Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

Solaesa, Ángela García; Sanz, Marı́a Teresa; Falkeborg, Mia; Beltrán, Sagrario; Guo, Zheng

doi:10.1016/j.foodchem.2015.06.061

Cited by 104 publications

(60 citation statements)

References 40 publications

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“…Similar levels of enrichment, with best lipases, have been observed after 50 to 80% hydrolysis [68]. In the studies where higher efficiencies in concentrating ω-3 FAs were observed, these efficiencies were shown to be achieved by repeated hydrolysis [69], trans-esterification with ω-3 FAs [70], or combining lipase catalysis with other methods [71,72]. Taiwo et al have achieved up to 80% ω-3 FAs concentration in fish oil hydrolysate [73].…”

Section: Discussionmentioning

confidence: 68%

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Moharana

Rao

2020

PLoS ONE

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Enrichment of omega-3 fatty acids (ω-3 FAs) in natural oils is important to realize their health benefits. Lipases are promising catalysts to perform this enrichment, however, fatty acid specificity of lipases is poor. We attempted to improve the fatty acid selectivity of a lipase from Geobacillus thermoleovorans (GTL) by two approaches. In a semi-rational approach, amino acid positions critical for binding were identified by docking the substrate to the GTL and best substitutes at these positions were identified by site saturation mutagenesis followed by screening to obtain a variant of GTL (CM-GTL). In the second approach based on rational design, a variant of GTL was designed (DM-GTL) wherein the active site was narrowed by incorporating two heavier amino acids in the lining of acyl-binding pocket to hinder access to bulky ω-3 FAs. The affinities DM-GTL with designed substrates were evaluated in silico. Both, CM-GTL and DM-GTL have shown excellent ability to discriminate against the ω-3 FAs during hydrolysis of oils. Engineering the binding pocket of an enzyme of a complex substrate, such as a triglyceride, by incorporating the information on substrate structure and computationally derived binding modes, has resulted in designing two efficient lipase variants with improved substrate selectivity.

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Section: Discussionmentioning

confidence: 68%

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Moharana

Rao

2020

PLoS ONE

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“…Similar levels of enrichment, with best lipases have been observed after 50 to 80% hydrolysis [67]. In the studies where higher efficiencies in concentration of ω-3 FAs were observed, these efficiencies were shown to be achieved by repeated hydrolysis [68], trans-esterification with ω-3 FAs [69], or combining lipase catalysis with other methods [70,71]. Taiwo et al have achieved up to 80% ω-3 FAs concentration in fish oil hydrolysate [72].…”

Section: Discussionmentioning

confidence: 69%

Substrate structure and computation guided engineering of a Lipase for Omega-3 fatty acid selectivity

Moharana

Rao

2019

Preprint

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AbstractOptimum health benefits of omega-3 fatty acids (ω-3 FAs) require it to be concentrated in its natural sources. Fatty acid selectivity of lipase governs the efficacy of the production of lipase-mediated ω-3 FAs concentrates. We attempted to improve the fatty acid selectivity of a lipase from thermophilic bacteriumGeobacillus thermoleovorans(GTL) by two approaches. In a semi-rational approach, six amino acid positions of GTL interacting with the substrate, were identified by docking and were subjected to site-saturation mutagenesis. Three best substitutions were incorporated into GTL(CM-GTL). Hydrolysis of oil by lipase was monitored in a pH-Stat and the fatty acids released at various time points were analyzed by GC-MS.CM-GTL showed a significant improvement in discrimination against DHA during hydrolysis. In the second approach based on rational design, the active site was narrowed by incorporating heavier amino acids in the lining of acyl-binding pocket to hinder access to bulky ω-3 FAs. For this purpose, two amino acids surrounding the opening of the acyl pocket were replaced with the next heavier amino acids and the affinities were evaluatedin silico.The double mutant, thus deigned, was found to be excellent in discriminating the ω-3 FAs during hydrolysis of triglycerides. Engineering the binding pocket of a complex substrate, such as a triglyceride, with the supportive information on substrate structure and its binding modes with the enzyme provided by computational methods, has resulted in designing two efficient lipase variants with improved substrate selectivity.

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“…Some articles could thus be listed in both sections; however, repetition is avoided here and also in this case tabulation is based on the same fundamental key for the analysis of literature adopted previously in Table 1; enzyme (marine or commercial)-based bioprocesses and types of marine-originating feedstocks are the two columns of Table 2, including a third one for notes [62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96]. Commercial or marine-originating proteases and lipases are most used for production in this field and a wide range of edible biomass for the valorization study is listed in Table 2.…”

Section: Food Applicationsmentioning

confidence: 99%

Enzymatic Processes in Marine Biotechnology

Trincone

2017

Marine Drugs

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In previous review articles the attention of the biocatalytically oriented scientific community towards the marine environment as a source of biocatalysts focused on the habitat-related properties of marine enzymes. Updates have already appeared in the literature, including marine examples of oxidoreductases, hydrolases, transferases, isomerases, ligases, and lyases ready for food and pharmaceutical applications. Here a new approach for searching the literature and presenting a more refined analysis is adopted with respect to previous surveys, centering the attention on the enzymatic process rather than on a single novel activity. Fields of applications are easily individuated: (i) the biorefinery value-chain, where the provision of biomass is one of the most important aspects, with aquaculture as the prominent sector; (ii) the food industry, where the interest in the marine domain is similarly developed to deal with the enzymatic procedures adopted in food manipulation; (iii) the selective and easy extraction/modification of structurally complex marine molecules, where enzymatic treatments are a recognized tool to improve efficiency and selectivity; and (iv) marine biomarkers and derived applications (bioremediation) in pollution monitoring are also included in that these studies could be of high significance for the appreciation of marine bioprocesses.

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Production and concentration of monoacylglycerols rich in omega-3 polyunsaturated fatty acids by enzymatic glycerolysis and molecular distillation

Cited by 104 publications

References 40 publications

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Substrate structure and computation guided engineering of a lipase for omega-3 fatty acid selectivity

Substrate structure and computation guided engineering of a Lipase for Omega-3 fatty acid selectivity

Enzymatic Processes in Marine Biotechnology

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