Liquid gated ZnO nanorod FET sensor for ultrasensitive detection of Hepatitis B surface antigen with vertical electrode configuration

Chakraborty, Bidesh; Ghosh, S. K.; Das, Naren; RoyChaudhuri, C.

doi:10.1016/j.bios.2018.09.019

Cited by 20 publications

(12 citation statements)

References 44 publications

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“…A comparison of this method with other methods developed for the detection of HBsAg is given in Table 1. These comparisons illustrate that the Au@UiO-66(NH 2 ) composite-based electrochemical sensor has several advantages, including a LOD and LDR for HBsAg when evaluated against most of the electrodes modified with different nanomaterials [37][38][39][40][41][42][43][44][45][46][47][48]. The previously reported sensors based on other nanomaterials showed good performance, but the better performance of the Au@UiO-66(NH 2 ) composite may be attributed to the large surface area of UiO-66(NH 2 ), high electrocatalytic activity of Au, and the fast electron transfer mediation from the immobilized HBsAg.…”

Section: Sensitivity Of the Developed Electrochemical Immunosensing Assay Against Hbvmentioning

confidence: 94%

Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

et al. 2021

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An ultrasensitive electrochemical immunosensor has been prepared using an immunofunctionalized zirconium (Zr)-based metalorganic framework (MOF) with gold (Au) decoration Au@UiO-66(NH 2 ) composite-coated glassy carbon electrode (GCE) for the determination of infectious hepatitis B surface antigen (HBsAg). We fabricated GCE with specific composite via immunefunctionalization using anti-HBsAg with Au nanoparticles embedded in UiO-66(NH 2 ). The electrochemical sensing performance of the immunofunctionalized Au@UiO-66(NH 2 )/GCE with HBsAg was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, there was a linear dynamic relationship in the buffer system between the electrical signal and HBsAg levels over the range 1.13 fg mL −1 -100 ng mL −1 (R 2 = 0.999) with a detection limit of 1.13 fg mL −1 . The total analysis time was 15 min per sample. Further validations were performed with HBsAg-spiked human serum samples, and similar detection limits as in the buffer system were observed with reduced signal intensities at lower concentrations of HBsAg (1, 10, and 100 fg mL −1 ) and minimal interference. The HBsAg electrochemical immunosensing assay had good selectivity and excellent reproducibility, thereby indicating its significant potential in the super-fast diagnosis of hepatitis B.

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Section: Sensitivity Of the Developed Electrochemical Immunosensing Assay Against Hbvmentioning

confidence: 94%

Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

et al. 2021

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“…28 After that, ZnO nanorods were grown on the seeded area following a previously reported procedure. 15 Then, 50 mM zinc nitrate hexahydrate [Zn(NO 3 ) 2 •6H 2 O] was mixed in 50 mL of DI water and stirred for 10 min. After this, 50 mM hexamethylenetetramine (HMTA: C 6 H 12 N 4 ) was mixed with 50 mL of DI water, and this was also stirred for 10 min.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Competitive Impedance Spectroscopy in a Schottky-Contacted ZnO Nanorod Structure for Ultrasensitive and Specific Biosensing in a Physiological Analyte

et al. 2022

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To enable detection and discovery of biomarkers, development of label-free, ultrasensitive, and specific sensors is the need of the hour. For addressing this requirement, here, a Schottky-contacted ZnO nanorod biosensor has been demonstrated, which explores the interplay between Schottky junction capacitance and solution resistance, resulting in an interesting sensing principle of competitive impedance spectroscopy. When the transition of dominating impedance occurs from solution resistance to junction capacitance, a notch or a peak appears in the impedance response at a particular frequency (referred to as the corner frequency) depending on the charge of the target molecule. The appearance of the peak or notch acts like an electronic label for selectivity since it is visible only for target molecules even at ultralow concentrations in the physiological analyte, where the magnitude of impedance change overlaps with that for nonspecific molecules. This phenomenon has been successfully applied for the positively charged vascular endothelial growth factor (VEGF) and the negatively charged hepatitis B surface antigen (HBsAg), where the shifts in the higher corner frequencies for 1 aM concentration of the target molecules have been observed to be more than 3 times the changes in the impedance magnitude. Further, the area of the ZnO nanorods was segmented into two zones corresponding to the lower and higher concentration regimes, thereby expanding the dynamic range. To summarize, an ultralow detection limit of 1 aM with a dynamic range up to 1 pM was achieved for VEGF and HBsAg, which is 4 orders of magnitude and 20 times lower than their most sensitive label-free reports, respectively.

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“…Besides silicon, other materials have been explored in the development of FET immunosensors based on NWs, such as indium phosphide (InP) [55] and zinc oxide (ZnO) [129][130][131]. In the III-V semiconductor family, InP NWs exhibit exciting features for the development of FET-based immunosensors.…”

Section: Nanowires-silicon and Beyondmentioning

confidence: 99%

“…One of the distinctions of ZnO NRs is their use to build FET in a non-planar configuration. Chakraborty et al [130] showed the effect of the horizontal and vertical drain-source contact configuration in the sensitivity of a ZnO-NR-FET-based immunosensor for the ultrasensitive detection of Hep-B antigen biomarkers. First, the authors covalently immobilized the anti-Hep-B monoclonal antibodies on the ZnO NRs surface using the 3-mercaptopropyltrimethoxysilane (MTS).…”

Section: Nanowires-silicon and Beyondmentioning

confidence: 99%

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Addressing the Theoretical and Experimental Aspects of Low-Dimensional-Materials-Based FET Immunosensors: A Review

et al. 2021

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Electrochemical immunosensors (EI) have been widely investigated in the last several years. Among them, immunosensors based on low-dimensional materials (LDM) stand out, as they could provide a substantial gain in fabricating point-of-care devices, paving the way for fast, precise, and sensitive diagnosis of numerous severe illnesses. The high surface area available in LDMs makes it possible to immobilize a high density of bioreceptors, improving the sensitivity in biorecognition events between antibodies and antigens. If on the one hand, many works present promising results in using LDMs as a sensing material in EIs, on the other hand, very few of them discuss the fundamental interactions involved at the interfaces. Understanding the fundamental Chemistry and Physics of the interactions between the surface of LDMs and the bioreceptors, and how the operating conditions and biorecognition events affect those interactions, is vital when proposing new devices. Here, we present a review of recent works on EIs, focusing on devices that use LDMs (1D and 2D) as the sensing substrate. To do so, we highlight both experimental and theoretical aspects, bringing to light the fundamental aspects of the main interactions occurring at the interfaces and the operating mechanisms in which the detections are based.

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Liquid gated ZnO nanorod FET sensor for ultrasensitive detection of Hepatitis B surface antigen with vertical electrode configuration

Cited by 20 publications

References 44 publications

Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen

Competitive Impedance Spectroscopy in a Schottky-Contacted ZnO Nanorod Structure for Ultrasensitive and Specific Biosensing in a Physiological Analyte

Addressing the Theoretical and Experimental Aspects of Low-Dimensional-Materials-Based FET Immunosensors: A Review

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