Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains

Mascarin, Gabriel Moura; Jackson, Mark A.; Kobori, Nilce Naomi; Behle, Robert W.; Delalibera, Ítalo

doi:10.1016/j.jip.2014.12.001

Cited by 79 publications

(65 citation statements)

References 29 publications

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“…We note that most commercial preparations of I. fumosorosea rely on blastopore preparations which are less expensive to produce using liquid culture fermentation (Mascarin et al, 2015). Blastospores have different physiochemical properties than conidia, and are not known to produce appressoria (Dunlap et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity of Isaria fumosorosea to Bemisia tabaci, with some observations on the fungal infection process and host immune response

Tian

Diao

et al. 2015

Journal of Invertebrate Pathology

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Section: Discussionmentioning

confidence: 99%

Pathogenicity of Isaria fumosorosea to Bemisia tabaci, with some observations on the fungal infection process and host immune response

Tian

Diao

et al. 2015

Journal of Invertebrate Pathology

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“…Recent advances in LSF have shown that concentrations of 1 × 10 9 blastospores per ml of B. bassiana can be produced in 3 days under laboratory conditions (Mascarin et al . ). Although the production of conidia by this fungus is strain dependent, the same concentration of this infective cell was achieved in our system after a relatively short time (7 days).…”

Section: Discussionmentioning

confidence: 97%

A nonconventional two-stage fermentation system for the production of aerial conidia of entomopathogenic fungi utilizing surface tension

2018

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Aim: To describe a new approach in which production of conidia of an entomopathogenic fungus takes place on the surface of an unstirred shallow liquid culture kept in nonabsorbent wells distributed in plastic sheets resembling a honeycomb. Methods and Results: First, liquid incubation time and medium composition for production of Beauveria bassiana aerial conidia were optimized. Wells inoculated with Sabouraud dextrose yeast extract produced 2Á2 9 10 8 conidia per cm 2 of liquid surface following 5 days of incubation. Finally, tests were carried out in a prototype comprised of stacked plastic sheets in a cylindrical container. Conidia production on liquid culture surface varied from 1Á2 to 1Á6 9 10 9 conidia per ml of fermented broth. Germination rates and insect activity towards Tenebrio molitor larvae were not negatively affected when compared to conidia produced on solid medium. Conclusions: The two-stage fermentation process here described, based on a simple nonabsorbent inert support, has potential for the application in the production of aerial conidia of B. bassiana and other fungi. Significance and Impact of the Study: Aerial conidia are the most extensive propagule type used in commercial mycopesticides, traditionally produced by solid-state fermentation (SSF). The industrial applications and other important benefits of the two-stage fermentation process here described may overcome some hurdles inherent to SSF aiming for the production of aerial conidia. Additionally, production consistency is increased by the use of chemically defined medium, and the better control of the environmental conditions could allow for more reproducible industrial batches.

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“…Selected for the application (JENKINS et al, 1998). To improve growth and sporulation of entomopathogenic fungi such as B. bassiana, M. anisopliae and I. fumosorosea nutritional studies were carried out (GRABOWSKI-OCAMPOS et al, 2005;VILLALBA et al, 2009;MASCARIN et al, 2015), but in I. fumosorosea its ability to grow and produce conidia in multiple sources of carbon and nitrogen is a factor that allows its mass production to be relatively inexpensive (CLIQUET; JACKSON, 1999).…”

Section: Introductionmentioning

confidence: 99%

Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

et al. 2019

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In the last decades, the use of entomopathogenic fungi for the control of pest insects has increased globally, however a key step to achieve the successful application of fungal propagules as biocontrol agents depends of various factors, inoculum production being one of the essential stages for these microorganisms to be used in pest management programs. The objective of this study was to evaluate the production of conidia by solid fermentation and biphasic culture in different sporulation matrices for four native isolates and a strain of Isaria fumosorosea that have been shown potential for the control of various insects at the laboratory level. The experimental design was completely randomized, two propagation methods were used with six treatments and ten repetitions per treatment; the data were analyzed using an analysis of variance and the means were compared using the Tukey test (p≤ 0.05). In the production by solid fermentation the minimum value of 5.30 × 10 5 conidia g -1 was registered with the pericarp of peanut and the maximum of 2.35 × 10 7 conidia g -1 in the corn grain; in the biphasic culture the minimum of 7.60 × 10 5 conidia g -1 was observed in birdseed and the maximum of 2.07 × 10 7 conidia g -1 in rice. The differences were significant (p ≤ 0.05) in the production by method and by substrate, in solid fermentation 6.84 × 10 6 conidia g -1 and in biphasic culture 8.85 × 10 6 conidia g -1 . In the production by substrate, the rice showed 1.75 × 10 7 conidia g -1 and the lesser canary seed concentration (7.80 × 10 5 conidia g -1 ). The average production per isolate and / or strain was of the order of 10 6 and significant difference was registered (p ≤ 0.05) among the fungi, the isolate HIB-9 showed concentration in average higher in the production (7.90 × 10 6 conidia g -1 ) and the isolated HIB-19 was the least effective, with only 1.08 × 10 6 conidia g -1 . The results obtained show marked differences between the isolates with respect to their capacity to use the different substrates used as propagation media to obtain conidia of I. fumosorosea.

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Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains

Cited by 79 publications

References 29 publications

Pathogenicity of Isaria fumosorosea to Bemisia tabaci, with some observations on the fungal infection process and host immune response

Pathogenicity of Isaria fumosorosea to Bemisia tabaci, with some observations on the fungal infection process and host immune response

A nonconventional two-stage fermentation system for the production of aerial conidia of entomopathogenic fungi utilizing surface tension

Conidia production of Isaria fumosorosea (Hypocreales: Cordycipitaceae) in organic substrates through two propagation techniques

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