Listeria costaricensis sp. nov.

Abstract

A bacterial strain isolated from a food processing drainage system in Costa Rica fulfilled the criteria as belonging to the genus Listeria, but could not be assigned to any of the known species. Phylogenetic analysis based on the 16S rRNA gene revealed highest sequence similarity with the type strain of Listeria floridensis (98.7 %). Phylogenetic analysis based on Listeria core genomes placed the novel taxon within the Listeria fleishmannii, L. floridensis and Listeria aquatica clade (Listeria sensu lato). Whole-genome sequence analyses based on the average nucleotide blast identity (ANI<80 %) indicated that this isolate belonged to a novel species. Results of pairwise amino acid identity (AAI>70 %) and percentage of conserved proteins (POCP>68 %) with currently known Listeria species, as well as of biochemical characterization, confirmed that the strain constituted a novel species within the genus Listeria. The name Listeria costaricensis sp. nov. is proposed for the novel species, and is represented by the type strain CLIP 2016/00682 (=CIP 111400=DSM 105474).

Emergence of Antibiotic Resistance in Listeria monocytogenes Isolated from Food Products: A Comprehensive Review

“…These species are commonly isolated from intestinal tract of symptom‐free animals and animal‐origin food products (Chiara et al., ; Orsi & Wiedmann, ; Schardt et al., ). The second group is the “ Listeria sensu lato ” clade and consists of 12 species including: L. weihenstephanensis, L. fleischmannii, L. rocourtiae , L. booriae, L. riparia, L. grayi , L. floridensis , L. aquatica , L. newyorkensis , L. cornellensis , L. grandensis , and L. costaricensis , which have been isolated from the environment or food matrices, but they are unable to colonize mammalian hosts (Bertsch et al., ; Chiara et al., ; den Bakker et al., ; Graves et al., ; Lang Halter, Neuhaus, & Scherer, ; Leclercq et al., ; Núñez‐Montero et al., ; Orsi & Wiedmann, ; Orsi, den Bakker, & Wiedmann, ; Schardt et al., ; Weller, Andrus, Wiedmann, & den Bakker, ). Metabolically, all Listeria spp.…”

“…are positive for the catalase test (except L. costaricensis ) and for the production of acid from N ‐acetylglucosamine, arbutin, salicin, D ‐fructose, amygdalin, aesculin‐ferric citrate, cellobiose, and D ‐mannose. However, all species fail to reduce nitrite, and cannot produce acid from raffinose, glycogen, methyl β ‐ d ‐xylopyranoside, d ‐arabinose, d ‐adonitol, and potassium 2‐ketogluconate (Núñez‐Montero et al., ; Orsi & Wiedmann, ; Weller et al., ). Other differences in biochemical reactions between L. monocytogenes and other members of Listeria are illustrated in Table .…”

Receptor-targeted engineered probiotics mitigate lethal Listeria infection

“…In the genus, Listeria , Lm , and L. ivanovii are pathogenic while L. innocua (Lin) and 14 other Listeria species are nonpathogenic 25 . Lm is well adapted to survive in the harsh environment of the intestine 26 , 27 and overcomes gut-associated innate defense 28 to cross the intestinal epithelial barrier.…”

Optimización de temperatura de anillamiento de PCR multiplex para la detección de Listeria monocytogenes

“…Desde el año 2015, en el Tecnológico de Costa Rica, se han realizado distintas investigaciones relacionadas a la detección de Listeria spp en Costa Rica. Un resultado de estos proyectos fue el descubrimiento de una nueva especie a la cual se le nombró Listeria costaricensis [9]. Estos proyectos han sido financiados por la Vicerrectoría de Investigación y Extensión (VIE) y ha contado con la participación de Investigadores de las Escuelas de Biología y Química, además, del apoyo del Instituto Pasteur en Francia.…”