Liquid–liquid equilibria for systems glycerol+sardine oil+tert-alcohols

Solaesa, Ángela García; Bucio, Silvia Liliana; Sanz, Marı́a Teresa; Beltrán, Sagrario; Rebolleda, Sara

doi:10.1016/j.fluid.2013.07.026

Cited by 6 publications

(8 citation statements)

References 23 publications

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“…Different molar ratios of substrates and enzyme dosages were studied. The amount of TP added was calculated on the basis of previous studies on LLE (Solaesa, Bucio, Sanz, Beltrán, & Rebolleda, 2013). It was fixed at mass ratio of 1.5:1 (TP:substrates).…”

Section: Enzymatic Glycerolysis Of Sardine Oilmentioning

confidence: 99%

“…Moreover, Candida antarctica lipase B does not act on tertiary alcohols due to steric hindrance (Yang, Rebsdorf, Engelrud, & Xu, 2005). The liquid-liquid equilibria (LLE) of sardine oil + glycerol + tertalcohols have been determined previously (Solaesa, Bucio, Sanz, Beltrán, & Rebolleda, 2013). LLE data help to minimize the amount of solvent needed to create a homogeneous reaction system, which leads to an improvement in mass transfer, obtaining high reactions yields in short reaction time.…”

Section: Introductionmentioning

confidence: 99%

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Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

et al. 2016

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Section: Enzymatic Glycerolysis Of Sardine Oilmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

et al. 2016

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“…The amount of water added to the system varied in the range of 4 to 30 wt%, based on glycerol weight, in the reaction medium. The amount of tert-alcohol added was calculated on the basis of previous studies on liquid liquid equilibrium for the glycerolysis systems of sardine oil with the two tert-alcohols as reaction medium (Solaesa et al, 2013). Table 2 shows the amount of solvent added in other glycerolysis systems found in the literature to avoid mass transfer limitation due to the immiscibility of oil and glycerol.…”

Section: Enzymatic Glycerolysis Of Sardine Oilmentioning

confidence: 99%

“…The analysis of remaining glycerol was performed using High-Temperature Gas Chromatography (HT-GC). The method and the calibration procedure were previously developed (Solaesa et al, 2013).…”

Section: Determination Of Reaction Productsmentioning

confidence: 99%

“…Moreover, these alcohols do not involve reactions with fatty acids, most likely due to their tertiary structure, which exerts strong steric hindrance to the enzyme. The liquid-liquid equilibrium (LLE) data of these systems (sardine oil + glycerol + tert-alcohols) have been determined in a previous work (Solaesa et al, 2013). LLE data help to optimize the amount of solvent needed to create a homogeneous reaction system, since by increasing the amount of solvent in the reaction medium, the concentration of substrates at the interface decreases, leading to lower reaction rates.…”

Section: Introductionmentioning

confidence: 99%

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Glycerolysis of sardine oil catalyzed by a water dependent lipase in different tert-alcohols as reaction medium

Solaesa¹,

Sanz²,

Melgosa³

et al. 2015

Grasas y Aceites

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SUMMARY:The production of monoacylglycerol rich in polyunsaturated fatty acids (PUFA) via enzymatic glycerolysis of sardine oil in a homogeneous system was evaluated. Reactions were conducted in two different tert-alcohols. Based on the phase equilibrium data, the amount of solvent added to create a homogeneous system has been calculated and optimized. The immobilized lipase used in this work was Lipozyme RM IM from Rhizomucor miehei, a water dependent lipase. The amount of water added as well as other reaction parameters were studied to evaluate the optimum conditions for monoacylglycerol obtencion. An initial reactant mole ratio glycerol to sardine oil 3:1, 12 wt% of water based on glycerol content and 10 wt% of lipase loading (based on weight of reactants), achieved a MAG yield of around 70%, with nearly 28 wt% PUFA, with low free fatty acid content (lower than 18 wt%).KEYWORDS: Fish oil; Glycerolysis; Lipase; Monoacylglycerol; Rhizomucor miehei; Tert-alcohols RESUMEN: Glicerolisis de aceite de sardina catalizada por una lipasa dependiente de agua en diferentes tert-alcoholes como medio de reacción. En este trabajo se ha estudiado la producción de monoacilglicéridos, ricos en ácidos grasos poliinsaturados (AGPI), mediante glicerolisis enzimática de aceite de sardina. La reacción se ha llevado a cabo en dos tert-alcoholes para conseguir de esta forma un medio homogéneo de reacción. La cantidad de disolvente añadida al medio de reacción se ha optimizado y calculado en base al equilibrio de fases de los componentes del sistema. La lipasa empleada como biocatalizador ha sido la enzima inmovilizada Lipozyme RM IM de Rhizomucor miehei, una lipasa dependiente de agua. Se ha estudiado el efecto de distintos parámetros cinéticos, así como de la cantidad de agua añadida al medio de reacción, en la producción de monoacilglicéridos. De los resultados obtenidos, se puede concluir que, para una relación molar inicial de reactantes glicerol:aceite de sardina de 3:1, un 12 % en peso de agua en base al glicerol y un 10 % en peso de lipasa, en base al peso de reactantes; se puede llegar a conseguir un rendimiento en monoacilglicéridos alrededor del 70 % en peso, con casi un 28 % en peso de AGPI y un bajo contenido en ácidos grasos libres (menor del 18 % en peso).

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