluted serially with sterilized distilled water and plated into nutrient agar medium, incubated at 37°C for another 24 hrs. The colonies appeared were counted per ml of culture broth [7][8][9][10][11].…”

Section: Anti-microbial Mode Of Action Of Echinocystic Acid (2) On Grmentioning

confidence: 99%

Anti-microbial and Anti-cancer Properties of Echinocystic Acid Extracted from Luffa cylindrica

Garai¹,

Ghosh²,

Pp³

et al. 2018

J Food Process Technol

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Triterpenoids are the most ubiquitous non-steroidal secondary metabolites in terrestrial and marine flora and fauna occurring in the free form as well as in the forms of ether, ester and glycosides. The diverse structural types of triterpenoids with useful biological activities, identification, biosynthesis for feed industry increases interest in triterpenoids. The widely occurring plant Luffa cylindrica was investigated for active triterpenoid sapogenins. The triterpenoid sapogenins were isolated by solvent extraction followed by chromatography and were defined by newer spectroscopic methods. These triterpenoid sapogenins from Luffa cylindrica were isolated and characterized. The anti-microbial and anti-cancer properties of echinocystic acid will be discussed.

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“…Surfaces with biofilms of L. brevis did not show any positive reaction because this microorganism is catalase negative (Whittenbury, 1964) and no positive action was expected. In food industry environment, the non-detection of negative catalase microorganisms would not be a problem because biofilms are formed by mixed species (Colagiorgi et al, 2017;Elias & Banin, 2012;Phillips, 2016) and therefore the use of the biodetector would show microbial activity. Biofilms formed by mixed species constitutes a general adaptation of foodborne pathogens and microbiota associated to the food-processing environment for extended survival in their food niche (Jahid & Ha, 2014).…”

Section: Detection Of Biofilms Using Dem and The Different Biodetectomentioning

confidence: 99%

Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

Ripolles‐Avila

Ríos-Castillo

Jerez

2018

CyTA - Journal of Food

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Detection of biofilms on food-contact surfaces is essential for establishing sanitization procedures and avoiding microbial contamination of food products. The objective of our study was to develop a peroxide biodetector, to detect and reveal the presence of biofilms. A positive reaction was observed when biodetector bubbles were formed on stainless steel and polypropylene surfaces. A range of microorganisms able to form biofilms was evaluated. Catalase-positive bacteria: Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium and Cronobacter sakazakii were employed as well as the catalase-negative bacterium: Lactobacillus brevis. With the exception of C. sakazakii, all catalase-positive foodborne pathogens forming the biofilms showed a positive detection by the biodetector, being 10 4 CFU cm −2 the minimum microbial load detected. The strongest positive reaction was for P. aeruginosa. Results demonstrated the potential of this biodetector to detect biofilms, particularly when used as a tool in the food industry.Desarrollo de un biodetector a base de peróxido para la detección directa de biofilms producidos por bacterias catalasa positivas en superficies de contacto con alimentos RESUMEN La detección de biofilms en superficies de contacto con alimentos es, hoy en día, esencial para establecer procedimientos de limpieza y desinfección eficaces y evitar la contaminación microbiana de productos alimenticios. El objetivo del presente estudio fue el desarrollo de un biodetector a base de peróxido, para detectar y revelar la presencia de biofilms mediante la formación de burbujas en superficies de acero inoxidable y polipropileno. Diferentes microorganismos fueron empleados en el estudio, entre ellos, bacterias catalasa positivas (Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium y Cronobacter sakazakii) y una bacteria catalasa negativa (Lactobacillus brevis). Con la excepción de C. sakazakii, el resto de patógenos mostraron una detección positiva por el biodetector, siendo 10 4 CFU cm −2 la mínima carga microbiana detectada. La reacción positiva más abundante fue para P. aeruginosa. Los resultados obtenidos demostraron el gran potencial que tiene el biodetector para la detección de biofilms, especialmente cuando éste se usa como herramienta en la industria alimentaria. ARTICLE HISTORY

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Listeria monocytogenes Biofilms in the Wonderland of Food Industry

Cited by 170 publications

References 68 publications

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Anti-microbial and Anti-cancer Properties of Echinocystic Acid Extracted from Luffa cylindrica

Development of a peroxide biodetector for a direct detection of biofilms produced by catalase-positive bacteria on food-contact surfaces

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